[PDF] Top 20 Inhibition of Herpes Simplex Virus Type 2 Replication by Thymidine

... The rate of formation of dCMP in cell extracts prepared from uninfected KB cells and KB cells infected with HSV-1 or HSV-2 was linear for at least 25 min and was proportional to extract [r] ... See full document

... WT virus on a retrograde course from the skin to the ganglia, where the WT virus replicated and produced viral ...the replication deficiency of the HSV-1 tk mutant(s) in trans and help the mutants to ... See full document

... Herpes simplex virus (HSV) mutants defective for thymidine kinase expression (TK 2 ) have been reported to establish latent infection of sensory ganglia of mice, in that HSV ... See full document

... effectors, 2 m g of either an anti-Fas monoclonal antibody that inhibits apoptosis mediated through the Fas ligand-Fas pathway (Immunotech, Westbrook, Maine) or an isotype-matched control monoclonal antibody per ... See full document

... GALLOWAY* Fred Hutchinson Cancer Research Center, Seattle, Washington 98104 We have determined the complete nucleotide sequence of the thymidine kinase gene of herpes simplex virus HSV t[r] ... See full document

... Us11-deficient virus or the IE-Us11 recombinant in the presence and absence of ...Us11-deficient virus ( ⌬ Us11) was unable to suppress OAS activity in extracts prepared from IFN-treated NHDFs in the ... See full document

... VP19C is a capsid protein of Herpes simplex virus (HSV) type 1, which is essential for the assembly of the capsid shell structure. If VP19C is absent, the capsid shells do not form. VP19C is ... See full document

... of replication-competent controlled ...other replication-essential vi- ral genes than ICP4 and ICP8 are subjected to the control of a dually responsive gene ...of virus replication. ... See full document

... After purification of the insert from pBglO, digestion with Sall lane 5 gave the internal 1,680-bp SalI fragment and, in addition, a small amount of deleted SalI fragment migrating sligh[r] ... See full document

... immunodeficiency virus and pMD-G expressing the envelope G protein of vesicular stoma- titis virus (VSV), provided by Kyeong Ho Lee (Yonsei University Wonju Campus, Wonju, Republic of ...At 2 days ... See full document

... Adeno-associated virus type 2 (AAV), a member of the parvovirus family, contains a single-stranded genome of 4,679 ...the replication (Rep) proteins and the other coding for the structural ... See full document

... Herpes simplex virus type 1 thymidine kinase exhibits a strikingly broad substrate ...human thymidine kinase, deoxycytidine as does human deoxycytidine kinase, the cytosolic ... See full document

... during replication to generate progeny whose genomes consist of genetic segments obtained from both parental ...DNA replication (67) and different cell factors (14, ...rabies virus, bovine ... See full document

... HSV-1 replication by regulating all stages of mRNA biogenesis, including transcription, RNA processing, and export for translation ...mutant virus at 2 h postinfection, suggested that p53 played a ... See full document

... Western blotting. Protein expression was examined by Western blot analysis. Detection of Pol protein was carried out by infecting HEp-2 cells with various viruses bearing UL30 mutations at a multiplicity of ... See full document

... viral replication cycle. The results of the study with HSV-2 are similar to the findings of the HSV-1 study (Figure ...HSV-2 replication and prevent completion of the viral lytic ... See full document

... genome replication in the presence of ade- novirus and that this enzyme is also able to drive AAV repli- cation in vitro (40, ...AAV replication in live cells (1, 78, ...another virus has already ... See full document

... The fidelity of DNA replication is regulated by several dif- ferent mechanisms (reviewed in reference 14 and references therein). In particular, the selection of correct nucleotides by DNA polymerase (Pol) serves ... See full document

... the cleaved product of PARP (BD Biosciences, San Jose, CA) followed by flow cytometry. Consistent with the above results, the highest percentage of apoptotic cells was seen in cells transfected with the HSV-2 ... See full document

... In addition, survival rates determined from plating efficiencies of the various cell mutants after incubations at 39.5°C for the time periods corresponding 42 Downloaded from http://jvi.[r] ... See full document