[PDF] Top 20 Interactions of Herpes Simplex Virus Type 1 with ND10 and Recruitment of PML to Replication Compartments

... HSV replication genes, repli- cation compartments form adjacent to presumably intact ND10 ...viral replication compartments formed at sites adjacent to ...infection, ND10 dis- ... See full document

... The PML staining observed in stage III foci and in the replication com- partments in HEp2 cells is not due to cross-reactivity of the PML antibody with viral proteins, because no PML staining ... See full document

... the herpes simplex virus (HSV) type 1 replication apparatus leading to replication compartments likely involves the initial assembly of five viral ... See full document

... with ND10 structures and mediates their disruption (Everett & Maul, 1994 ; Maul & Everett, 1994 ; Maul et ...of PML (Everett et ...called replication compartments near the former sites ... See full document

... wild-type virus in the presence of ...to ND10 (18, 35) and that replication compartments form at the periphery of ND10 (18, 35), we were interested in the relation- ship of the ... See full document

... of PML has no detrimental effect on HSV-1 infection (6, 23), and the replication of neither wild-type (wt) nor ICP0-negative HSV-1 viruses is improved in mouse PML ⫺/⫺ ... See full document

... degrade PML (21), treatment with HDAC inhibitors (27), inhibition of DNA replication (31), or the presence of an ICP27 mutant ...of PML in infected cells showed clearly that ND10s were efficiently ... See full document

... genome replication in the presence of ade- novirus and that this enzyme is also able to drive AAV repli- cation in vitro (40, ...AAV replication in live cells (1, 78, ...(Table 1) and colocal- ... See full document

... this recruitment in PML-positive cells does not require viral gene expression ...other ND10 proteins might be relo- cated to the sites of in1374 genomes even in the absence of PML during the ... See full document

... 30). ND10 are dynamic structures that disassemble during mitosis and apoptosis and are structurally affected by many forms of stress, such as heat shock, heavy metal treat- ment, and a variety of drugs that alter ... See full document

... into replication com- partments must contain at least one parental viral genome, it is uncertain whether all such foci contain viral ...to ND10 implies that at a high multiplicity of infection more than one ... See full document

... with ND10. Interestingly, Maul et al. have shown that HSV-1 DNA localizes adjacent to ND10 in the absence of viral transcription and translation ...to ND10. If UL29 localizes adjacent to ... See full document

... lytic virus infection prompted the hy- pothesis that ND10 might have a repressive effect on HSV-1 gene expression and thereby constitute an intrinsic antiviral ...of PML isoforms III, IV, and ... See full document

... both replication- related proteins and viral DNA in HSV-1 infection, although neither is essential for viral DNA replication per se in transient replication ...viral replication ... See full document

... and ND10. The question arose as to whether the viral transcription at ND10 could be due to binding of ND10-asso- ciated proteins to the incoming viral origin of replication, fol- lowed by ... See full document

... an ND10 structure and/or a specific location at which a replication com- partment could begin to develop, upon reflection this model presents a significant ...preexisting ND10 foci, that is, with ... See full document

... the herpes simplex virus type 1 (HSV-1) genome might be expected to induce a DNA damage response, the ATR kinase is not activated in infected ...HSV-1 replication ... See full document

... viral replication proteins and several cellular replication pro- teins which are now known to colocalize within replication compartments, ICP4 and several cleavage and packaging pro- teins are ... See full document

... Replication products were also examined by two-dimen- sional agarose gel electrophoresis (Fig. 3B). The products were first separated under neutral conditions. In the second dimen- sion, electrophoresis was ... See full document

... These data are consistent with the view that the genes ULS, UL8, UL9, UL42, and ULS2, like the genes encoding ICP8 and polymerase 12, 15, are all delayed-early genes, as expected for our[r] ... See full document