[PDF] Top 20 Isolation and preliminary characterization of temperature-sensitive mutants of poliovirus type 1.

... When the RNA species produced during infection by either wild-type virus or tsB9 at the permissive temperature were examined by agarose gel electrophoresis, no difference was seen.. At t[r] ... See full document

... Little or no infectious virus was produced in cells infected with group I or group III mutants and shifted to 33.5 C in the presence of 5-AC, indicating little or no virion RNA synthesis[r] ... See full document

... Mutants were assigned to complementation groups by observing lysis at 29 C in bacteria mixedly infected with two temperature-sensitive mutants.. The mixture was left for 30 min for adsor[r] ... See full document

... Temperature-sensitive mutants of influenza A virus: isolation of mutants and preliminary observations on genetic recombination and complementation.. Influenza viral RNA and ribonucleopro[r] ... See full document

... 27/35 of all the mutants tested thus far [unpubdata ] are RNA+ is similar to that found tants; group C contains one RNA+ mutant that lished ts mutants of vesicular stomatitis virus, a is[r] ... See full document

... I have isolated temperature-sensitive mutants of encephalomyocarditis EMC virus because I wished to find mutants where the cleavage of viral precursor proteins is defective in mutant-inf[r] ... See full document

... DISCUSSION The present investigation describes the isolation of temperature-sensitive mutants of human Ad2, their division into 13 complementation groups, and the construction of a provi[r] ... See full document

... STEWART Department of Biology, Rice University, Houston, Texas 77001 A procedure is described for the selective isolation of temperature-sensitive, replication-deficient mutants of Bacil[r] ... See full document

... The group I prototype mutant tsl7 was able to synthesize viral polypeptides at the nonpermissive temperature 39.5°C, but the level of synthesis was less than that given by wild-type viru[r] ... See full document

... Synthesis of the viral DNA strand was shown to be diminished for both wild-type virus and these mutants at the restrictive temperature of 39.5 C, but the mutants were more defective than[r] ... See full document

... of poliovirus type 1 (OM) is generally unable to cause paralysis in ...in poliovirus-sensitive transgenic mice inoculated with ... See full document

... Analysis of protein fractions in cells infected with the ts 5 mutant, which at 40 C induced synthesis of RNA and antigen Table 1, revealed formation of protein structures with sedimentat[r] ... See full document

... It can be seen that ts 3 fails to synthesize any of the capsid antigens; ts 11, ts 4, and ts 7 representing three distinct complementation groups produced normal precipitin lines corresp[r] ... See full document

... The means calculated elevated temperature, and only 16% of the polyfrom several experiments for the cellular DNA merizing activity remained after 20 min at 45°C, synthesized at 39.5°C re[r] ... See full document

... We found that inclusion of Act D at 1 ,u.g/ml three sources in the inoculum during challenge of HeLa cell monolayer cultures inhibited replication of the parent and prototype ts mutant v[r] ... See full document

... Preliminary biochemical and immunological characterization of the mutants indicated that: i the single mutant in complementation group I did not replicate its deoxyribonucleic acid DNA o[r] ... See full document

... Intertypic complementation tests with temperature-sensitive mutants of adenovirus type 5 showed that the mutant in complementation group A failed to complement H5ts125 a DNA-binding prot[r] ... See full document

... SDS-PAGE analysis of extracts of Vero or n-33 cells infected at a multiplicity of infection of 20 PFU per cell with hr259, hr79, or HSV-2 186 and labeled with [35S]methionine from 3 to 1[r] ... See full document

... 704 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Biochemical transformation assays of herpes simplex virus type 1 temperaturesensitive ts mutants distinguished three[r] ... See full document

... Mutants representing all seven complementation groups failed to grow in either of these two transformed cell lines at the nonpermissive temperature, as measured by plaque formation on th[r] ... See full document