[PDF] Top 20 Leucine-Specific, Functional Interactions between Human Immunodeficiency Virus Type 1 Nef and Adaptor Protein Complexes
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Leucine-Specific, Functional Interactions between Human Immunodeficiency Virus Type 1 Nef and Adaptor Protein Complexes
... HIV-1 Nef with distinct motifs from cellular proteins and correlated protein interactions with biologic ...of Nef provided a structurally favorable context for the presentation of a ... See full document
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Functional Interactions of Human Immunodeficiency Virus Type 1 Integrase with Human and Yeast HSP60
... The protein extracts were obtained from yeast strain JSC310 (MAT␣ ura3-52 leu2 trp1 prb1-1122 pep4-3 prc1–407) or JSC310 (IN) transformed with pHIV1-SF2IN ... See full document
10
Association of Nef with the Human Immunodeficiency Virus Type 1 Core
... the human immunodeficiency virus type 1 (HIV-1) Nef protein enhances viral infectivity by an unknown ...mechanism. Nef-defective virions are blocked at a ... See full document
7
Human Immunodeficiency Virus Type 1 Nef Incorporation into Virions Does Not Increase Infectivity
... a functional form of Nef is important during the biogenesis of viral particles ...a Nef-Vpr fusion protein containing a protease specific cleavage site between Nef and ... See full document
12
Biochemical Analyses of the Interactions between Human Immunodeficiency Virus Type 1 Vpr and p6Gag
... Vpr protein in a concentration- and Vpr-dependent ...Vpr-p6 complexes dissociated (and associated) very rapidly, with the half-lives of both complexes being less than 1 ...simple ... See full document
6
Human immunodeficiency virus type 1 Nef does not alter T-cell sensitivity to antigen-specific stimulation.
... that Nef was both intact and expressed at physiologically normal levels in the cell lines A nef1, M nef1, and M nef12, the level of Nef protein was measured by immunoblot ...which Nef would be ... See full document
12
The Barrier-to-Autointegration Factor Is a Component of Functional Human Immunodeficiency Virus Type 1 Preintegration Complexes
... by specific interactions between an- ...(lane 1) and PIC ...lanes 1 and 2, the fraction II samples shown in lanes 3 to 12 were purified by spin column ... See full document
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Downregulation of CD4 by Human Immunodeficiency Virus Type 1 Nef Is Dependent on Clathrin and Involves Direct Interaction of Nef with the AP2 Clathrin Adaptor
... of Nef with AP2 demonstrated in a yeast thee-hybrid ...of Nef effects on ...of Nef with AP2. We had previously reported interactions of Nef with the ␥ - 1 and ␦ - 3 ... See full document
14
Genetic and Functional Diversity of Human Immunodeficiency Virus Type 1 Subtype B Nef Primary Isolates
... 233 Nef, D88-11 Nef, and D90-7 Nef all failed to bind ...the protein (not shown). In addition, the SF2K156E/E159K nef was constructed in an at- tempt to discover the defect in 233 ... See full document
9
Human Immunodeficiency Virus Type 1 Nef-Mediated Downregulation of CD4 Correlates with Nef Enhancement of Viral Pathogenesis
... These interactions seem to require distinct residues within Nef, situated at both the amino and carboxy termini of this viral regulatory ...of Nef have been proposed to interact with the cytoplasmic ... See full document
10
Functional Analysis of the Human Immunodeficiency Virus Type 1 Rev Protein Oligomerization Interface
... (residues 1 to 61–66), which contains the RNA binding-nuclear localization domain (98) as well as the putative multimerization domains (61, 64), is in an a-helical ...contacts between two a helices that are ... See full document
10
Interactions between Human Immunodeficiency Virus Type 1 and Vaccinia Virus in Human Lymphoid Tissue Ex Vivo
... Flow cytometry. Flow cytometry was performed on cells mechanically isolated from control and infected tissue blocks on day 12 after infection. Lymphocytes, identified according to their light-scattering properties, were ... See full document
7
Independent Segregation of Human Immunodeficiency Virus Type 1 Gag Protein Complexes and Lipid Rafts
... 2C. The Gag-GFP fusion construct expressing MA, CA, and SP1 fused to GFP did not associate with DRMs (Gag377/GFP [Fig. 2A]). In contrast, a similar construct that included just the first seven residues of NC was ... See full document
11
A role for natural simian immunodeficiency virus and human immunodeficiency virus type 1 nef alleles in lymphocyte activation.
... the nef locus, the four ATGs in the region of SIV- mac239 where the envelope and nef genes overlap were eliminated without altering the predicted Env amino acid ...of nef of HIV-1 NL4-3 and ... See full document
6
Requirement of human immunodeficiency virus type 1 nef for in vivo replication and pathogenicity.
... No depletion of CD4+ human thymocytes was observed in implants infected with either HIV-lJRCSF or its nef mutant at 3 or 6 weeks postinfection compared with controls consisting of mock-i[r] ... See full document
8
Nef stimulates human immunodeficiency virus type 1 proviral DNA synthesis.
... of virus particle formation. The reduced infectivity of D Nef HIV-1 can be complemented in trans by expressing nef in virus producer cells in a dose-depen- dent ...by Nef in the ... See full document
9
Nef Enhances Human Immunodeficiency Virus Type 1 Infectivity in the Absence of Matrix
... of virus were used to infect GHOST-CXCR4 cells, and virus production by the infected cells was then examined after metabolic labeling as described ...of Nef on virus production after a single ... See full document
6
Structure and expression of tat-, rev-, and nef-specific transcripts of human immunodeficiency virus type 1 in infected lymphocytes and macrophages.
... Southern blots of amplified cDNA from H9 cells chronically infected with the HTLV-IIIB strain of HIV-1 were hybridized with the different probes.. The most prominent band detected with t[r] ... See full document
8
Nef association with human immunodeficiency virus type 1 virions and cleavage by the viral protease.
... the nef gene due to the insertion of 4 ...LAI Nef protein, followed by 2 unrelated ...truncated Nef protein was stably expressed, Nef-related proteins were immu- noprecipitated ... See full document
6
DC-SIGN Interactions with Human Immunodeficiency Virus Type 1 and 2 and Simian Immunodeficiency Virus
... transmit human immunodeficiency virus (HIV) to cocultured T cells and so may play an important role in HIV ...HIV type 1 (HIV-1) strains and a laboratory-adapted X4 ...for ... See full document
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