[PDF] Top 20 Method To Detect Only Live Bacteria during PCR Amplification
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Method To Detect Only Live Bacteria during PCR Amplification
... rapid PCR methods to substitute for the culture method is a pressing matter in clinical and food hygiene ...the bacteria present in clinical samples and foods may be ...the bacteria of pas- ... See full document
9
Sensitive Detection of Borrelia burgdorferi Sensu Lato DNA and Differentiation ofBorrelia Species by LightCycler PCR
... LightCyler PCR and melting-curve analysis of the amplicons of two genes with intraspecies variability, the p66 gene and the recA gene, were ...LightCycler PCR amplification of p66 is more sensitive ... See full document
5
Simultaneous Analysis of Multiple Staphylococcal Enterotoxin Genes by an Oligonucleotide Microarray Assay
... and PCR, but these protocols were designed to detect only one or a few toxin genes (21, ...Multiplex PCR for detection of several ent genes has been reported (6, 26, 29, 30, 36), but ... See full document
10
Development of a Multiplex PCR Technique for Detection and Epidemiological Typing of Salmonella in Human Clinical Samples
... tiplex PCR methods was then performed on 120 consecutive human stool samples obtained at the Basurto Hospital, Bilbao, ...multiplex PCR, 100 l of the incubated broth was diluted in 10 ml of fresh broth and ... See full document
5
Immunomagnetic PCR and DNA probe for detection and identification of Porphyromonas gingivalis
... and PCR. A previous study by arbitrarily primed PCR identified a 1,146-bp amplicon (RP1) common to all strains of ...termination method with universal ...PCR amplification. Optimal ... See full document
5
Detection of Neospora from tissues of experimentally infected rhesus macaques by PCR and specific DNA probe hybridization
... using PCR technology we were able to detect Neospora DNA in many other tissue samples (Table ...the PCR-probe detection method, sug- gesting that only very small quantities of Neospora ... See full document
6
The Establishment of the PCR Detection Method of Babesia canis with Internal Amplification Control
... PCR was carried out using the pUC series universal Primer RV-M and Primer M13-47 to amplify the target band of 305 bp (lane 3 in Figure 1) using the plasmid pMD-18-TB.com-gallus as template. (Fig. 1, lane 1) can ... See full document
6
Evaluation of FLASH - PCR forrapid detection of Mycobacterium tuberculosis from clinical specimens.
... of PCR techniques (6) and many primer sets have been developed for specific detection of MTB genes (11, ...Fuorescent amplification- based specific hybridization PCR (FLASH-PCR) with the power ... See full document
8
Rapid DNA Extraction Protocol from Stool, Suitable for Molecular Genetic Diagnosis of Colon Cancer
... a PCR product suggesting the presence of impurities that inhibit PCR ...when PCR is used as a diagnostic method, not only because of the presence of numerous types of bacteria ... See full document
6
Detection of Human Immunodeficiency Virus Type 1 Antiretroviral Resistance Mutations by High Density DNA Probe Arrays
... particles, amplification by nested reverse transcriptase PCR, and detection with high-density probe arrays designed to detect 204 antiretroviral resistance mutations simultaneously in Gag cleavage ... See full document
6
Characterization of footrot bacteria Dichelobacter nodosus using PCR amplification and DNA sequence analysis
... Abstract: Dichelobacter nodosus is an essential causative agent of footrot in ruminants, particularly in sheep, goats and cattle. In this study, more than 100 footrot samples were collected from 4 different farming ... See full document
7
Original Article Development of a new real-time PCR system for simultaneous detection of bacteria and fungi in pathological samples
... To detect DNA sequences in FFPE samples, PCR amplicons should be less than 300 bp, because DNA is usually fragmented by formalin fixation [8, ...real-time PCR for detection of bacterial/fungal ... See full document
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Detection of Acanthamoeba and Toxoplasma in River Water Samples by Molecular Methods in Iran.
... isothermal amplification (LAMP) had been utilized for a broad spectrum of applications in the biomedical field including the detection of vi- ruses, bacteria, fungi and parasites (15, ...LAMP method ... See full document
8
Rapid Identification of Yersinia enterocolitica in Blood by the 5′ Nuclease PCR Assay
... Other bacteria impli- cated in RBC contamination are Pseudomonas putida, Campy- lobacter jejuni, Enterobacter jejuni, Escherichia coli, and Fla- vobacterium ...these bacteria (32). The bacteria go ... See full document
6
Detection and Genotyping of Human Rotavirus VP4 and VP7 Genes by Reverse Transcriptase PCR and Reverse Hybridization
... transcriptase PCR (RT-PCR) method for the amplification of rotavirus RNA and a reverse hybridization assay on a strip to detect amplimers and identify the specific G and P genotypes ... See full document
9
A METHODOLOGICAL COMPARISON AMONG DNA SOURCE TYPES FOR MOOSE GENOTYPING
... were stored in -20 °C freezers, but this has not proven entirely effective at eliminat- ing DNA degradation (Dawson et al. 1998), especially for extended periods. Storage beyond 6 months reduces both DNA yield and ... See full document
17
Nucleic Acid Sequence Based Amplification with Oligochromatography for Detection of Trypanosoma brucei in Clinical Samples
... to detect trypanosomes in 16 blood samples, of which 15 were from the Democratic Republic of the Congo ...and only 1 sample was from a ...not detect the ... See full document
6
Using PCR Amplification and Genetic Sequence Analysis of 18S rRNA Genes to Survey the Microbial Diversity and Distribution of Eukaryotic Microbes Inhabiting Two Thermo-acidic Streams in Yellowstone National Park, Wyoming
... temperature gradient study results, is unexpected. We reasoned that washout of C. merolae cells from upstream would result in the detection of at least a few sequences downstream. However, our results suggest that the ... See full document
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Identification of a granulocytotropic Ehrlichia species as the etiologic agent of human disease
... In order to detect and identify the etiologic agent of human granulocytic ehrlichiosis in patients 2, 3, 4, 5, and 6, we used the approach of nested PCR with initial amplification using [r] ... See full document
7
Definitive Differentiation between Single and Mixed Mycobacterial Infections in Red Deer (Cervus elaphus) by a Combination of Duplex Amplification of p34 and f57 Sequences and Hpy188I Enzymatic Restriction of Duplex Amplicons
... PCR controls. A strict procedure was followed to avoid cross-contamination between samples or carryover of PCR products. For any series of reactions, contamination at the DNA level was ruled out by ... See full document
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