[PDF] Top 20 Molecular Typing of Selected Enterococcus faecalis Isolates: Pilot Study Using Multilocus Sequence Typing and Pulsed Field Gel Electrophoresis
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Molecular Typing of Selected Enterococcus faecalis Isolates: Pilot Study Using Multilocus Sequence Typing and Pulsed Field Gel Electrophoresis
... for molecular epi- demiological analysis of ...characterizes isolates of bacteria using ⬃400 to 500 bp of intragenic sequences of six to seven housekeeping genes or loci, and thus, MLST is similar in ... See full document
9
Molecular Epidemiology of Hospital Acquired Vancomycin Resistant Enterococci
... Vancomycin-resistant Enterococcus faecium strains are a significant cause of nosocomial infections in pre- disposed ...with multilocus sequence typing (MLST) and pulsed-field ... See full document
5
Typing of Campylobacter jejuni isolates from Dogs using Multilocus Sequence Typing and Pulsed Field Gel Electrophoresis
... Multilocus sequence typing (MLST) is a tool used to investigate relationships between Campylobacter ...the molecular epidemiology of the disease (11, ... See full document
20
Comparison of Multilocus Sequence Typing, Pulsed Field Gel Electrophoresis, and Antimicrobial Susceptibility Typing for Characterization of Salmonella enterica Serotype Newport Isolates
... enterica using the same seven-gene MLST scheme used in this study and found that overall, discrimination was not improved within a serotype compared with PFGE and amplified fragment length polymorphism ... See full document
9
Multilocus Sequence Typing Lacks the Discriminatory Ability of Pulsed Field Gel Electrophoresis for Typing Salmonella enterica Serovar Typhimurium
... numerous typing methodologies have been developed. Among the several molecular typing methods, pulsed-field gel electrophoresis (PFGE) is currently considered the “gold ... See full document
5
Multilocus Sequence Typing versus Pulsed Field Gel Electrophoresis for Characterization of Extended Spectrum Beta Lactamase Producing Escherichia coli Isolates
... pathogens. Molecular typing of ESBL-producing ...Although pulsed-field gel electrophoresis (PFGE) is the current “gold standard” for bacterial molecular typing, ... See full document
6
Repetitive Sequence Based PCR versus Pulsed Field Gel Electrophoresis for Typing of Enterococcus faecalis at the Subspecies Level
... Repetitive sequence-based PCR was compared to pulsed-field gel electrophoresis (PFGE) for the ability to discriminate Enterococcus faecalis isolates at the ... See full document
5
Multilocus Sequence Typing Compared to Pulsed Field Gel Electrophoresis for Molecular Typing of Pseudomonas aeruginosa
... of typing that is discriminatory is essential for measuring the effectiveness of infection control ...of molecular typing. The present study compared the discriminatory abilities of ... See full document
6
Comparative Analysis of Multilocus Sequence Typing and Pulsed Field Gel Electrophoresis for Characterizing Listeria monocytogenes Strains Isolated from Environmental and Clinical Sources
... the isolates, because the data may be either noninformative, if the gene selected for analysis is highly con- served, or misleading, if the gene selected for analysis is highly ...ious ... See full document
10
Analysis of Enterococcus faecalis isolates from intercontinental sources by multilocus enzyme electrophoresis and pulsed field gel electrophoresis
... by typing techniques that demonstrate good discriminatory ...reliability. Pulsed-field gel electrophoresis (PFGE) has shown success in typing enterococci and has facilitated the ... See full document
5
Comparing Pulsed Field Gel Electrophoresis with Multilocus Sequence Typing, spa Typing, Staphylococcal Cassette Chromosome mec (SCCmec) Typing, and PCR for Panton Valentine Leukocidin, arcA, and opp3 in Methicillin Resistant Staphylococcus aureus Isolates at a U S Medical Center
... MRSA isolates were collected prospectively from the Clinical Microbiol- ogy Laboratory in 2008 from patients in multiple clinical settings (emer- gency department and inpatient and outpatient ...MRSA ... See full document
6
Genomic analysis of multidrug-resistant clinical Enterococcus faecalis isolates for antimicrobial resistance genes and virulence factors from the western region of Saudi Arabia
... and sequence reads were mapped to clinical isolate E. faecalis reference genome ...tree using CSI Phylogeny 1.4 [20]. The phylogenetic tree was visualized using Interactive Tree of Life (iTOL) ... See full document
11
Investigation of Kingella kingae Invasive Infection Outbreaks in Day Care Facilities: Assessment of a Rapid Genotyping Tool Targeting the DNA Uptake Sequence
... centers. Pulsed-field gel electrophoresis (PFGE) and multilocus se- quence typing (MLST) revealed that although the invasive strains had widespread dissemination in the day care ... See full document
9
Molecular Diagnostics in Clinical Microbiology
... (REP) sequence (61,62), the enter-- obacterial repetitive intergenic consensus (ERIC) sequence (63,64) or the BOX element ...studies using primers that target such repetitive sequences have ... See full document
16
Comparison of ribotyping and pulsed field gel electrophoresis for molecular typing of Acinetobacter isolates
... Acinetobacter isolates were studied; 46 were ...12). Using EcoRI, we found 31 different ribotypes among these ...the isolates were typeable, and ribotyping yielded between 10 and 15 bands per strain ... See full document
6
Comparison of Staphylococcus aureus Isolates from Bovine and Human Skin, Milking Equipment, and Bovine Milk by Phage Typing, Pulsed Field Gel Electrophoresis, and Binary Typing
... phage typing (18, 19), ribotyping (18, 23), multilocus enzyme electrophoresis (7, 15), random amplified polymorphic DNA typing (7), PFGE (3, 30, 43), coagulase gene typing (20), and ... See full document
9
A Core Genome Multilocus Sequence Typing Scheme for Enterococcus faecalis
... E. faecalis isolates, including those of various sampling backgrounds, such as hospital-associated strains and strains obtained from general screenings in outpatient settings (coloniza- tion), but also for ... See full document
12
Assessment of Candida glabrata Strain Relatedness by Pulsed Field Gel Electrophoresis and Multilocus Sequence Typing
... this study, 80 Candida glabrata isolates from intensive care unit and human immunodeficiency virus (HIV)-infected patients were typed by multilocus sequence typing (MLST), ... See full document
8
Comparison of Multiple Locus Variable Number Tandem Repeat Analysis with Pulsed Field Gel Electrophoresis, spa Typing, and Multilocus Sequence Typing for Clonal Characterization of Staphylococcus aureus Isolates
... two isolates of which were split into MLVA clusters M8 ⬘ and M9 ⬘ ...diverse isolates of this phylogenetic lineage included in the analysis, clusters M8 ⬘ and M9 ⬘ would form a single one also under the ... See full document
6
Development of a Canadian Standardized Protocol for Subtyping Methicillin Resistant Staphylococcus aureus Using Pulsed Field Gel Electrophoresis
... MRSA isolates collected by the CNISP (data not shown). No isolates to date have been identified as nontypeable using this proce- ...MRSA isolates displaying reduced susceptibility to ... See full document
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