[PDF] Top 20 Multiple Roles of Epstein-Barr Virus SM Protein in Lytic Replication

... DNA replication by transfected polymerase and primase allowed us to examine whether late genes were acti- vated solely by DNA ...late lytic genes is stimulated by DNA replication alone in the absence ... See full document

... by SM and were poorly expressed in its ...of lytic replication as shown in Fig. 1D. Although lytic gene expression was more robust in WT EBV, there was neverthe- less almost complete ... See full document

... to SM, or is it advantageous to viral replication? Although several of the ISGs, particularly those belonging to the ISG56K family, are among the genes most highly induced by IFN, little is known about ... See full document

... play multiple roles in constitutive and regulated splicing and con- tain one or two RNA-binding domains (RBDs) and a carboxy- terminal RS domain with various numbers of RS repeats ...of SM and SRp20, ... See full document

... between SM and DHX9. Since SM is critical for EBV lytic gene expression (11, 41) and DHX9 is involved in the replication of many viruses, we hypothesized that a change in DHX9 expression might ... See full document

... key roles in regulating the latent-lytic switch of Epstein-Barr Virus (EBV) by repressing BZLF1 gene ...into lytic replication by downregulating expression of ZEB1 and ... See full document

... Cells and antibodies. EBV-positive Burkitt’s lymphoma-derived P3HR-1 cells and the BJAB EBV-negative lymphoma cell line previ- ously described (30, 31) were a gift from Erle Robertson (University of Pennsylvania) and ... See full document

... and protein sequences is only significant in the N-terminal parts of the ...different roles in EBV biology; the significance of the binding to RBP-Jk would be to buffer the levels of the active ... See full document

... HHV8 lytic cycle protein that could activate other HHV8 lytic genes as well as host cell genes, we sought to identify and characterize the function of the HHV8 member of this family of ...EBV ... See full document

... membrane protein 1 (LMP1) of Epstein-Barr virus (EBV) is a transforming protein that affects multiple cell signaling pathways and contributes to EBV-associated ...during ... See full document

... ISRE probe. The purification process was done according to the method of our previous report, and partially purified K- RTA has the expected properties (91). The same amounts of partially purified proteins were used for ... See full document

... the lytic origin of DNA replication of herpes simplex virus type 1, in which DNA- protein interactions which seem to share partially the same DNA sequence requirements were mapped ... See full document

... C189 and other cysteine residues are sensitive to redox regulation of DNA binding. The DNA binding activity of Zta, like c-Jun, requires reducing agents, like DTT and ␤ -mercap- toethanol, to protect against cysteine ... See full document

... in lytic reactivation in EBV-infected cells is repair of DNA double-strand breaks gener- ated during lytic replication of viral ...During lytic reactiva- tion, the EBV DNA is replicated in a ... See full document

... late lytic cycle without the need for ori lyt in cis in the plasmids containing the late promoter being tested (26, ...the lytic cycle with different activity, but that would make it even more difficult to ... See full document

... that SM can partially compensate for ICP27 function, although the inability of vSM/TK-⌬27 to reach titers similar to those of v27/TK-⌬27 suggests that the two proteins are not completely ...of SM supports ... See full document

... viral lytic cycle, we expressed wild-type (wt) ZEBRA and the Z(S167A/S173A) mutant in BZKO cells that carry an EBV genome in which the BZLF1 gene, encoding ZEBRA, has been inactivated by insertional mutagenesis ... See full document

... EBV lytic-DNA ...Cellular protein and DNA extracts were prepared 96 h after ...cell protein extracts were probed for Rta, EA-D, and ...the replication of lytic viral DNA with a ... See full document

... induce lytic replication of EBV in the Burkitt’s lymphoma-derived cell line P3HR1/13 are ...entering lytic replication of EBV was measured with immunohistochemistry ...membrane protein ... See full document

... possible roles for ...revealed protein domains of LMP2B which are required for intra- and extracellular localization and self-aggregation (37), which raised the question of whether the function of LMP2B in ... See full document