[PDF] Top 20 Multivariate analysis of human immunodeficiency virus type 1 neutralization data.

... against human immuno- deficiency virus type 1 (HIV-1) infection remain ...tion data obtained with peripheral blood mononuclear cells (PBMCs) (15, 25, 30) and primary HIV-1 ... See full document

... effective neutralization as a greater-than-fivefold decrease in viral titer in a plasma ...of neutralization ranged from a 25- to a 625-fold re- duction in the original infectious ... See full document

... body neutralization, it was proposed that Env spike density could be an important modifier of sensitivity to antibody neu- tralization if this occurred through an occupancy/threshold mechanism, perhaps explaining ... See full document

... wild- type glycoproteins in the presence of ...wild- type envelope glycoproteins, this new conformation allows in- creased exposure of the 17b epitope; however, in a number of gp120 mutants in which the V2 ... See full document

... 3459 Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest Neutralization of a chimeric human immunodeficiency virus HIV type 1, containing the V3 loop of the MN isolate subst[r] ... See full document

... Amino acid sequence of N-AgI and viability of PV-1/HIV-1 PND chimeras Virus PV-1 Sabin vJFC 82 N-Agl amino acid sequence' 94 VDNSAS VDNSAS 105 TKNKDKLF TKNKDELF Length of the B-C loopb V[r] ... See full document

... of virus bound in the presence of serum versus the amount of virus bound without serum (black bar; the amount [picograms] of p24 associated with cells is indicated at the ...top). Neutralization was ... See full document

... for neutralization of HIV-1 JR-CSF, ...study, neutralization synergy of the same order of magnitude was reported by Mascola and colleagues for neutralization of a subset of clade B ... See full document

... (Fig. 1 and ...our data indicate that sufficient similarity exists be- tween the structures of the SIV and HIV-1 TM proteins such that HIV-derived epitopes are readily recognized by cognate ... See full document

... the virus-neutralizing capacity of antibodies to HIV-1 in hu- man ...that human MAb 2G12 broadly and potently neutralized the majority of clade B primary and T-cell line-adapted strains against which ... See full document

... MAb binding to intact and infectious PI and TCLA virions. The studies described above utilized molecularly cloned Envs expressed on the surface of transfected cells. These Envs are fully functional in mediating cell-cell ... See full document

... cytometric analysis of HIV-1 surface Env and intracellular p24 expres- ...wild-type virus or with MX2 virus as described ...in 1% paraformaldehyde for 1 ...of 1 ␮ g ... See full document

... against human immunode- ficiency virus type 1 (HIV-1) have attracted attention not only for the lessons they provide for designing vaccine antigens to induce a more robust immunological ... See full document

... to data described by Rossio et al. (39), where heating HIV-1 at 56°C for 2 h resulted in loss of binding to gp120 MAb IgG1b12, as measured by p24 assay of virions after precipitation with ...These ... See full document

... form virus-like particles (VLPs) and bud from cells in the absence of other viral components (5, ...after virus release, Gag is cleaved by the viral protease (PR) into the matrix (MA), capsid (CA), and ... See full document

... example, human immunodeficiency virus type 1 (HIV-1) encodes six accessory/regulatory genes in addition to the struc- tural and enzymatic gag, pol, and env genes that are present ... See full document

... of virus isolates is custom tailored, as it were, for resistance to autologous immune ...the virus and a remarkable and effective, albeit delayed, accommodation on the part of the immune ...culture ... See full document

... Unlike mutant D116A virions, however, viral particles produced by protease-defective HIV-1 28, murine leukemia virus 11, and avian leukosis virus 54 contain reverse transcriptase activit[r] ... See full document

... potent human MAbs (IgG1b12, 2G12, and 2F5) was as- ...FIG. 1. Envelope glycoproteins from distinct HIV-1 strains exhibit very different sensitivities to neutralization by anti-HIV-1-Env ... See full document

... clade-specific neutralization patterns (6, 10, 43, 58, 59, 71, 75, 90, 93, 102, ...positive neutralization data have meant that it has been diffi- cult to conduct a definitive ...on ... See full document