[PDF] Top 20 Mutagenesis Analysis of the rGTP-Specific Binding Site of Hepatitis C Virus RNA-Dependent RNA Polymerase
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Mutagenesis Analysis of the rGTP-Specific Binding Site of Hepatitis C Virus RNA-Dependent RNA Polymerase
... the rGTP-specific binding site on cell colony formation induced by HCV RNA ...catalyze RNA polymerization on both HCV-specific RNA and nonviral RNA templates ... See full document
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Structural Analysis of the Hepatitis C Virus RNA Polymerase in Complex with Ribonucleotides
... crystallographic analysis of complexes of the hepatitis C virus (HCV) RNA polymerase with ribonucleoside triphosphates (rNTPs) and divalent metal ...a specific rGTP ... See full document
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De Novo Initiation Pocket Mutations Have Multiple Effects on Hepatitis C Virus RNA-Dependent RNA Polymerase Activities
... less specific in the use of the NTPi, because the mutants still preferred ⫹ 1C for initiation and all of the analogs used possessed a guanine (Tables 1 and ...Mutational analysis of the NTPi residues also ... See full document
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The Hepatitis C Virus RNA-Dependent RNA Polymerase Membrane Insertion Sequence Is a Transmembrane Segment
... the C terminus of NS5B is translo- cated to the ER lumen also in the context of a functional HCV replication complex, the NS5B-gt construct was introduced into a subgenomic ...XbaI site was gener- ated ... See full document
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Regulation of De Novo-Initiated RNA Synthesis in Hepatitis C Virus RNA-Dependent RNA Polymerase by Intermolecular Interactions
... The C terminus of NS5B does not affect intermolecular ...a C-terminal histidine tag to aid in ...cleavage site immediately N-terminal to the histidine tag and subjected it to TEV protease ...for ... See full document
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Comparative Analysis of Hepatitis B Virus Polymerase Sequences Required for Viral RNA Binding, RNA Packaging, and Protein Priming
... Hε binding in vivo are fully consistent with our previous results obtained using an in vitro RNA binding assay with bacterially ex- pressed and chaperone-reconstituted HP ...the C-terminal ... See full document
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A Hepatitis C Virus NS5A Phosphorylation Site That Regulates RNA Replication
... pSGR-JFH1 RNA and a nonreplicative pSGR-JFH1 RNA containing a lethal lesion in the RNA-dependent RNA polymerase (Pol ⫺ ) ...course analysis of total HCV RNA accumu- ... See full document
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Specific Interaction between the Hepatitis C Virus NS5B RNA Polymerase and the 3′ End of the Viral RNA
... genomic RNA with or without the conserved 98-nucleotide tail. The NS5B-RNA complexes were specifically competed away by the unlabeled homologous RNA but not by the viral 5 ⴕ noncoding region and very ... See full document
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RNA-Binding Protein hnRNP D Modulates Internal Ribosome Entry Site-Dependent Translation of Hepatitis C Virus RNA
... HCV RNA) under the con- trol of the EMCV IRES ...blot analysis of Huh-luc/neo-ET cells transfected with control (con) or hnRNP D siRNA (si hnRNP ...blot analysis of Huh 5-15 cells transfected with ... See full document
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A Recombinant Hepatitis C Virus RNA-Dependent RNA Polymerase Capable of Copying the Full-Length Viral RNA
... Hepatitis C virus (HCV) is a positive-sense single-stranded RNA virus causing acute and chronic hepatitis in humans, frequently leading to liver cirrhosis and hepatocellular ... See full document
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De Novo Initiation of RNA Synthesis by the RNA-Dependent RNA Polymerase (NS5B) of Hepatitis C Virus
... vitro RNA transcription assay with either non-virus-specific RNA or genomic HCV RNA as templates (7, ...mixed virus pool with divergent NS5B se- quences that may result in ... See full document
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Selection of 3′-Template Bases and Initiating Nucleotides by Hepatitis C Virus NS5B RNA-Dependent RNA Polymerase
... Hepatitis C virus (HCV) is a single-stranded, positive-sense RNA ...an RNA-depen- dent RNA polymerase (RdRp) that is responsible for replica- tion of viral RNA ... See full document
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Selection of RNA Aptamers That Are Specific and High-Affinity Ligands of the Hepatitis C Virus RNA-Dependent RNA Polymerase
... NS5B⌬C21 polymerase (1, 26) the C-terminal residues 547 to 556 are buried in the putative template/primer-binding cleft, further occluding ...accessible RNA-binding cleft, thus ... See full document
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Sphingomyelin Activates Hepatitis C Virus RNA Polymerase in a Genotype-Specific Manner
... Hepatitis C virus (HCV) replication and infection depend on the lipid components of the cell, and replication is inhibited by inhibitors of sphingomyelin ...1b RNA-dependent RNA ... See full document
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Hydrophobic and Charged Residues in the C-Terminal Arm of Hepatitis C Virus RNA-Dependent RNA Polymerase Regulate Initiation and Elongation
... The RNA-dependent RNA polymerase (RdRp) of hepatitis C virus (HCV) is essential for viral genome ...two C-terminal features, a -loop and a C-terminal arm, ... See full document
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Biochemical properties of hepatitis C virus NS5B RNA-dependent RNA polymerase and identification of amino acid sequence motifs essential for enzymatic activity.
... for site- directed mutagenesis experiments, we identified four amino acid motifs which appear to play a pivotal role in polymerase ...NTP binding and catalysis and characterized by an ... See full document
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Oligomerization and Cooperative RNA Synthesis Activity of Hepatitis C Virus RNA-Dependent RNA Polymerase
... For Western blot analysis, yeast cell extracts were prepared by growing each strain in SC/Trp⫺Leu⫺ medium to mid-log phase and harvesting by centrifuga- tion. Cell pellets were washed with ice-cold breaking buffer ... See full document
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Structure of Hepatitis C Virus Polymerase in Complex with Primer-Template RNA
... HCV polymerase genotype 2a JFH-1 isolate was designed with the C-terminal 21 amino acids removed and replaced with a noncleavable hexahistidine tag, and it was cloned into a pET-28a-derived ...via ... See full document
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Template Requirements for RNA Synthesis by a Recombinant Hepatitis C Virus RNA-Dependent RNA Polymerase
... initiation site are different from some previous ...the C-terminal tail (19, 26). The C-ter- minal tail of NS5B is present in the active site of the crystal structure and has been hypothesized ... See full document
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Zinc Salts Block Hepatitis E Virus Replication by Inhibiting the Activity of Viral RNA-Dependent RNA Polymerase
... chronic hepatitis C cases, and topical zinc treatment has been used to control HSV infection (18, ...the binding of virus to the host cell surface and inhibit the activity of viral protease ... See full document
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