[PDF] Top 20 Mutational Analysis of the Herpes Simplex Virus Triplex Protein VP19C

... a protein sequence for functional do- ...of mutational analysis must be inter- preted with caution, particularly when considering the behavior of individual ...target protein or specific ... See full document

... Herpes simplex virus (HSV) virions contain one or more factors that trigger rapid shutoff of host protein synthesis and accelerated decay of cellular and viral mRNAs in infected ...vhs ... See full document

... with VP19C was seen by cryo-EM analysis of the B capsid ...high protein concentrations it formed a multimer ...with VP19C in the Y2H indicates that this protein transformation may not ... See full document

... immediate-early protein ICP0 (infected-cell polypeptide 0) of herpes simplex virus type 1 (HSV-1) is a promiscuous transactivator of both viral and nonviral promoters in transient expression ... See full document

... 6. Analysis of capsid-bound ...mutant virus were harvested at 18 h postinfection, layered onto 20 to 50% sucrose gradients, and centrifuged at 24,000 rpm (SW41 rotor) for 1 ...The protein composition ... See full document

... While it has been clear for a long time and has been reconfirmed in the accompanying report 11 that such assays of HSV promoter activity do not always accurately reflect kinetics or iden[r] ... See full document

... contrast, analysis of the protein products formed by plaque isolates 1737-14, 90-150-3, and 89-650-5, which con- tained the G insertion within the homopolymer stretch of 7 G residues, showed that a 28-kDa ... See full document

... Previous studies have suggested, but not proven, that the cellular target of REHAY-based peptides and, by extension, of the corresponding region of VP16 in VIC assembly, was HCF (13, 14, 49). However, an overlapping VP16 ... See full document

... Typical herpes simplex virus (HSV) capsids contain seven proteins that form a T ⴝ 16 icosahedron of 1,250-Å ...and VP19C (a component of the triplexes that connect adjacent capsomeres), ... See full document

... homologous protein of human cytomegalovirus (CMV), UL56 ...thermolabile protein with the other members of the ter- minase complex and viral growth at the nonpermissive tem- perature ... See full document

... the triplex as a largely uniform, globular mass with little evidence of separate domains which could be attributed to the two copies of VP23 and one of VP19C ...the triplex are more closely ... See full document

... The herpes simplex virus type 1 (HSV-1) UL25 gene encodes a minor capsid protein, pUL25, that is essential for packaging the full-length viral ...trace analysis to be important in ... See full document

... interactive protein, and this was seen by the absence of blue color in the colonies assayed (no-interaction columns in Tables 1 and ...the VP19C-VP23 interaction in this assay, that is, the kinetics of ... See full document

... Background: Herpes simplex virus (HSV) type 1 has a 152 kb double-stranded DNA genome that may encode more than 80 gene products, many of which remain ...HSV-1 triplex is a complex of three ... See full document

... HSV-1 protein kinase Us3 was tightly regu- lated by autophosphorylation of its serine 147 residue and that the regulation of Us3 activity by this autophosphorylation played a criti- cal role in HSV-1 replication ... See full document

... envelope protein folding ...aberrant protein folding of SU does not necessarily have to knock out efficient receptor binding, but it appears to have a major effect on the mechanism and efficiency of ... See full document

... baculo- virus-infected cells (4) was further purified by gel filtration chromatography (Superdex S200; GE Healthcare) using 10 mM Tris-HCl (pH ...purified protein was incubated with chymotrypsin at a ratio ... See full document

... Mouse LMtk- and human HEp-2 cell lines containing the gene for gD-l under control of the human metallothionein promoter II expressed various levels of gD-l constitutively and could be in[r] ... See full document

... as protein detected in WT transfected/infected cells ...of protein) as increasing amounts of the N terminus of ICP22 were ...the protein, this observation suggests that initiation of translation of U ... See full document

... a protein referred to as a replication ...simian virus 40 (SV40), whose ability to initiate viral DNA replication is both positively and negatively regulated by phosphorylation (reviewed in reference ...by ... See full document