[PDF] Top 20 Mutational analysis of the human immunodeficiency virus vpr open reading frame.
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Mutational analysis of the human immunodeficiency virus vpr open reading frame.
... RT activity was detected even 60 days after infection in i',f mutant-infected A3.01 cells with very weak CPE, whereas cells infected with vpr mutant or wild-type virus did not produce RT[r] ... See full document
5
Construction and genetic analysis of dicistronic polioviruses containing open reading frames for epitopes of human immunodeficiency virus type 1 gp120.
... , virus obtained from transfection displayed a large-plaque phenotype; 11 , virus from transfection had a mixed plaque phenotype; 1 , large plaques emerged after one or two additional virus ...RT-PCR ... See full document
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Efficiency of reinitiation of translation on human immunodeficiency virus type 1 mRNAs is determined by the length of the upstream open reading frame and by intercistronic distance.
... anemia virus (EIAV) bicistronic tat-rev mRNA, the initiator of the tat ORF is CUG, which allows for leaky scanning transla- tion and expression of the downstream rev ORF ... See full document
9
Human Herpesvirus 6 Open Reading Frame U83 Encodes a Functional Chemokine
... chemokine open reading frame (ORF), ORF U83, within the HHV-6 genome ...within virus genomes are able to produce proteins capable of inter- fering with the normal host defense response ... See full document
8
Deletion in Open Reading Frame 49 of Varicella-Zoster Virus Reduces Virus Growth in Human Malignant Melanoma Cells but Not in Human Embryonic Fibroblasts
... sequencing analysis, using primers for sequences up- stream and downstream of ...sequencing analysis with the primers for the upstream and downstream ORF49 sequences, to confirm the loss of ...blot ... See full document
12
Functional Analysis of the env Open Reading Frame in Human Endogenous Retrovirus IDDMK1,222 Encoding Superantigen Activity
... Since human T cells are stimulated in a V- specific pattern by MMTV-encoded Sags presented by human MHC-II-positive cells (17), and since the human and murine immune systems are very similar, it was ... See full document
8
Open reading frame vpr of simian immunodeficiency virus encodes a virion-associated protein.
... The vpr recombinant protein was partially purified by In addition to three structural genes, gag, pol, and env, which are commonly found in replication-competent animal retroviruses, hum[r] ... See full document
6
Molecular Analysis of Human Herpesvirus 8 by Using Single Nucleotide Polymorphisms in Open Reading Frame 26
... several open reading frames ...59 human immuno- deficiency virus-infected Japanese patients by using polymorphisms in ORF26 and found that over two-thirds of the HHV-8 isolates fell into major ... See full document
6
Mutational Analysis of the Human Immunodeficiency Virus Type 1 Vif Protein
... or vpr) into pIIIB; this made it possible to generate initial high-titer virus stocks in 293T cells (a permissive cell line) and then to use them to infect H9 cells at high efficiency and render them ... See full document
7
Incorporation of Vpr into human immunodeficiency virus type 1 virions: requirement for the p6 region of gag and mutational analysis.
... This effect did not appear to be due to transactivation of pcDNA-tag-Vpr by a factor encoded by the cotransfected provirus, since increased amounts of tagged Vpr were also present in lys[r] ... See full document
9
Mutational analysis of cell cycle arrest, nuclear localization and virion packaging of human immunodeficiency virus type 1 Vpr.
... that Vpr causes cell cycle arrest in trans- fected and infected cells (13, 32), it is not clear whether this activity is relevant to HIV-1 replication in monocytes/macro- phages or to AIDS ...of Vpr in the ... See full document
8
Identification and Prioritisation of Variants in the Short Open-Reading Frame Regions of the Human Genome
... the Human Gene Mutation Database (HGMD) (Stenson et al , 2014), which contains a list of inherited mutations that have been identified as pathogenic in peer-reviewed ... See full document
29
FAT10: a Novel Mediator of Vpr-Induced Apoptosis in Human Immunodeficiency Virus-Associated Nephropathy
... Previous studies have demonstrated that transduction of HK2 cells with HR-Vpr induces hypodiploidy and that hy- podiploidy was reflective of increased apoptosis as detected by annexin V and propidium iodide ... See full document
6
Interaction of the Human Immunodeficiency Virus Type 1 Vpr Protein with the Nuclear Pore Complex
... Dvpr virus replicated less well than the wild-type counter- part—even though the absolute values for the magnitudes of replication varied ...Dvpr virus (including numerous additional experiments that are ... See full document
10
Biochemical Analyses of the Interactions between Human Immunodeficiency Virus Type 1 Vpr and p6Gag
... Human immunodeficiency virus type 1 (HIV-1) encodes a 97-amino-acid accessory protein called Vpr (an initialism for viral protein ...Although Vpr is dispensable for HIV-1 growth in ... See full document
6
Human Immunodeficiency Virus Type 1 Vpr Links Proteasomal Degradation and Checkpoint Activation
... mutated and Rad3-related protein (ATR). ATR is a sensor of replication stress. Replication stress is a cellular condition that results from the stalling of replication forks as a consequence of various cellular insults, ... See full document
7
Peptidomic discovery of short open reading frame-encoded peptides in human cells
... In contrast to previous attempts to use mass spectrometry to discover unannotated human coding sequences, we successfully access the pool of SEPs that are under 50 amino acids in length. This is unprecedented for ... See full document
21
Mutation in the UL97 open reading frame of human cytomegalovirus strains resistant to ganciclovir.
... Although the amino acid sequence indicates that the UL97 product may have protein kinase or phosphotransferase activity, Sullivan and coworkers 31 identified this open reading frame as t[r] ... See full document
5
Human Immunodeficiency Virus Type 1 Vpr Induces Apoptosis through Caspase Activation
... that Vpr interacts with a compo- nent(s) within the cell that results in activation of the caspase cascade either directly or ...against Vpr-induced apoptosis ...and analysis of Vpr-induced ... See full document
7
Phosphorylation of Human Immunodeficiency Virus Type 1 Vpr Regulates Cell Cycle Arrest
... Cell lines and DNA transfection. HEK293, 293T, BSC40, MAGI, and MAGI5 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supple- mented with 10% heat-inactivated fetal calf serum (FCS), 100 U of ... See full document
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