[PDF] Top 20 Nucleolin Is Required for Efficient Nuclear Egress of Herpes Simplex Virus Type 1 Nucleocapsids
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Nucleolin Is Required for Efficient Nuclear Egress of Herpes Simplex Virus Type 1 Nucleocapsids
... and virus infection (9, 34, ...of nucleolin may be involved in regulation of HSV-1 nuclear ...that nuclear domain(s) in which nucleolin concentrated was marginalized and detected ... See full document
12
The UL14 Tegument Protein of Herpes Simplex Virus Type 1 Is Required for Efficient Nuclear Transport of the Alpha Transinducing Factor VP16 and Viral Capsids
... cell-associated virus stocks starting at an MOI of 1 PFU/cell was submitted to SDS-PAGE and Western blotting, and examined for VP16-GFP with anti-GFP ... See full document
13
Nuclear export of VP19C is not essential for replication of herpes simplex virus type 1
... and efficient modification of viral genomes in ...the virus life cycle and pathogenesis ...ant virus using BAC recombineering technology. The recombinant virus with the NES mutation in VP19C ... See full document
5
Genetic Analysis of the Role of Herpes Simplex Virus Type 1 Glycoprotein K in Infectious Virus Production and Egress
... mutant virus indicated that the carboxyl terminus of gK (do- main IV) is not required for virus replication and virus ...to nuclear membranes as well as throughout the ...outer ... See full document
12
Herpes simplex virus type 1 alkaline nuclease is required for efficient processing of viral DNA replication intermediates.
... of herpes simplex virus type 1 (HSV-1) (nuc mutations) induce almost wild-type levels of viral DNA; however, mutant viral yields are ...to 1% of wild-type ... See full document
11
The Torsin Activator LULL1 Is Required for Efficient Growth of Herpes Simplex Virus 1
... in nuclear envelope dynamics as well as the nuclear egress of herpes simplex virus 1 ...LULL1, type II transmembrane proteins that are integral parts of the ... See full document
9
DNA Mismatch Repair Proteins Are Required for Efficient Herpes Simplex Virus 1 Replication
... either 1% Triton X-100 in PBS (Fix/Perm type 1) or with ice-cold acetone for 2 min at room temperature (Fix/Perm type ...a nuclear counterstain. Alexa Fluor secondary antibodies ... See full document
13
Role of Herpes Simplex Virus 1 Immediate Early Protein ICP22 in Viral Nuclear Egress
... the nuclear capsids is signifi- cantly ...viral egress from the nucleoplasm decreases in the absence of ICP22 compared to that in the presence of ICP22, but the rate of egress from the perinuclear ... See full document
10
Herpes Simplex Virus Type 1 UL51 Protein Is Involved in Maturation and Egress of Virus Particles
... enveloped nucleocapsids was frequently observed. Nuclear vacuoles harboring HSV-1 par- ticles have been reported and are believed to be continuous with the perinuclear cisterna, resulting from the ... See full document
10
Role of Host Cell p32 in Herpes Simplex Virus 1 De-Envelopment during Viral Nuclear Egress
... the herpes simplex virus 1 (HSV-1) major virion structural protein UL47 (also designated VP13/14), we screened cells overexpressing UL47 for UL47-binding cellular ...the nuclear ... See full document
17
Evidence that Herpes Simplex Virus VP16 Is Required for Viral Egress Downstream of the Initial Envelopment Event
... and nuclear membranes, fails to reach the Golgi apparatus and cell surface, and is not incorporated into HSV-1 virions ...in efficient virion envelopment and transloca- tion to the extracellular ... See full document
13
Herpes Simplex Virus 1 Induces Phosphorylation and Reorganization of Lamin A/C through the γ134.5 Protein That Facilitates Nuclear Egress
... nuclear egress. Next, we examined nuclear egress by transmission electron ...to nucleocapsids. R3616 infection produced virus particles that were mainly in the nucleus ... See full document
9
Herpes Simplex Virus Type 1 UL34 Gene Product Is Required for Viral Envelopment
... for efficient envelopment at the inner nuclear membrane have been ...wild-type virus-infected cells, empty capsids are only rarely enveloped (35), and a variety of viral mutants that ... See full document
13
Pocket Protein p130/Rb2 Is Required for Efficient Herpes Simplex Virus Type 1 Gene Expression and Viral Replication
... globular nuclear ICP8 staining pattern was observed in almost 100% of cells at 5 h ...diffuse nuclear ICP8 staining was observed ...than 1% of p130 ⫺/⫺ cells ... See full document
12
Herpes Simplex Virus Type 1 Immediate-Early Protein ICP27 Is Required for Efficient Incorporation of ICP0 and ICP4 into Virions
... strong nuclear localization and a smaller amount of cytoplasmic ...predominantly nuclear, whereas much more of the d3-4 ICP27 was located in the ...in nuclear localization (26, ...strong ... See full document
10
Arginine-rich regions succeeding the nuclear localization region of the herpes simplex virus type 1 regulatory protein ICP27 are required for efficient nuclear localization and late gene expression.
... (nt) 1 and the SstI site designated nt 2421 as previously described (23), were digested at the BamHI site and the EcoRI site which occurs in the PEFR linker present in both ...residues 1 to 103, was joined ... See full document
12
Nucleolin Interacts with US11 Protein of Herpes Simplex Virus 1 and Is Involved in Its Trafficking
... of nucleolin prevents HSV-1 infec- tion (6), and the expression of nucleolin is required for nuclear egress of HSV-1 nucleocapsids (32), demonstrating that ... See full document
9
Herpes Simplex Virus 1 UL47 Interacts with Viral Nuclear Egress Factors UL31, UL34, and Us3 and Regulates Viral Nuclear Egress
... this type of virion was substan- tially ...of nuclear capsids increased. The accumulation of nuclear capsids and the lack of primary enveloped virions in the perinuclear space in the absence of UL47 ... See full document
11
Ultrastructural Localization of the Herpes Simplex Virus Type 1 UL31, UL34, and US3 Proteins Suggests Specific Roles in Primary Envelopment and Egress of Nucleocapsids
... (VZV) and pseudorabies virus (PrV), and betaherpesviruses such as human cytomegalovirus. In this model, primary envel- opment occurs by budding through the INM but the primary envelope surrounding the perinuclear ... See full document
14
Nucleolin Is Required for an Efficient Herpes Simplex Virus Type 1 Infection
... replication, virus assembly, and control of intracellular trafficking (for reviews see references 22 and ...protein nucleolin (65). Nucleolin is one of the most abun- dant nonribosomal proteins of ... See full document
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