[PDF] Top 20 Nucleotide sequence of the inverted terminal repetition in adeno-associated virus DNA.
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Nucleotide sequence of the inverted terminal repetition in adeno-associated virus DNA.
... Structural studies of the AAV genome demonstrated the existence of an inverted terminal nucleotide sequence repetition 17 which also had the properties of a natural terminal repetition; [r] ... See full document
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Evidence for two nucleotide sequence orientations within the terminal repetition of adeno-associated virus DNA.
... Because the terminal cleavage site of Hpa II is within the inverted terminal repetition, only a single species of terminal fragment i.e., the same from both ends would be expected if dou[r] ... See full document
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Characterization of Adenovirus-Induced Inverted Terminal Repeat-Independent Amplification of Integrated Adeno-Associated Virus rep-cap Sequences
... In this study, we further investigated the mechanisms under- lying rep-cap amplification. First, by comparing different stable rep-cap cell lines, we found that among the various cell back- grounds examined, rep-cap ... See full document
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DNA-Binding Activity of Adeno-Associated Virus Rep Is Required for Inverted Terminal Repeat-Dependent Complex Formation with Herpes Simplex Virus ICP8
... helper virus, the plasmids mediated comparable Rep expression but, with the exception of Rep78wt, had lost DNA replication properties ...the DNA binding- deficient mutants RepR107A/K136A and ... See full document
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Interaction of the adeno-associated virus Rep protein with a sequence within the A palindrome of the viral terminal repeat.
... Since the pattern of Rep-DNA complexes was not significantly different when the A stem or TR was used as a substrate, it is likely that the 10:1 ratio found for the A stem reflects the s[r] ... See full document
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Role of the Adenovirus DNA-Binding Protein in In Vitro Adeno-Associated Virus DNA Replication
... Ad DNA replication, the DNA polymerase and the terminal pro- tein make no contribution to AAV DNA ...Ad DNA-binding protein (Ad-DBP) have been ambig- uous (reviewed in reference ...AAV ... See full document
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Adeno-associated virus Rep78 protein and terminal repeats enhance integration of DNA sequences into the cellular genome.
... line DNA, thus ruling out the possibility of a cross-hybridization with human-related se- ...any sequence contained between the two EcoRI sites in plasmid GT9012 (depicted in ...genomic DNA from ... See full document
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Efficient Integration of Recombinant Adeno-Associated Virus DNA Vectors Requires a p5-rep Sequence in cis
... of adeno-associated virus (AAV) and adenovirus (Ad) gene therapy vectors to generate an Ad-AAV hybrid vector allowing efficient site-specific integration with Ad ...enhancer sequence element ... See full document
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A novel 165-base-pair terminal repeat sequence is the sole cis requirement for the adeno-associated virus life cycle.
... Adeno-associated virus (AAV) replication is dependent on two copies of a 145-bp inverted terminal repeat (ITR) that flank the AAV ...AAV sequence: two copies of the D element, a ... See full document
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Inverted Terminal Repeat Sequences Are Important for Intermolecular Recombination and Circularization of Adeno-Associated Virus Genomes
... AV2:5/2eGFP virus had a substantially lower ability to form circular intermediates than the AV2:2/2eGFP and AV5:5/2eGFP ...ITR virus were ...each virus alone or in combination, as ...assess ... See full document
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Adeno-associated virus type 2 DNA replication in vivo: mutation analyses of the D sequence in viral inverted terminal repeats.
... the virus life ...A sequence and the D sequence, ...D sequence led to destruction of the functional trs, resulting in impaired rescue and replication of the AAV ...D sequence, ... See full document
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A novel terminal resolution-like site in the adeno-associated virus type 2 genome.
... AAV DNA replica- tive ...vector DNA could be readily detected even when the plasmid DNA was not digested with XbaI prior to ...vector DNA also occurred only from plasmid pXS-30 with prior ... See full document
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Identification of nuclear proteins that specifically interact with adeno-associated virus type 2 inverted terminal repeat hairpin DNA.
... Indeed, specific proteins were detected in nuclear extracts prepared from Ad2-AAV-coinfected human KB cells that interacted with the AAV hairpin DNA but not with the double-stranded dupl[r] ... See full document
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Sequence requirements for binding of Rep68 to the adeno-associated virus terminal repeats.
... RBE sequence contains a set of four imperfect GAGC motifs which we and others (1, 10, 22, 23, 35) had speculated might be the important feature of this element for Rep ...the sequence recog- nized by Rep ... See full document
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Transcriptional and translational mapping of a 6.6-kilobase-pair DNA fragment containing the junction of the terminal repetition and unique sequence at the left end of the vaccinia virus genome.
... Hybridization selection and cell-free translation of mRNA's encoded within the inverted terminal repetition of the vaccinia virus genome.. Extension of the transcriptional and translatio[r] ... See full document
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Sequence requirements for stable binding and function of Rep68 on the adeno-associated virus type 2 inverted terminal repeats.
... This report demonstrates the ability of both recombinant fusion maltose-binding protein MBP-Rep68A produced in Escherichia coli and wild-type wt Rep68 to bind to a linear truncated form [r] ... See full document
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Physical mapping of adeno-associated virus serotype 4 DNA.
... both enzymes produce a 320-base pair terminal fragment and is analyzed on 3 to 15% polyacrylamide gradient gels, submolar bands, which appear to represent the smaller terminal fragment, [r] ... See full document
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Effects of Adeno-Associated Virus DNA Hairpin Structure on Recombination
... wt terminal repeats ...of DNA recombination were constructed with the intact expression cassette (including the exogenous intron) cloned in each orientation with respect to the viral open and closed TR ... See full document
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Nucleotide sequence required for resolution of the concatemer junction of vaccinia virus DNA.
... The sequence shown to be required for the resolution of plasmids containing the vaccinia virus concatemer junction was compared with the region of the genome proximal to the hairpin loop[r] ... See full document
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Nucleotide sequence of the region encompassing the JC virus origin of DNA replication.
... Similarities include palindromic and symmetrical sequences near their origins of DNA replication, binding sites for their large T proteins, an AT-rich region the Goldberg-Hogness, or TAT[r] ... See full document
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