[PDF] Top 20 Oligomerization and origin DNA-binding activity of simian virus 40 large T antigen.
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Oligomerization and origin DNA-binding activity of simian virus 40 large T antigen.
... In contrast, studies carried out with lytic T-antigen subclasses separated by zone velocity sedimentation or phosphocellulose chromatography showed that newly synthesized 5 to 7S T antig[r] ... See full document
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Functional implications of oligomerization of simian virus 40 large T antigen during lytic virus infection.
... To determine the distribution of various forms of T antigen on the sucrose density gradients at a quantitative level, the corresponding gel bands were excised, eluted with 0.2 ml of 60% [r] ... See full document
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Oligomerization of simian virus 40 large T antigen is not necessarily repressed by temperature-sensitive A gene lesions.
... tsA1499-infected cells were kept for 96 h at 32 or 41°C, radiolabeled with [32P]phosphate, extracted, and analyzed for both T-p53 complexes and free T antigen by sequential immunoprecipi[r] ... See full document
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The N-terminal side of the origin-binding domain of simian virus 40 large T antigen is involved in A/T untwisting.
... of simian virus 40 (SV40) large T antigen’s origin-binding domain in the initiation of virus DNA replication by analyzing the biochemical activities of ... See full document
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Quantitative Analysis of the Binding of Simian Virus 40 Large T Antigen to DNA
... upon binding to nonspecific ds- and ssDNA in FP ...on DNA is related to spiral formation is un- ...another T-ag molecule, albeit at high concentrations, as would be the case for two T-ag ... See full document
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Purification of the simian virus 40 (SV40) T antigen DNA-binding domain and characterization of its interactions with the SV40 origin.
... free DNA or protein-DNA complexes, were excised and eluted overnight at 378C in 550 ml of elution buffer ...centrifugation, DNA-containing solutions were subjected to extractions with ... See full document
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Three domains in the simian virus 40 core origin orchestrate the binding, melting, and DNA helicase activities of T antigen.
... Simian virus 40 large tumor antigen requires three core replication origin domains for DNA unwinding and replication in vitro.. Simian virus 40 SV40 DNA replication: SV40 large T antigen[r] ... See full document
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Sequence Requirements for the Assembly of Simian Virus 40 T Antigen and the T-Antigen Origin Binding Domain on the Viral Core Origin of Replication
... for T-ag assembly events, we analyzed binding to larger subfragments of the core ...support T-ag assem- bly, albeit at lower levels compared to wild-type sequences, indicates that both ... See full document
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Dimerization of simian virus 40 T-antigen hexamers activates T-antigen DNA helicase activity.
... comparing T-antigen binding to the DNA fork and DNA bubble, reaction mixtures containing ...the DNA fork or bubble substrates, 700 ng of T antigen, ...duplex ... See full document
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Simian virus 40 origin DNA-binding domain on large T antigen.
... The variants used to map the DNA-binding domain include: i fragments of large T produced by in vitro translation of unspliced SV40 cRNA 42; ii various truncated forms of large T present [r] ... See full document
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Simian Virus 40 Large T Antigen Can Specifically Unwind the Central Palindrome at the Origin of DNA Replication
... of simian virus 40 (SV40) large T antigen play a critical role in DNA ...normal DNA binding and ATPase and helicase activities but with a severely reduced ... See full document
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Identification of simian virus 40 T-antigen residues important for specific and nonspecific binding to DNA and for helicase activity.
... 4858 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest We have previously identified three regions called elements in the DNA-binding domain of simian virus 40 large tumo[r] ... See full document
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Expression of simian virus 40 T antigen in Escherichia coli: localization of T-antigen origin DNA-binding domain to within 129 amino acids.
... However, a description of the precise protein-DNA interactions of T antigen with these two different sites at a level comparable to that of procaryotic DNA-binding proteins 33 requires m[r] ... See full document
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Simian virus 40 small t antigen activates the carboxyl-terminal transforming p53-binding domain of large T antigen.
... various DNA tumor viruses generally is considered as a means to inactivate functions of these proteins in cell cycle control (27, 42, 43, ...SV40 large T with p53 might provide a good example for ... See full document
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Four major sequence elements of simian virus 40 large T antigen coordinate its specific and nonspecific DNA binding.
... By testing the effects of single-site mutations on virus replication and by performing a number of DNA-binding assays on replication-negative T-antigen mutants, we have identified severa[r] ... See full document
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Simian Virus 40 Large T Antigen Disrupts Genome Integrity and Activates a DNA Damage Response via Bub1 Binding
... pRB binding was required, since other viral onco- proteins have been shown to induce the DDR via this pathway ...pRB binding deficient (via a K1 mutation) ...lar DNA replication (4, 17, ...Bub1 ... See full document
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Effects of in vitro dephosphorylation on DNA-binding and DNA helicase activities of simian virus 40 large tumor antigen.
... 22 showed that dephosphorylation by alkaline phosphatase increases the affinity of T antigen to the SV40 binding site II and, furthermore, that the activity of dephosphorylated T antigen[r] ... See full document
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Structure-Based Analysis of the Interaction between the Simian Virus 40 T-Antigen Origin Binding Domain and Single-Stranded DNA
... the T-Ag OBD and its interactions with ...the T-Ag OBD by nuclear magnetic resonance (NMR) meth- ods (30) and established that it is a monomer in ...the T-Ag OBD bound in a site-specific manner to a ... See full document
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Allosteric control of simian virus 40 T-antigen binding to viral origin DNA.
... Nucleotide inhibition of origin DNA binding is observed in three different DNA-binding assays with immunopurified COS1 T antigen, biochemically purified lytic T antigen, and crude COSi c[r] ... See full document
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Characterization of the DNA-Binding Properties of the Origin-Binding Domain of Simian Virus 40 Large T Antigen by Fluorescence Anisotropy
... detect binding of full-length T antigen to fluorescent probes under the same salt ...D, T antigen was able to bind to the TBS-containing probe in a sequence-specific manner but with a ... See full document
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