[PDF] Top 20 Predicting the sensitivity and specificity of published real-time PCR assays
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Predicting the sensitivity and specificity of published real-time PCR assays
... available real time PCR analysis tool called TaqSim [4] was used to find public sequences that would match the primer/probe assay in ...for specificity by requir- ing that hits have fewer than ... See full document
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Monitoring of leukocyte cytomegalovirus DNA in bone marrow transplant recipients by nested PCR
... nested PCR assay for the detection of human cytomegalovirus (CMV) DNA was evaluated by weekly monitoring of blood samples taken from 101 bone marrow transplant (BMT) ...The sensitivity, specificity, ... See full document
5
Comparative evaluation of published real time PCR assays for the detection of malaria following MIQE guidelines
... The WHO International Standard for Plasmodium falcip- arum DNA was used to analyse the efficiency, sensitivity and specificity of published assay targets for Plasmodium spp . and P. falciparum . The ... See full document
8
Real Time PCR for Detection and Identification of Plasmodium spp
... chromatographic assays based on antigen detection have been developed but are also relatively insensitive in cases of low parasitemia (22, 24, ...using PCR has resulted in increased sensitivity and ... See full document
6
Multiplex Real Time PCR for Detection of Anaplasma phagocytophilum and Borrelia burgdorferi
... multiplex real-time PCR assay was developed for the simultaneous detection of Anaplasma phagocytophilum and Borrelia ...analytical sensitivity of the assay is comparable to that of previously ... See full document
5
Evaluation of Twelve Real Time Reverse Transcriptase PCR Primer Probe Sets for Detection of Pandemic Influenza A/H1N1 2009 Virus
... transcriptase PCR (rRT-PCR) assays have greatly contributed to the detection, control, and prevention of the pandemic influenza A/H1N1 2009 ...rRT-PCR assays for detection of pandemic ... See full document
7
Rapid Stool Based Diagnosis of Clostridium difficile Infection by Real Time PCR in a Children's Hospital
... cytotoxicity assays has been largely replaced by rapid enzyme immunoassays ...of sensitivity of stool EIA, we developed a multiplex real-time PCR assay targeting the ...and ... See full document
7
Detection of group a streptococcal pharyngitis by quantitative PCR
... good specificity (>95%), they often have reduced sensitivity (~85%) compared to culture ...good specificity (>95%) [15,16]. Real-time quantitative PCR (qPCR) assays ... See full document
7
Optimization of a Combined Human Parechovirus Enterovirus Real Time Reverse Transcription PCR Assay and Evaluation of a New Parechovirus 3 Specific Assay for Cerebrospinal Fluid Specimen Testing
... good sensitivity except for some strains of E6, where a C-to-U difference at the 3= end of the forward primer was observed ...the published data, as the assay with our E6 strain (ATCC VR-36; D’Amori) had a ... See full document
7
Clinical Validation of Multiplex Real Time PCR Assays for Detection of Bacterial Meningitis Pathogens
... Real-time PCR is a powerful tool for detection of human patho- gens because of the high sensitivity and throughput ...assay specificity and enables development of a multiplex platform ... See full document
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Performances of Four Real Time PCR Assays for Diagnosis of Pneumocystis jirovecii Pneumonia
... Classification of the probability of PCP. Clinical, radiological, and treatment data were collected retrospectively for all patients included. The clinical history of each patient was analyzed by two specialists (a ... See full document
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Beckman Access versus the Bayer ACS:180 and the Abbott AxSYM cardiac Troponin-I real-time immunoassays: an observational prospective study
... in real-time analysis ...in real- time clinical trials. Additionally, under real-time labora- tory conditions, machines running multiple analytes in random-access mode, may be ... See full document
8
Comparison and Recommendations for Use of Dientamoeba fragilis Real Time PCR Assays
... of real-time PCR methodologies for the detection of ...of real-time PCR are well publicized; consequently, real-time PCR has become the gold standard for ... See full document
10
Development and Validation of a Quantitative PCR Assay for Diagnosis of Scedosporiosis
... prolificans. PCR products were subjected to electrophoresis in 2% agarose gels (Prona- disa, Madrid, Spain) following the protocols of Sambrook et ...Each PCR run contained both negative and positive ... See full document
5
Comparative Evaluation of Real Time PCR Assays for Detection of the Human Metapneumovirus
... immunofluorescent assays for paramyxoviruses (hRSV, parainfluenza viruses 1 to 3) and other respiratory viruses (adenoviruses, influenza viruses A and B); and (iii) no hemadsorption of human red blood ...be ... See full document
5
Real time PCR assays for hepatitis B virus DNA quantification may require two different targets
... Group A consisted of 104 plasma samples obtained from patients at the 302 Military Hospital of China and group B consisted of 59 plasma samples obtained from patients at Beijing ChaoYang Hospital between July 2016 and ... See full document
9
External Quality Assessment for Enterovirus 71 and Coxsackievirus A16 Detection by Reverse Transcription PCR Using Armored RNA as a Virus Surrogate
... commercial real-time RT-PCR kits (PCR assays A to E), one triplex commercial real-time RT- PCR assay (PCR assay F), and one in-house conventional RT- ... See full document
5
Comparison of Real Time PCR Assays with Fluorescent Antibody Assays for Diagnosis of Respiratory Virus Infections in Children
... quantitative PCR methods and including clinical data are being performed to determine what AdV viral load is considered significant for a pathogenic role in respiratory ...by PCR for all patient age ...by ... See full document
7
Development of Conventional and Real Time Quantitative PCR Assays for Diagnosis and Monitoring of Scabies
... For patients with crusted scabies, skin swab specimens were addition- ally taken at the same time that the skin scrapings were taken for PCR. Swab specimens were taken from the six body sites which are most ... See full document
8
Quantitative PCR Assessments of Bacterial Species in Women with and without Bacterial Vaginosis
... Study population and sample collection/selection. Vaginal swab samples for this study were collected over the course of several years (2002 to 2004) in the New Orleans sexually transmitted diseases (STD) clinic following ... See full document
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