[PDF] Top 20 Quantitative Analysis of Epstein Barr Virus Load by Using a Real Time PCR Assay

... 35). Using this system, it is possible to analyze more than 40 samples in 2 to 3 h even if they are tested in ...EBV load in patients with symptomatic EBV ...tected PCR inhibitors and estimated the ... See full document

... by PCR in KS lesions, peripheral blood mononuclear cells (PBMCs), and different types of samples ...viral load in PBMCs and KS plaques is correlated with the spread and gravity of these lesions ...ficiency ... See full document

... of assay design, relevant issues include probe chemistry, target selection, cycling conditions, internal control (selection of tar- get and mode of use), thermocycling platform, and quantitative calibration ... See full document

... curves PCR products of HAdV were obtained with the two step nested PCR as described in previous study ...the quantitative analysis of tq- ... See full document

... TaqMan PCR (Applied Biosystems) for EBV DNA load quantification in unfractionated whole- blood, using the method exactly as described recently ...This assay gave good results when plasmid DNA ... See full document

... between Epstein-Barr virus (EBV) DNA load and clinical course of patients with chronic lymphocytic leukemia (CLL) is ...DNA load by quantitative PCR at CLL presentation in ... See full document

... ever, the LightCycler EBV quantification kit also showed more scattered results, with a range of concentrations from 4,821 copies/ml to 1,494,000 copies/ml. The highest viral loads were found in the groups of CNS ... See full document

... related virus, JCV, for which a significant number of sequences were available, proved to be useful in substantiating that the ...nucleotide analysis of BKV isolates ... See full document

... purposes, using only their routine testing ...and quantitative calibrators and on the type of calibration material (whole organism quantified by various means or synthetic oligonucleotide) ...intact ... See full document

... simplex virus 1 (HSV-1) esophagitis diagnosis is routinely based on the endoscopic findings confirmed by histopatholog- ical examination of the esophagitis ...qualitative PCR assay from esophagitis ... See full document

... Monitoring of EBV, CMV, and HHV-6 DNA loads in a transplant recipient. Figure 5 shows changes in the viral DNA loads in a representative case. The patient was a 4-year-old girl with severe aplastic anemia who underwent ... See full document

... of real time PCR, the emphasis of the initial studies [12,14,16,17] on detecting EBV now includes determination of viral ...study, quantitative real time PCR (QPCR) was ... See full document

... TaqMan-based real- time RT-PCR has been shown to be a rapid, sensitive, and specific assay for the detection of RSV A and B in ...a real-time PCR assay in immuno- ... See full document

... by real-time PCR were performed as described previously using primers and a probe located within the EBNA1 gene and the HHV-6 U57 gene, respectively ...TaqMan PCR was carried out with a ... See full document

... chips using the Quantstudio 3D Digital PCR chip loader in a mixture consisting of 2X Quantstudio 3D Digital PCR master mix and 20X HBV Taqman assay (Pa_03453405) as mentioned in the digital ... See full document

... plex virus type 1 (HSV-1) and HSV-2 DNA were tested for specific ...sorting using a B-cell isolation kit (Miltenyi Biotec, Bergisch Gladbach, ...sorter analysis revealed a median purity of ...the ... See full document

... EBV load. This method, however, is laborious, time-consuming, and not suitable for large-scale screening of patient ...above, quantitative PCRs (Q-PCRs), using competitive coamplifica- tion of ... See full document

... Sixty nine cases of NK/T cell lymphomas were collected from the Department of Pathology of Affiliated Hospital of Qingdao University between January 2009 and December 2012. The study was approved by the Medical Ethics ... See full document

... The SROCs based on specificity and sensitivity are shown in Figure 6. The AUC was 0.92 for tissue and 0.71 for brush- ing samples (tissue: AUC = 0. 92, 95% CI [0.90–0.94], Figure 6A; brushing: AUC = 0.71, 95% CI [0.67, ... See full document

... set. Using these criteria, we identified eight house- keeping genes PSMD2, PSMB3, TCLF1, PTDSS1, AAMP, ACTB, c-yes, and HMBS for the normalization housekeep- ing gene set (Table ... See full document