[PDF] Top 20 Real Time PCR for detection of herpes simplex virus without nucleic acid extraction
Has 10000 "Real Time PCR for detection of herpes simplex virus without nucleic acid extraction" found on our website. Below are the top 20 most common "Real Time PCR for detection of herpes simplex virus without nucleic acid extraction".
Real Time PCR for detection of herpes simplex virus without nucleic acid extraction
... of real-time polymerase chain reaction (PCR) have made it a popular method for the detection of microbiological agents in both research and clinical ...the detection and genotyping of ... See full document
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Effect of Sequence Polymorphisms on Performance of Two Real Time PCR Assays for Detection of Herpes Simplex Virus
... tiplexed, real-time assay for the detection of HSV-1 and -2, using a novel type of hybridization probe termed the Eclipse probe (Epoch Biosciences) (1, ...monitor nucleic acid ... See full document
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Development and Validation of a Laboratory Developed Multiplex Real Time PCR Assay on the BD Max System for Detection of Herpes Simplex Virus and Varicella Zoster Virus DNA in Various Clinical Specimens
... multiplex real-time PCR (quantitative PCR [qPCR]) assay detecting herpes simplex virus (HSV) and varicella-zoster virus (VZV) DNA together with an internal control ... See full document
6
Detection of Herpes Simplex Virus DNA by Real Time PCR
... more prone to contamination than the LC assay. In this study, however, contaminations may have occurred with both assays. The major weaknesses in assay design that could lead to false- positives include sample ... See full document
5
Detection of Methicillin Resistant Staphylococcus aureus and Simultaneous Confirmation by Automated Nucleic Acid Extraction and Real Time PCR
... simultaneous detection of a Staphylococcus aureus-specific gene and the mecA gene, responsible for the resistance to methicillin in staphylococci, was ...DNA extraction protocol conducted with a MagNA Pure ... See full document
6
Detection, quantification and genotyping of Herpes Simplex Virus in cervicovaginal secretions by real time PCR: a cross sectional survey
... We found anti HSV-2 IgG seropositivity correlated poorly with HSV-2 secretion and several factors may have con- tributed to this observation. IgG seropositivity may take time to develop and the Kalon antibody test ... See full document
10
Increased Detection Rate in Diagnosis of Herpes Simplex Virus Type 2 Meningitis by Real Time PCR Using Cerebrospinal Fluid Samples
... of virus infections in the central nervous ...of PCR methods for early diagnosis of meningitis due to herpes simplex type 2 (HSV-2) and varicella-zoster virus ...evaluate ... See full document
5
Virological Diagnosis of Herpes Simplex Virus 1 Esophagitis by Quantitative Real Time PCR Assay
... performing PCR with paraffin-embedded tissues, where many inhibitors are encountered, which could lead to false-negative PCR ...the real-time PCR assay showed a low positive predictive ... See full document
5
Comparison of Specimen Processing and Nucleic Acid Extraction by the Swab Extraction Tube System versus the MagNA Pure LC System for Laboratory Diagnosis of Herpes Simplex Virus Infections by LightCycler PCR
... for nucleic acid extraction. Thus, for MagNA Pure LC extraction, one of two dual swabs was initially swirled in 2 ml of serum-free medium prior to real-time PCR ampli- ... See full document
5
Evaluation of Three Automated Nucleic Acid Extraction Systems for Identification of Respiratory Viruses in Clinical Specimens by Multiplex Real-time PCR
... RV16 Detection kit (Seegene, Republic of ...The nucleic acids extracted by EZ1 Advanced XL showed higher positive rates for virus detection than the ...from nucleic extraction to ... See full document
9
Real Time PCR Quantification of Genital Shedding of Herpes Simplex Virus (HSV) and Human Immunodeficiency Virus (HIV) in Women Coinfected with HSV and HIV
... laboratory-developed real-time PCR procedures using a Light Cycler instrument (Roche Diagnostics) for detecting and quantifying human immunodeficiency virus type 1 (HIV-1) RNA and DNA as well ... See full document
10
Detection of Herpes Simplex Virus Infection in Cerebrospinal Fluid
... routine real-time PCR test is suggested for HSV detection in patients with encephalitis to avoid unnecessary antiviral ...Encephalitis, Herpes simplex virus, ... See full document
5
Analysis of Individual Human Trigeminal Ganglia for Latent Herpes Simplex Virus Type 1 and Varicella-Zoster Virus Nucleic Acids Using Real-Time PCR
... limited virus reactivation. However, the time separating death and autopsy was not correlated with the VZV DNA copy number in the ...average time between death and au- topsy (tissue removal and flash ... See full document
8
A real time RT PCR for detection of clade 1 and 2 H5N1 Influenza A virus using Locked Nucleic Acid (LNA) TaqMan probes
... the detection of influenza A of subtype ...both virus isolates and human clinical specimens. Cross-reaction with virus iso- lates from other influenza A subtypes was not observed, and no positive ... See full document
5
Simultaneous Detection and Differentiation of Human Rhino and Enteroviruses in Clinical Specimens by Real Time PCR with Locked Nucleic Acid Probes
... cific detection of these viruses is reverse transcription (RT)-PCR with primers targeting the conserved 5= noncoding region of the viral ...differential detection. In this study, we describe the use ... See full document
8
Comparison of Herpes Simplex Virus PCR with Culture for Virus Detection in Multisource Surface Swab Specimens from Neonates
... recommends herpes sim- plex virus (HSV) culture or PCR for testing of swabs of the conjunctivae, mouth, na- sopharynx, and rectum (surface swabs) from ...and time to results of HSV PCR ... See full document
5
Quantitative Detection of Hepatitis B Virus DNA by Real Time Nucleic Acid Sequence Based Amplification with Molecular Beacon Detection
... sample extraction method, and template denaturation, the NASBA technique can be made suitable for DNA target amplification resulting in RNA ...for nucleic acid detection. The homogeneous ... See full document
10
Use of the MagNA Pure LC Automated Nucleic Acid Extraction System followed by Real Time Reverse Transcription PCR for Ultrasensitive Quantitation of Hepatitis C Virus RNA
... ⫽ 0.871). Approximately 77% of specimens showed agreement of viral load within 0.5 log, as determined by the real-time RT-PCR versus bDNA methods, and approximately 95% agreed within 1 log. We also ... See full document
7
Characterization of Virus Isolates by Particle Associated Nucleic Acid PCR
... Diagnostic virus isolation is still frequently used, particularly from respiratory tract ...positive virus cultures for all possible viruses is time-consuming, and unexpected or unknown viruses may ... See full document
5
Original Article An allele specific locked nucleic acid real time quantitative PCR for detection of HBV rtA181V and rtN236T mutations
... B virus (HBV) are the major causes for unsuccessful therapy for chronic hepatitis B infection ...Early detection of ADV-resistant mutationsis of great clinical ...approach, real time allele ... See full document
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