[PDF] Top 20 Replication Stress and Mitotic Dysfunction in Cells Expressing Simian Virus 40 Large T Antigen
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Replication Stress and Mitotic Dysfunction in Cells Expressing Simian Virus 40 Large T Antigen
... reflects mitotic dysfunction, and it binds the mitotic check- point kinase Bub1, we investigated whether LT affects mitotic pro- ...BJ-V cells were engineered to express histone H2B-GFP ... See full document
14
Replication of simian virus 40 origin-containing DNA during infection with a recombinant Autographa californica multiple nuclear polyhedrosis virus expressing large T antigen.
... DNA replication in simian virus 40 (SV40) has been extensively ...The virus possesses just one origin and encodes only a single protein involved in the replication of its DNA, ... See full document
6
Simian Virus 40 Large T Antigen and Two Independent T-Antigen Segments Sensitize Cells to Apoptosis following Genotoxic Damage
... The simian virus 40 (SV40) large tumor (T) antigen is sufficient to transform cells in cultures and induce tumors in experimental ...primary cells in cultures ... See full document
13
Cell Cycle Progression in Monkey Cells Expressing Simian Virus 40 Small t Antigen from Adenovirus Vectors
... of large-T, we used defective adenoviruses that expressed WT or mutant small-t under the control of the cytomegalovirus promoter (42, ...CV1 cells at 48 h ...Adenoviruses expressing ... See full document
8
Primary rat cells expressing a hybrid polyomavirus-simian virus 40 large T antigen have altered growth properties.
... Although the ability of SV40 large T antigen to cooperate with activated Ha-ras to form foci is well documented 13, 19, 20, 36, 37, 49, the observation of two types of morphologies for c[r] ... See full document
10
A deletion in the simian virus 40 large T antigen impairs lytic replication in monkey cells in vivo but enhances DNA replication in vitro: new complementation function of T antigen.
... 2195-2207 0022-538X/92/042195-13$02.00/0 Copyright © 1992, American Society for Microbiology A Deletion in the Simian Virus 40 Large T Antigen Impairs Lytic Replication in Monkey Cells I[r] ... See full document
13
Constitutive expression of simian virus 40 large T antigen in monkey cells activates their capacity to support polyomavirus replication.
... Similar results were observed with three different DNAs containing wild-type polyomavirus origins, and we have observed replication of polyomavirus DNAs in two other types of monkey cell[r] ... See full document
5
Archetype JC Virus Efficiently Replicates in COS-7 Cells, Simian Cells Constitutively Expressing Simian Virus 40 T Antigen
... of replication of JCV in POJ cells. The replication of archetype JCV in POJ cells was detected by the continuous presence of faint hybridization signals during the incubation of transfected ... See full document
8
Replication and transformation functions of in vitro-generated simian virus 40 large T antigen mutants.
... Mutant of simian virus 40 large T-antigen that is defective for viral DNA synthesis, but competent for transformation of cultured rat cells.. Simian virus 40 deoxyribonucleic acid synthe[r] ... See full document
10
Formation of Covalently Modified Folding Intermediates of Simian Virus 40 Vp1 in Large T Antigen-Expressing Cells
... the large T (LT) and small t (ST) antigens, are also J domain proteins (24–28) and interact with Hsc70 ...DNA replication, transcriptional regula- tion, transformation, and virion maturation ... See full document
12
Expression and complex formation of simian virus 40 large T antigen and mouse p53 in insect cells.
... Analysis of SVT-Ag and p53 extracted from SF21 cells coinfected with both vEV55SVT and vEV55p53 reveals no major differences in the degree of phosphorylation of either of these proteins [r] ... See full document
11
Association of a cellular heat shock protein with simian virus 40 large T antigen in transformed cells.
... To establish whether p73 is related to the hsp70 family of proteins, we attempted to immunoprecipitate the p73/T-ag complexes from cellular extracts of ST803 cells by using a monoclonal [r] ... See full document
13
Association of a murine 53,000-dalton phosphoprotein with simian virus 40 large-T antigen in transformed cells.
... 213-224 0022-538X/80/04-0213/12$02.00/0 Association of a Murine 53,000-Dalton Phosphoprotein with Simian Virus 40 Large-T Antigen in Transformed Cells FRANK McCORMICK* AND ED HARLOW and [r] ... See full document
12
Detection and characterization of multiple forms of simian virus 40 large T antigen.
... Simian virus 40 SV40 DNA codes for two early proteins, the large and small tumor T antigens 22, 30, 33, which can be immunoprecipitated from extracts of cells productively infected or tr[r] ... See full document
11
Rapid and sensitive quantitative immunoassay for the large simian virus 40 T antigen.
... To confirm that this approach applies Replicates containing 2.5, 7.5, 12.5, and 17.5 Al of a to the quantitation of highly purified large T partiallypurified SV80 large-T-antigen fractio[r] ... See full document
9
Analysis of simian virus 40 small t antigen-induced progression of rat F111 cells minimally transformed by large T antigen.
... To establish such a system, we infected rat Flll cells with the retroviral large T expression vector pZIPTEX 7, which directs the expression of limiting amounts of large T and thus Cellu[r] ... See full document
9
Simian virus 40 T-antigen-related cell surface antigen: serological demonstration on simian virus 40-transformed monolayer cells in situ.
... In a different approach to demonstrate the location of SV40 T-Ag-related molecules on the outer surface of the plasma membrane, living SV3T3 cells were treated as an intact monolayer wit[r] ... See full document
14
Two separable functional domains of simian virus 40 large T antigen: carboxyl-terminal region of simian virus 40 large T antigen is required for efficient capsid protein synthesis.
... COLE',2* Department Genetics, Yale University School of Medicine, New Haven, Connecticut 065101; and Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 037562* [r] ... See full document
10
Immunological Cross-Reactivity Between Simian Virus 40 Large T Antigen and D2 Hybrid T Antigen
... Proteins were immunoprecipitated from these extracts by using the antiserum produced in rabbits against purified D2 hybrid T antigen rabbit anti-D2, and these proteins were compared with[r] ... See full document
5
Improved localization of phosphorylation sites in simian virus 40 large T antigen.
... Amino acid sequence analysis of fragments generated by partial proteolysis from large simian virus 40 tumor antigen.. Binding of dephosphorylated A protein to SV40 DNA.[r] ... See full document
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