[PDF] Top 20 Roles of the Phosphorylation of Herpes Simplex Virus 1 UL51 at a Specific Site in Viral Replication and Pathogenicity

... identified phosphorylation sites in UL51, serine 184 (Ser-184), but not the other identified phosphorylation sites, significantly re- duced viral replication and cell-cell spread in HaCaT ... See full document

... expressed herpes simplex virus 1 (HSV-1) regulatory protein ICP22 coupled with mass spectrometry-based proteomics technology and subsequent analyses showed that ICP22 interacted with ... See full document

... of viral replication and explant-in- duced reactivation observed with Phos 2 only became apparent when tested in the mouse ocular model, highlighting the im- portance of characterizing our mutants in ...one ... See full document

... 2009 Herpes simplex virus 1 (HSV-1) protein ICP27 is a multifunctional regulatory protein that is posttransla- tionally modified by phosphorylation during viral ...(PKA) ... See full document

... impaired viral replication in eyes, ears, or vaginas of mice following ocular, ear, or vaginal inoculation, respectively (49, ...this phosphorylation was needed for infection of the CNS of mice but ... See full document

... DISCUSSION Virus replication can begin only after the viral genome has been delivered to a site capable of supporting the production of viral gene products and replication of the ... See full document

... HSV-1 replication and cell-cell spread in SK-N-SH cells and reduced HSV-1 neurovirulence in mice following intracranial ...Thr-111 phosphorylation is required for both efficient HSV-1 ... See full document

... wild-type viral replication in the eyes at 4 days postinfec- tion and in the brains at 3 and 4 days ...reduced viral replication in the trigeminal ganglia caused by the preclusion of Us8A ... See full document

... the viral regulatory proteins just mentioned with the exception of US11, casein kinase II, cdk-1, and protein kinase A have been implicated in their subcellular localization, and putative ... See full document

... HSV-1 replication by increas- ing the fidelity of viral DNA ...immunodeficiency virus (FIV) mutant lacking vdUTPase that was integrated into the DNA of T lymphocytes was signifi- cantly lower ... See full document

... the phosphorylation or potential phosphorylation of different res- idues within region ...phorylation site mutations (S224A, T226A, S224A/T226A, T231A, and T232A) were constructed, and the ... See full document

... are specific. Growth defects of the UL51 deletion mutant. While UL51 is not essential for HSV-1 replication, previous work showed that a deletion mutant removing both UL50 and ... See full document

... to virus-mediated gene therapy, where viral replication and associated cytotoxicity are limited to a specific cell type by the regulated expression of an essential immediate-early viral ... See full document

... whereby virus infections induce immune reactions that subse- quently involve self components (34, ...of virus replication in initiating ...although virus and demon- strable antigens are absent ... See full document

... in virus-infected ...␥ 1 34.5 on STING phosphorylation, a hallmark of activation ...STING phosphorylation at serine ...(aa 1 to 146) was deleted (29), also caused STING ...repair ... See full document

... in viral replication in cell cultures and their important role in neurovirulence in mice was of interest, but similar observations have been observed with other HSV-1 func- ...impaired viral ... See full document

... produce virus-related parti- cles under conditions in which viral DNA synthesis is blocked either genetically or ...of viral DNA synthesis and hence of the production of infectious virus, we ... See full document

... lytic replication, though recent reports have differed on this point [54, ...the viral DNA must recombine by means other than canonical ATM- dependent homologous recombination, perhaps through proteins that ... See full document

... the virus enters mucosal tissue and replicates. 2,3 Once the virus has completed initial infection, it resides in sensory nerve ganglia and enters ...the viral genome is characterized by repressive ... See full document

... by phosphorylation of the tyrosine residue that is conserved in herpesviruses and that this regulation played an important role in viral replication and ...for viral pathogenesis in vivo, ... See full document