[PDF] Top 20 Routine use of PCR restriction fragment length polymorphism analysis for identification of mycobacteria growing in liquid media
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Routine use of PCR restriction fragment length polymorphism analysis for identification of mycobacteria growing in liquid media
... of PCR-RFLP’s ability to dif- ferentiate the ...other mycobacteria growing in liquid ...to PCR-RFLP and could probably be performed by many of the PCR-based procedures that have ... See full document
7
Evaluation of PCR Restriction Profile Analysis and IS2404 Restriction Fragment Length Polymorphism and Amplified Fragment Length Polymorphism Fingerprinting for Identification and Typing of Mycobacterium ulcerans andM marinum
... comparative analysis including PCR restriction profile analysis (PRPA), IS2404 restriction fragment length polymorphism (RFLP), and amplified fragment ... See full document
7
Species Identification of Mycobacteria by PCR Restriction Fragment Length Polymorphism of therpoB Gene
... Currently, identification of clinical isolates of mycobacteria to the species level is primarily based on cultural characteristics and biochemical ...performance liquid chromatography, ... See full document
6
Identification of Oral Peptostreptococcus Isolates by PCR Restriction Fragment Length Polymorphism Analysis of 16S rRNA Genes
... The use of gas-liquid chromatography (GLC) for detecting VFA by-products of bacterial metabolism has been touted as an additional method for identifying Peptostreptococcus species (7, 8, ...specific ... See full document
5
Molecular Typing of Nocardia Species
... Conventional identification of Nocardia species in routine medical laboratories which is based on phenotypic (cellular morphology, colonial characteristics), biochemical and enzymatic profiles, and ... See full document
8
Identification of 54 Mycobacterial Species by PCR Restriction Fragment Length Polymorphism Analysis of the hsp65Gene
... a liquid medium (MB/BacT system; Organon Teknika ...pigmentation. Identification at the species level was done by classical biochemical identification tests and AccuProbe tests (Gen-Probe Incor- ... See full document
8
PCR amplification and restriction endonuclease analysis of a 65 kilodalton heat shock protein gene sequence for taxonomic separation of rapidly growing mycobacteria
... rapidly growing mycobacteria (RGM) belonging to 10 taxonomic groups were studied for restriction fragment length polymorphism patterns from a PCR-amplified 439-bp segment ... See full document
5
Rapid identification of Campylobacter species by restriction fragment length polymorphism analysis of a PCR amplified fragment of the gene coding for 16S rRNA
... Restriction fragment length polymorphism analysis of a PCR-amplified DNA fragment of the gene coding for 16S rRNA was performed on 148 previously characterized strains of ... See full document
6
PCR Restriction Fragment Length Polymorphism Analysis for Identification of Bacteroides spp and Characterization of Nitroimidazole Resistance Genes
... combined PCR-restriction fragment length polymorphism (RFLP) technique to characterize the 16S rRNA gene for identification purposes and the nitroimidazole resistance (nim) gene ... See full document
5
Rapid identification of mycobacteria to species level by PCR restriction fragment length polymorphism analysis of the hsp65 gene and proposition of an algorithm to differentiate 34 mycobacterial species
... biochemical identification tests and the Accuprobe tests and DT1-DT6 PCR (7) for the ...for PCR. PRA based on the amplification of a 439-bp fragment of the hsp65 gene was performed with ... See full document
5
Detection of Cryptosporidium and Identification to the Species Level by Nested PCR and Restriction Fragment Length Polymorphism
... nested PCR and restriction fragment length polymorphism (RFLP) that offers simple identification of Cryptosporidium hominis, Cryptosporidium parvum, and most other human ... See full document
7
Identification of Infectious Agents in Onychomycoses by PCR Terminal Restriction Fragment Length Polymorphism
... TRFLP analysis was used to identify infectious fungi based on differences in their 28S rDNA ...and identification (7, 27, 28). The polymorphism of the in- ternal transcribed spacers (ITS) of ... See full document
9
Rapid Identification and Typing ofStaphylococcus aureus by PCR Restriction Fragment Length Polymorphism Analysis of the aroA Gene
... Staphylococcus aureus is the causative agent of many oppor- tunistic infections in humans and animals (8). As a human pathogen, S. aureus causes superficial, deep-skin, and soft- tissue infections, endocarditis, and ... See full document
5
Molecular Identification of Capnocytophaga spp via 16S rRNA PCR Restriction Fragment Length Polymorphism Analysis
... The number of clinical isolates (187) identified by using this strategy was deemed to constitute a representative sample of the collection of Capnocytophaga isolates (n ⫽ 848) obtained as a result of this investigation. ... See full document
8
Identification of cagA Tyrosine Phosphorylation DNA Motifs in Helicobacter pylori Isolates from Peptic Ulcer Patients by Novel PCR Restriction Fragment Length Polymorphism and Real Time Fluorescence PCR Assays
... sequence analysis of amplicons. After block cycler PCR amplifi- cation, cagA amplicons from 37 strains were purified and then sequenced in- house by using the CEQ Dye Terminator Cycle Sequencing Quick Start ... See full document
7
Single Nucleotide Polymorphisms in the IS900 Sequence of Mycobacterium avium subsp paratuberculosis Are Strain Type Specific
... as restriction fragment length polymorphism analysis with hybridization to IS900 (19), pulsed-field gel electrophoresis (PFGE) (11, 29), PCR-restriction enzyme ... See full document
5
Development and Application of a New Scheme for Typing Campylobacter jejuni and Campylobacter coli by PCR Based Restriction Fragment Length Polymorphism Analysis
... with restriction fragment length polymorphism analysis of a ...9.6-kb PCR-amplified portion of the lipopolysaccharide gene ... See full document
7
Original Article Interleukin-16 gene polymorphism is associated with acute coronary syndrome in the Chinese Han population
... ACS refers to a group of clinical heart disease caused by myocardial ischemia [21]. Resea- rches have showed that genetic polymorphism of IL-16 is associated with multiple human dis- eases [22-24]. In the current ... See full document
7
Evaluation of Ficolin-3 (FCN3) 1637delC (rs28357092) Frameshift Mutation in Iranian Type 2 Diabetic Subjects
... immunity. Polymorphism in ficolin-3 gene (FCN3) can lead to complement deficiency and increase the risk of some disorders such as ...nucleotide polymorphism in this gene in healthy and diabetic subjects of ... See full document
7
Diversity of Staphylococcus Species Strains Based on Partial kat (Catalase) Gene Sequences and Design of a PCR Restriction Fragment Length Polymorphism Assay for Identification and Differentiation of Coagulase Positive Species (S aureus, S delphini, S hyicus, S intermedius, S pseudintermedius, and S schleiferi subsp coagulans)
... rate identification of staphylococcal species by microbiological laboratories, previous studies demonstrated the unreliability of phenotypic methods, including the Vitek 2 system (bio- Me ´rieux, Marcy l’Etoile, ... See full document
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