[PDF] Top 20 Simian virus 40 tumor antigen: isolation of the origin-specific DNA-binding domain.

... To obtain direct evidence that this region of the T antigen molecule is not only conformationally important but is, in fact, sufficient to act as the binding site with specificity for th[r] ... See full document

... SV40 origin of ...the origin-binding region including residues 121 to 135 is involved in A/T untwisting but not in IP ...two DNA sequences, as well as the reactions that take place at the two ... See full document

... Mutations in the phosphorylation sites of simian virus 40 SV40 T antigen alter its origin DNA-binding specificity for sites I and II and affect SV40 DNA replication activity.. Initiation[r] ... See full document

... Non-sequence- specific binding events may explain, at least in part, assembly on the 48-bp site IIm ⫹ EP and 47-bp site IIm ⫹ AT oligo- ...core origin subdomain is flanked on at least one side by ... See full document

... However, a description of the precise protein-DNA interactions of T antigen with these two different sites at a level comparable to that of procaryotic DNA-binding proteins 33 requires m[r] ... See full document

... Similar treatment of the truncated 259.3 protein produced in HeLa cells resulted in only a slight if any increase in binding to both wt origin- and site Il-containing fragments.. Two int[r] ... See full document

... Eukaryotic DNA replication is a complex process that is initiated by the coordinated action of several factors, including the origin recognition complex, cdt1, cdc6, and the minichro- mosome maintenance ... See full document

... forked DNA sub- strate is presented in ...forked DNA oligonucleotide. (A) The sequence of the forked DNA target used in crystallization indicating duplex DNA and ssDNA ...the DNA ... See full document

... for DNA replication; the references to the individual mutations are ...in binding to double-stranded DNA substrates (i.e., the core origin [0], site I [I], site II [II], an origin ... See full document

... Simian virus 40 large tumor antigen requires three core replication origin domains for DNA unwinding and replication in vitro.. Simian virus 40 SV40 DNA replication: SV40 large T antigen[r] ... See full document

... 4858 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest We have previously identified three regions called elements in the DNA-binding domain of simian virus 40 large tumo[r] ... See full document

... We can speculate that, although the newly synthesized 5 to 7S T antigen form is the active viral origin-binding form, the subsequently formed more rapidly sedimenting T antigen oligomers[r] ... See full document

... of DNA is displaced from the channel is not clear, but it probably involves a conformation change of the T-anti- gen ...helicase domain may provide an ...helicase domain forms a fila- ment instead of ... See full document

... In addition to identifying three discrete strong binding regions adjacent to the viral replication origin, several experiments indicated that Py T antigen binds weakly but specifically t[r] ... See full document

... for DNA, as we have observed for the related DBD of the papillomavirus E1 helicase ...T- antigen OBD would indeed increase its affinity for DNA, we performed binding experiments with the ... See full document

... eukaryotic DNA replication, at the molecular level, have been provided by model systems, in particular, the small DNA tumor viruses including simian virus 40 (SV40) and ... See full document

... Specific binding to the unmethylated 1,229-base-pair fragment was not observed, even at higher T-antigen concentrations, nor was there binding to the smaller 222-base-pair plasmid fragme[r] ... See full document

... These activities include origin-specific and nonspecific DNA binding, melting of the imperfect palindromic sequence, untwisting of the AT-rich region, unwinding of origincontaining DNA, [r] ... See full document

... The variants used to map the DNA-binding domain include: i fragments of large T produced by in vitro translation of unspliced SV40 cRNA 42; ii various truncated forms of large T present [r] ... See full document

... free DNA or protein-DNA complexes, were excised and eluted overnight at 378C in 550 ml of elution buffer ...centrifugation, DNA-containing solutions were subjected to extractions with ... See full document