[PDF] Top 20 Herpes Simplex Virus Type 1 Helicase-Primase: DNA Binding and Consequent Protein Oligomerization and Primase Activation
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Herpes Simplex Virus Type 1 Helicase-Primase: DNA Binding and Consequent Protein Oligomerization and Primase Activation
... to DNA in the presence of AMP-PNP. (iii) Primase activity assays reveal co- operative dependence on protein concentration while ATPase and helicase activities do ...as protein ... See full document
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Mutations in the Putative Zinc-Binding Motif of UL52 Demonstrate a Complex Interdependence between the UL5 and UL52 Subunits of the Human Herpes Simplex Virus Type 1 Helicase/Primase Complex
... a 1:1:1 ratio of the three proteins in the helicase-primase com- plex ...the binding properties of the dye itself, which is somewhat dependent on amino acid ...UL52 ... See full document
9
DNA-Dependent Oligomerization of Herpes Simplex Virus Type 1 Regulatory Protein ICP4
... multiple protein-DNA complexes with long DNA ...ICP4 protein was incubated with DNA probes of various sizes, all containing a single ICP4 binding site, and then run on native ... See full document
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Helicase Motif Ia Is Involved in Single-Strand DNA-Binding and Helicase Activities of the Herpes Simplex Virus Type 1 Origin-Binding Protein, UL9
... conserved helicase motif Ia were generated by two-step primer PCR ...and DNA sequencing. pFastBac BamHI/EcoRI fragments encoding wild-type and mutant UL9 genes were subcloned into the pCDNA1 vector ... See full document
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Baculovirus Replication Factor LEF-1 Is a DNA Primase
... the primase active site (3). The lef-1 gene of AcMNPV encodes a protein of 266 amino acids with a calculated molecular mass of 30,780 ...In herpes simplex virus type ... See full document
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Properties of the novel herpes simplex virus type 1 origin binding protein, OBPC.
... HSV-1 DNA replication, and of the potential role of OBPC in this process, is a consideration of the structure of replicative inter- ...HSV-1 DNA is likely replicated by a theta mechanism early ... See full document
7
Inhibition of PKR Activation by the Proline-Rich RNA Binding Domain of the Herpes Simplex Virus Type 1 Us11 Protein
... dsRNA binding motifs that in part mediate the dimerization of the enzyme on the dsRNA ...of activation and imbuing the enzyme with the ability to phosphorylate other substrates in trans, notably the ... See full document
7
Inhibition of Herpes Simplex Virus Type 1 Replication by Adeno-Associated Virus Rep Proteins Depends on Their Combined DNA-Binding and ATPase/Helicase Activities
... as DNA replication, regulation of gene expression, ge- nome packaging, and site-specific integration (reviewed in ref- erence ...AAV DNA metabolism include site-specific DNA-binding and ... See full document
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Recruitment of Polymerase to Herpes Simplex Virus Type 1 Replication Foci in Cells Expressing Mutant Primase (UL52) Proteins
... ICP8, helicase-primase, UL9, HSV Pol, and UL42 as well as the cellular protein PML, appears to depend on the activity of the ...active primase are experiments performed by Dracheva et ... See full document
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The conserved helicase motifs of the herpes simplex virus type 1 origin-binding protein UL9 are important for function.
... Binding of the herpes simplex virus type 1 UL9 gene product to an origin of viral DNA replication.. Nucleic Acids Res.[r] ... See full document
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Phosphorylation of the Herpes Simplex Virus Type 1 Origin Binding Protein
... The herpes simplex virus type 1 (HSV-1) origin binding protein (OBP), the product of the UL9 gene, is one of seven HSV-encoded proteins required for viral ... See full document
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The catalytic subunit of the DNA polymerase of herpes simplex virus type 1 interacts specifically with the C terminus of the UL8 component of the viral helicase-primase complex.
... peptide 5 more active than peptide 4. It is tempting to specu- late that at least some of these six residues may be directly involved in the interaction of UL8 with POL, but further stud- ies are necessary to determine ... See full document
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An Intertypic Herpes Simplex Virus Helicase-Primase Complex Associated with a Defect in Neurovirulence Has Reduced Primase Activity
... quence. Virus replication, however, requires a large number of specific interactions between viral polypeptides, and therefore it is perhaps not surprising that many intertypic recombinants containing a mixture of ... See full document
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Identification and characterization of a DNA primase activity present in herpes simplex virus type 1-infected HeLa cells.
... Percentage of DNA primase activity remaining in solution after treatment with anti-calf peptide IgGs and after binding of the antibody-antigen complex to protein A-Sepharose" Enzyme % Ac[r] ... See full document
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Herpes Simplex Virus Type 1 DNA-Packaging Protein UL17 Is Required for Efficient Binding of UL25 to Capsids
... UL17-stop virus contains a 16-bp insertion at position 32718 in the HSV-1 strain 17 genome, with stop codons in all three reading frames, truncating the UL17 open reading frame after codon 260 ...with ... See full document
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Thermolabile in vivo DNA-binding activity associated with a protein encoded by mutants of herpes simplex virus type 1.
... 3 Thermolabile In Vivo DNA-Binding Activity Associated with a Protein Encoded by Mutants of Herpes Simplex Virus Type 1 Received 30 December 1982/Accepted 17 March 1983 The major DNA-bin[r] ... See full document
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Mutational Analysis of the Herpes Simplex Virus Type 1 DNA Packaging Protein UL33
... (AcUL33 virus) or mutated (Acin14 to Acin116) forms of UL33 and with AcUL28Myc, expressing a c-Myc-tagged form of ...lanes 1 to 3) demonstrating that precipitation of UL28-Myc by R148 is specifically ... See full document
8
Kinetics of expression of the gene encoding the 65-kilodalton DNA-binding protein of herpes simplex virus type 1.
... To characterize more thoroughly the kinetics with which the 65KDBP gene is expressed, we have examined not only the accumulation of 6SKDBP mRNA and protein, as compared to other well-cha[r] ... See full document
11
DNA binding and gene regulation by the herpes simplex virus type 1 protein ICP4 and involvement of the TATA element.
... The regions of the herpes simplex virus type 1 immediate early protein Vmw 175 required for site specific DNA binding closely correspond to those involved in transcriptional regulation..[r] ... See full document
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Interaction with nucleic acids and stimulation of the viral DNA polymerase by the herpes simplex virus type 1 major DNA-binding protein.
... 727 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The interaction of the herpes simplex virus type 1 HSV-1 major DNA-binding protein, infected-cell polypeptide 8 ICP8[r] ... See full document
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