[PDF] Top 20 Simultaneous Quantification and Genotyping of Hepatitis B Virus for Genotypes A to G by Real Time PCR and Two Step Melting Curve Analysis
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Simultaneous Quantification and Genotyping of Hepatitis B Virus for Genotypes A to G by Real Time PCR and Two Step Melting Curve Analysis
... Hepatitis B virus (HBV) infection is an important public health problem chronically infecting more than 350 million people ...into genotypes A to H, with an intergenotypic diversity of at ... See full document
7
Simultaneous Genotyping and Quantification of Hepatitis B Virus for Genotypes B and C by Real Time PCR Assay
... for quantification was compared with that of a commercial assay with good statistical ...HBV genotyping obtained by the GQ-PCR assay and that obtained by direct sequencing (Table ...mixed ... See full document
8
Novel Method for Genotyping Hepatitis B Virus on the Basis of TaqMan Real Time PCR
... of real-time PCR, by which each genotype can be specifically amplified and/or ...in hepatitis B infections, especially among patients on treatment, has been reported by several ...of ... See full document
7
Simultaneous detection of porcine pseudorabies virus, porcine parvovirus and porcine circovirus type 2 by multiplex real-time PCR and amplicon melting curve analysis using SYBR Green I
... employ PCR. However, it would be more efficient if more than one virus could be detected in one ...Multiplex real-time PCR can be used for simulta- neous amplification of two or ... See full document
9
Real time RT PCR high resolution melting curve analysis and multiplex RT PCR to detect and differentiate grapevine leafroll associated virus 3 variant groups I, II, III and VI
... HRM analysis and assign each sample to a specific variant ...A two- step RT-PCR multiplex protocol was used and approxi- mately 1000-1500 ng of total RNA was denatured at 65°C for 5 minutes ... See full document
12
Detection of a Knockdown Resistance Mutation Associated with Permethrin Resistance in the Body Louse Pediculus humanus corporis by Use of Melting Curve Analysis Genotyping
... single-step melting curve analysis genotyping ...The melting curve analysis genotyping technique is also used in other fields, such as molecu- lar ... See full document
5
Performance of the Cobas AmpliPrep/Cobas TaqMan Real Time PCR Assay for Hepatitis B Virus DNA Quantification
... Group B comprised 52 patients with chronic HBV infection, all of whom had detectable HBsAg, anti-HBc antibodies, and HBV ...HBV genotyping assay (Innogenetics, Gent, Belgium), a reverse hybridization assay ... See full document
8
Detection and Genotyping of Oocysts of Cryptosporidium parvum by Real Time PCR and Melting Curve Analysis
... Several real-time PCR methods for detection and geno- typing of ...ventional PCR, the sensitivity of the real-time method was strongly dependent on the amplification primers, ... See full document
8
TaqMan Real Time Reverse Transcription PCR Assay for Universal Detection and Quantification of Avian Hepatitis E Virus from Clinical Samples in the Presence of a Heterologous Internal Control RNA
... published real-time PCRs for human and swine HEV (1, 8, 10, 19, 20, 26, 33) have a lower detection limit, but they use DNA as a standard, except for one assay that also used in vitro-transcribed RNA ... See full document
8
Development of a melting curve based multiplex real time PCR assay for simultaneous detection of Streptococcus agalactiae and genes encoding resistance to macrolides and lincosamides
... S1. Melting curve analysis showing the melting temperature peaks (Tm) of RNaseP (A) and IGS1 (B) ...potential PCR interfering substances, respectively. The multiplex ... See full document
11
Simultaneous Detection, Genotyping, and Quantification of Human Papillomaviruses by Multicolor Real Time PCR and Melting Curve Analysis
... 96-well real-time thermocycler, and 120 samples could be tested per run if the sys- tem is changed to a 384-well ...the time is spent in the amplification stage, the combined use of extra standard ... See full document
7
Review: Molecular Diagnosis of Hepatitis C Viruses; Technologies and Its Clinical Applications
... have two to three fold higher response rate than the patients infected with genotype 1 ...HCV genotyping assay for treatment management and ultimate patient care is ...sequencing analysis of specific ... See full document
33
Real Time PCR Assay for Detection and Quantification of Hepatitis B Virus Genotypes A to G
... HBV real-time PCR assay to determine clinical ...testing. PCR primers and probe ...“human hepatitis B virus” and “complete ...including genotypes A to H from ... See full document
9
Abbott RealTime Hepatitis C Virus (HCV) and Roche Cobas AmpliPrep/Cobas TaqMan HCV Assays for Prediction of Sustained Virological Response to Pegylated Interferon and Ribavirin in Chronic Hepatitis C Patients
... tion-mediated amplification assay (the lower limit of detection is 5 to 10 IU/ml) at the end of treatment in the patients who reached SVR (16). One possibility is that in some patients with SVR, HCV RNA became ... See full document
5
Detection and Differentiation of In Vitro Spiked Bacteria by Real Time PCR and Melting Curve Analysis
... These two bacteria have very similar melting point characteristics, so they could only be identified as “Staphylococcus ...the melting tempera- tures of the hybridization probes only, whereas other ... See full document
6
Rapid and Specific Genotyping System for Hepatitis B Virus Corresponding to Six Major Genotypes by PCR Using Type Specific Primers
... for PCR, by which HBV isolates can be classified into genotypes A through F is ...of PCR typing, phylogenetic analysis in the pre-S1 through S genes of HBV was also performed, and we confirmed ... See full document
5
Validation and implementation of hepatitis b viral load detection with quantitative real time PCR
... Hepatitis B viral (HBV) load monitoring is mainly important for the patients suffering from liver related ...and time PCR for Hepatitis B viral load ...digital PCR for the ... See full document
5
An Inexpensive and Accurate Reverse Transcription PCR–Melting Temperature Analysis Assay for Real Time Influenza Virus B Lineage Discrimination
... influenza B-positive specimens, collected at Brazilian State Public Health Laboratories during 2004, 2008, and 2010 to 2017 and characterized in our laboratory (National Influenza Centre, FIOCRUZ, Rio de Janeiro, ... See full document
8
Detection, quantification and genotyping of Herpes Simplex Virus in cervicovaginal secretions by real time PCR: a cross sectional survey
... HSV PCR products were diluted one in ten with DNase free RNase free water and probe typed using Taqman MGB probes (Applied Biosystems, Inc, Warrington, UK) directed against the HSV DNA polymer- ase I ...tube ... See full document
10
Rapid Differentiation of Mycobacterium tuberculosis and M bovis by High Resolution Melt Curve Analysis
... a real-time PCR HRM assay on DNA extracted directly from positive Ziehl-Neelsen-stained sputum smear ...by PCR prior to the real-time PCR HRM assay was found to be crucial ... See full document
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