[PDF] Top 20 Stoichiometry of Antibody Neutralization of Human Immunodeficiency Virus Type 1

... of antibody molecules required to neutralize a particular HIV-1 virion must vary considerably depending upon arbitrary factors, such as the age and handling of the virus ...a virus such as ... See full document

... stomatitis virus G glycoprotein, indicates that at least 2 logs of this defectiveness can be attributed to the HIV-1 Env glycoproteins ...using antibody neutralization ...neutralizing ... See full document

... against neutralization as well as in directly contributing to unique neutralizing ...simian immunodeficiency virus (SIV) infection has come from the study of mutants that eliminate glycans either ... See full document

... The parental MT-CSF infected only PM-1 cells and was markedly resistant to neutralization by sera from HIV-1-infected individuals, rabbit antiserum to recombinant MT-CSF gpl20, and anti-[r] ... See full document

... sorbing antibody-coated virus to CD4 ⫹ lymphocytes at 4°C, thereby blocking fusion and ...cells. Antibody bound to ad- sorbed virus by its Fab could interact through its Fc segment with NK ... See full document

... Human immunodeficiency virus type 1 (HIV-1) encodes a gp160 envelope precursor glycoprotein. HIV-1 envelope gly- coprotein spikes mature by trimerization, glycosylation, ... See full document

... mutant virus. The ef- fects of these mutations on the neutralization sensitivity of the LLP-2 mutant virus were assessed in primary human cells by highly sensitive flow cytometry-based ... See full document

... vitro neutralization has been correlated with protection against primary viruses in animal ...a neutralization escape mutant to one of the antibodies (b12) selected in vitro remains sensitive to ... See full document

... (19). Virus was generated by env transcomplementation by cotransfection of equal amounts of pSV-A308 and pSV-A308T321, pSV-A308T321, and pSV- A308K373 and by transfection of pSV-A308K373 ...lope-complemented ... See full document

... Antibody neutralization of viruses in vitro is an important phenomenon, since there is generally a good correlation be- tween in vitro neutralization and in vivo antiviral efficacy (13, ...of ... See full document

... of virus entry and neu- tralization quantitatively (14, 17, 18, ...the virus to infect target cells—commonly referred to as the stoichiometry of (virus) entry—on which we focus in this pa- ... See full document

... and neutralization experi- ments to maximize the opportunities for detecting relevant ...gp120-adsorbed antibody prepara- ...as neutralization of the laboratory-adapted strain HxB2, but had limited ... See full document

... the antibody epitope ...to neutralization by that ...to neutralization by 4E10, even though they contained sequences in the 4E10 epitope NWFDIT identical to those of variants that displayed ... See full document

... 2F5 neutralization were also re- quired for SC44 serum ...purified antibody (SC44 puri) was concentrated to ⬃ 500 ␮ ...for neutralization of TND_669S (solid lines), absorption of ... See full document

... Human immunodeficiency virus type 1 (HIV-1) uses a variety of chemokine receptors as coreceptors for virus entry, and the ability of the virus to be neutralized by ... See full document

... 1875-1883 0022-538X/92/041875-09$02.00/0 Copyright C 1992, American Society for Microbiology Mutations in the Principal Neutralization Determinant of Human Immunodeficiency Virus Type 1 [r] ... See full document

... Measurement of viral envelope glycoprotein-specific antibody avidity. The avidity index values of serum antibodies to the native viral envelope were deter- mined by measuring the resistance of serum ... See full document

... pro- virus (28) contains a BglII-BglII deletion within the envelope gene and a substitution of the viral nef gene with a cDNA encoding the murine ...simian virus 40 ori was subsequently introduced into the ... See full document

... length of time since receipt of vaccine, we performed an analysis limited to children whose serostatus was assessed within 1 year of vaccination. The proportion of children with detectab[r] ... See full document

... of human immunodeficiency virus type 1 (HIV-1) which have only been carried in vitro in peripheral blood mononuclear cells (primary isolates) can be neutralized by antibodies, ... See full document