[PDF] Top 20 Strand displacement synthesis capability of Moloney murine leukemia virus reverse transcriptase.
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Strand displacement synthesis capability of Moloney murine leukemia virus reverse transcriptase.
... If the entire plus strand is synthesized from one initiation event, as is generally depicted, our calculated rates suggest that displacement synthesis by the minus strand through the LTR[r] ... See full document
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Mutations of the RNase H C Helix of the Moloney Murine Leukemia Virus Reverse Transcriptase Reveal Defects in Polypurine Tract Recognition
... the synthesis of long minus- strand ...mutant virus synthesized slightly less mi- nus-strand DNA than the I597A and G602A mutant ...detectable synthesis of long minus-strand DNA ... See full document
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Structure-Based Moloney Murine Leukemia Virus Reverse Transcriptase Mutants with Altered Intracellular Direct-Repeat Deletion Frequencies
... Like the wild type, all mutants displayed increased rates of deletion when tested on a longer repeat, but mutant-specific effects were generally more modest with the 284-base repeat than with the 117-base repeat. In ... See full document
8
RNase H domain of Moloney murine leukemia virus reverse transcriptase retains activity but requires the polymerase domain for specificity.
... Specificity of tRNA primer removal by M-MuLV RNase H domain. We previously showed that the RNase H activity of M-MuLV RT removes the minus-strand tRNA primer by pref- erentially cleaving one nucleotide away from ... See full document
9
Specific Cleavages by RNase H Facilitate Initiation of Plus-Strand RNA Synthesis by Moloney Murine Leukemia Virus
... First, reverse transcriptase is much more facile at nondisplace- ment synthesis than displacement ...nondisplacement synthesis is about 5- to 10-fold more efficient than ... See full document
11
Identification of Residues of the Moloney Murine Leukemia Virus Nucleocapsid Critical for Viral DNA Synthesis In Vivo
... retard virus infection? NC has been shown to have impor- tant functions as an RNA ...facilitates reverse transcription both by reducing paus- ing at sites of secondary structure and by promoting ... See full document
11
YADD Mutants of Human Immunodeficiency Virus Type 1 and Moloney Murine Leukemia Virus Reverse Transcriptase Are Resistant to Lamivudine Triphosphate (3TCTP) In Vitro
... the virus is chal- lenged with 3TC either in culture or in a ...DNA synthesis is com- pleted by the wild-type enzyme, but slowly and inefficiently ... See full document
8
Effects on DNA synthesis and translocation caused by mutations in the RNase H domain of Moloney murine leukemia virus reverse transcriptase.
... translocated and elongated (60). This translocation defect probably results from the inability of these mutant RNases H to degrade their genomic RNA present in RNA-DNA hybrid form. By leaving minus-strand ... See full document
13
Structural Determinants of Murine Leukemia Virus Reverse Transcriptase That Affect the Frequency of Template Switching
... DNA synthesis (25% of an over- all deletion frequency of ...DNA synthesis is increased by approximately twofold with HU treatment, then the overall rate of direct repeat deletion would be expected to ... See full document
8
Mutations in the RNase H Primer Grip Domain of Murine Leukemia Virus Reverse Transcriptase Decrease Efficiency and Accuracy of Plus-Strand DNA Transfer
... DNA synthesis by S557A and Y586F mutant ...DNA synthesis, this junction is part of the primer strand and is formed during the initiation process by the extension of the tRNA ...provirus. Virus ... See full document
9
Abortive reverse transcription by mutants of Moloney murine leukemia virus deficient in the reverse transcriptase-associated RNase H function.
... Analysis of in vitro reverse transcription reactions carried out by mutant virions showed that minus-strand strong-stop DNA was formed but did not efficiently translocate to the 3' end o[r] ... See full document
11
Intramolecular Recombinations of Moloney Murine Leukemia Virus Occur during Minus-Strand DNA Synthesis
... plus- strand DNA ...necrosis virus-based system the recombination between two direct re- peats occurred at the same frequency during minus- and plus- strand DNA ...During reverse ... See full document
10
Characterization of Intracellular Reverse Transcription Complexes of Moloney Murine Leukemia Virus
... out reverse transcription in vitro, we performed an endogenous reverse transcription assay on 10-fold dilutions of the equilib- rium density fractions containing the peak of the strong-stop ...endogenous ... See full document
7
Replication Defect of Moloney Murine Leukemia Virus with a Mutant Reverse Transcriptase That Can Incorporate Ribonucleotides and Deoxyribonucleotides
... F155V virus to make full-length viral DNA was the selective inhibition of the mutant RT-F155V by ...wild-type virus reaction mixture, even at 100 mM, had no ...DNA synthesis level and not through ... See full document
7
Characterization of intracellular precursor polyproteins of Moloney murine leukemia virus.
... Recent studies have shown that Pr85'w incorporated [3H]mannose, bound to a 1051 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Intracellular Moloney munine leukemia vi[r] ... See full document
6
Effects of 3′ Untranslated Region Mutations on Plus-Strand Priming during Moloney Murine Leukemia Virus Replication
... frequent reverse tran- scription-associated deletion of a repeated enhancer element in U3, use of each PPT is represented on the gel in ...by virus with two wild-type PPTs ... See full document
10
Moloney Murine Leukemia Virus Integrase Protein Augments Viral DNA Synthesis in Infected Cells
... the virus life cycle (virion production or proteolytic processing of Gag and Pol), while others appeared to affect only early events ...DNA synthesis (8, 9, 24, ...the virus life cycle, we used MLV ... See full document
8
Inhibition by RNA of RNase H Activity Associated with Reverse Transcriptase in Rauscher Murine Leukemia Virus Cores
... To establish whether the apparent absence of RNase H activity in the core fraction signified a true separation of RNase H and reverse transcriptase activities or was caused by a hitherto[r] ... See full document
8
Development of an In Vivo Assay To Identify Structural Determinants in Murine Leukemia Virus Reverse Transcriptase Important for Fidelity
... selected. Virus harvested from the pool of transfected PG13 cells was used to infect D17 ...days. Virus was harvested and used to infect fresh D17 ...helper virus (data not ... See full document
8
Murine leukemia virus mutant with a frameshift in the reverse transcriptase coding region: implications for pol gene structure.
... Addition of plasmid DNAs containing the 5' portion of the mutant genome and the 3' portion of the wild-type line 1 gave rise to infectious virus with reverse transcriptase activity.. In [r] ... See full document
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