[PDF] Top 20 Structure and Assembly of the Sindbis Virus E1 and E2 Transmembrane Proteins
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Structure and Assembly of the Sindbis Virus E1 and E2 Transmembrane Proteins
... Sindbis virus is composed of two nested T=4 icosahedral protein shells containing 240 copies each of three structural proteins: E1, E2, and Capsid in a 1:1:1 stoichiometric ...ratio. ... See full document
102
A Single Deletion in the Membrane-Proximal Region of the Sindbis Virus Glycoprotein E2 Endodomain Blocks Virus Assembly
... at E2 position 391 affects the interaction(s) of the E2 tail with the cell membranes of vertebrate and in- vertebrate hosts in a differential ...of E2 K391 at the membrane junction leaves A392 at the ... See full document
9
Adaptive Mutations in Sindbis Virus E2 and Ross River Virus E1 That Allow Efficient Budding of Chimeric Viruses
... SIN E2 and RR E1 that result in the production of much more virus much more ...disparate proteins in a chimeric heterodimer to allow more efficient ...glyco- proteins to one another ... See full document
8
Molecular Genetic Study of the Interaction of Sindbis Virus E2 with Ross River Virus E1 for Virus Budding
... only E2 has a significant cytoplasmic tail required for budding, and in the chimera SIN E2 should be free to interact with SIN ...glyco- proteins must have arisen during speciation that lead to a ... See full document
6
Charged Residues in the Transmembrane Domains of Hepatitis C Virus Glycoproteins Play a Major Role in the Processing, Subcellular Localization, and Assembly of These Envelope Proteins
... glycoproteins E1 and E2 is not sufficient for an efficient arrest of ...(dengue virus type 4) ...envelope proteins in the Flaviviridae ...of E2 contains 6 leucine residues which could ... See full document
11
Single Amino Acid Insertions at the Junction of the Sindbis Virus E2 Transmembrane Domain and Endodomain Disrupt Virus Envelopment and Alter Infectivity
... the assembly of the alpha virion is the envelopment of the preassembled core structure within a virus protein-modified cell ...the E2 glycoprotein and a hydrophobic cleft in the capsid ... See full document
16
Investigation of the Role of the E2 Endodomain in Sindbis Virus Assembly
... Sindbis virus (SV) is the prototype of the alphaviruses, a family of viruses vectored in nature by mosquitoes and transferred via blood meal to humans and other vertebrates including birds and mammals ... See full document
100
The Surface Conformation of Sindbis Virus Glycoproteins E1 and E2 at Neutral and Low pH, as Determined by Mass Spectrometry-Based Mapping
... the virus spike complex at low ...spike proteins change confor- mation when the virus is exposed to low ...When Sindbis virus is exposed to low pH, a conformational change takes place ... See full document
12
Probing the Early Temporal and Spatial Interaction of the Sindbis Virus Capsid and E2 Proteins with Reverse Genetics
... envelope proteins in the ...envelope proteins could cotraffic to the plasma membrane ...envelope proteins form a 2D lattice that is planar enough to envelop the NC, providing the free energy to bud a ... See full document
12
Interactions between the Transmembrane Segments of the Alphavirus E1 and E2 Proteins Play a Role in Virus Budding and Fusion
... membrane proteins E1 and ...mature E1-E2 heterodimers, which are oligomerized into trimeric complexes, so-called spikes that bind both to each other and, at the PM, also to nucleocapsid (NC) ... See full document
10
Role of Rubella Virus Glycoprotein Domains in Assembly of Virus-Like Particles
... the transmembrane and cytoplasmic domains of E1 ...the assembly of rubella virus-like ...rubella virus-like particles to be secreted from transfected cells. The E1 ... See full document
10
Functional interactions between papillomavirus E1 and E2 proteins.
... about E2 and about the interaction between E1 and ...chimeric E2 proteins that we have tested, without exception, are active for replication under some conditions, providing good support for ... See full document
11
Carbohydrate Structure of Sindbis Virus Glycoprotein E2 from Virus Grown in Hamster and Chicken Cells
... Thus, Sindbis glycoprotein E2 acquires a complex acidic oligosaccharide and a high-mannose oligosaccharide when grown in BHK and chicken cells, whereas the vesicular stomatitis virus gly[r] ... See full document
9
Attenuating mutations in glycoproteins E1 and E2 of Sindbis virus produce a highly attenuated strain when combined in vitro.
... A recombinant, TR2000, generated by replacement of the TotollOl El and 6K gene sequences with those from an SB AR339 cDNA clone, differed from the attenuated TotollOl in that virus produ[r] ... See full document
7
Cellular and humoral immune responses to rubella virus structural proteins E1, E2, and C
... Using the expressed recombinant proteins as antigens, we were able to demonstrate antigen-specific lymphocyte proliferative responses in control individuals and individuals with congenit[r] ... See full document
7
The gene order for rubella virus structural proteins is NH2-C-E2-E1-COOH.
... By employing the widely used method of synchronizing initiation of protein synthesis by treatment of infected cells with hypertonic salt 14 in conjunction with pulse-chase labeling, immu[r] ... See full document
5
Deletions of Sindbis Virus E2 Endodomain Affect Virus Assembly and Identify the Virus Membrane as a Determinant of Host Range.
... virus assembly. In Figure 4 (A) BHK cells are shown infected with SVHR. Virus particles are seen bound to the plasma membrane and appear in various states of envelopment (budding, ... See full document
42
Bovine papillomavirus type 1 DNA replication: the transcriptional activator E2 acts in vitro as a specificity factor.
... protein E1, together with extract from a particular cell source, allowed the synthesis of complete DNA circles through successive rounds of replication; this occurred in the absence of the viral transcriptional ... See full document
11
THE CARCINOGENIC POTENTIAL OF E6 & E7 GENES OF HIGH RISK HPV COMPARED WITH E6, E7 GENES OF LOW RISK HPV IN HUMAN CERVICAL CANCER: A REVIEW
... infection by the human papilloma virus 4, 5 . The link between genital HPV infections and cervical cancer was first demonstrated in the early 1980s by Harold Zur Hausen, a German virologist. In 1996, the World ... See full document
10
Cell Fusion Activity of Hepatitis C Virus Envelope Proteins
... C virus (HCV) envelope proteins (E1 and E2), we have established a sensitive cell fusion assay based on the activation of a reporter gene as described previously ...HCV E1 and E2 ... See full document
9
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