[PDF] Top 20 Structures of herpes simplex virus type 1 genes required for replication of virus DNA.

... CHALLBERG2* Medical Research Council Virology Unit, Institute of Virology, University of Glasgow, Church Street, Glasgow GIl 5JR, United Kingdom,' and Laboratory of Viral Diseases, Natio[r] ... See full document

... wt virus and treated with PAA ...not required for ICP8 localization (3, ...(Table 1). Although the per- centage of UL42 mutant virus-infected cells showing a punc- tate distribution for ICP8 ... See full document

... Deproteinized replication products were loaded onto a solution of 3 ml of CsCl (refractive index 5 ...labeled DNA in each fraction was measured by the incorporation of [ ... See full document

... the virus life cycle and pathogenesis ...ant virus using BAC recombineering technology. The recombinant virus with the NES mutation in VP19C did not impair virus replication, and viral ... See full document

... with herpes simplex virus type 1 results in a chronic inflammatory response in the stroma termed herpetic stromal keratitis ...CD4 1 T cells of the type 1 cytokine ... See full document

... erpes simplex virus 1 (HSV-1) virions have three morpholog- ically distinct structures: the nucleocapsid, an icosahedral capsid containing the 152-kbp double-stranded DNA viral ... See full document

... Identification of the Oscar elements. As an alternative means of identifying specific flanking sequences that can enhance the function of the minimum core origin contained in pOS 80 (80), oriS-flanking sequences were ... See full document

... AN1 virus infection (43) and demonstrate that UL98 can restore viral repli- cation to near wild-type levels in the absence of UL12 and ...UL98 virus dem- onstrated a slight delay in viral ... See full document

... the DNA-containing capsid (32). The HSV-1 genome, like the ␾ 29 genome, is inserted into a very confined ...viral DNA is arranged in concentric rings with an interlayer spacing of about 26 Å, ... See full document

... the virus was purified from Benzonase-treated crude cell lysates over an iodixanol density gradient (Optiprep; Greiner Bio- One), followed by heparin-agarose type I affinity chromatography ...the ... See full document

... DAI is not essential for IFN- ␤ transcription through cytoso- lic B-DNA sensing in HepG2 cells. We investigated whether DAI plays an essential role in the type I IFN pathway in HepG2 cells after treatment ... See full document

... mutant virus with respect to the nuclear egress of nucleocapsids is, to some extent, similar to that of wild-type HSV-1(F) infection of nucleolin-depleted cells, nucleolin might interact with UL12 to ... See full document

... viral DNA synthesis pro- ceeds, the staining pattern evolves to a globular appearance as replication compartments begin to fill the nucleus (15, 48, ...globular structures in most cells, indicating ... See full document

... wild-type virus (30, 37), the availability of viral proteins for virion formation is also ...viral genes are affected during infection with ICP22 ⫺ ...is required to achieve wild-type ... See full document

... Herpes simplex virus type 1 (HSV-1) DNA replication intermediates exist in a complex nonlinear structure that does not migrate into a pulsed-field ...processing ... See full document

... The UL25 protein is associated with purified capsids. The fact that the UL25 protein is important for retaining cleaved DNA in capsids suggests that this protein may interact with capsids in vivo. It has been ... See full document

... compartments required viral DNA ...HSV-1 replication following knockdown of these two proteins ...large replication compartments that costained for ICP4 and ICP8 (80% of ...smaller ... See full document

... 3528 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest During the course of experiments designed to isolate deletion mutants of herpes simplex virus type 1 in the encodin[r] ... See full document

... 277 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest During the course of infection, elements of the herpes simplex virus type 1 HSV-1 genome undergo inversion, a proces[r] ... See full document

... Herpes simplex virus type 1 DNA replication occurs in nuclear domains termed replication compartments, which are areas of viral single-stranded DNA-binding ... See full document