[PDF] Top 20 Studies on the origin-specific DNA-binding domain of simian virus 40 large T antigen.
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Studies on the origin-specific DNA-binding domain of simian virus 40 large T antigen.
... Similar treatment of the truncated 259.3 protein produced in HeLa cells resulted in only a slight if any increase in binding to both wt origin- and site Il-containing fragments.. Two int[r] ... See full document
5
Quantitative Analysis of the Binding of Simian Virus 40 Large T Antigen to DNA
... to DNA showing that 70% of the protein-DNA contacts occur through these three nucleotides (4, ...vivo studies which showed that mutation of this residue from a G to an A reduced viral DNA ... See full document
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Crystal Structure of the Simian Virus 40 Large T-Antigen Origin-Binding Domain
... eukaryotic DNA replication, at the molecular level, have been provided by model systems, in particular, the small DNA tumor viruses including simian virus 40 (SV40) and papillomavirus ... See full document
9
Identification of a novel antiapoptotic functional domain in simian virus 40 large T antigen.
... for DNA tumor virus studies ...small t antigen, respectively), or dlA2433, a mutant with a misfolded carboxyl half due to a deletion of aa 587 to 589 ...carboxyl-region ... See full document
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Oligomerization and origin DNA-binding activity of simian virus 40 large T antigen.
... In contrast, studies carried out with lytic T-antigen subclasses separated by zone velocity sedimentation or phosphocellulose chromatography showed that newly synthesized 5 to 7S T antig[r] ... See full document
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Simian Virus 40 Large T Antigen Can Specifically Unwind the Central Palindrome at the Origin of DNA Replication
... of simian virus 40 (SV40) large T antigen play a critical role in DNA ...normal DNA binding and ATPase and helicase activities but with a severely reduced ... See full document
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DNA-binding activity of simian virus 40 large T antigen correlates with a distinct phosphorylation state.
... i They clearly show the relationship between different subclasses, i.e., the DNAbinding, monomeric nuclear high-salt fractions on one hand and the nonbinding, oligomeric fractions on the[r] ... See full document
12
Simian virus 40 small t antigen activates the carboxyl-terminal transforming p53-binding domain of large T antigen.
... small t helper function in SV40 transforma- tion and metabolic stabilization of p53 in complex with large ...with large T actively contributes to phenotypic trans- formation by SV40, both ... See full document
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Four major sequence elements of simian virus 40 large T antigen coordinate its specific and nonspecific DNA binding.
... By testing the effects of single-site mutations on virus replication and by performing a number of DNA-binding assays on replication-negative T-antigen mutants, we have identified severa[r] ... See full document
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Antigenic binding sites of monoclonal antibodies specific for simian virus 40 large T antigen.
... Nine monoclonal antibodies reacted with determinants located in region I, because the antibodies precipitated small t as well as large T antigens from extracts of SV40-transformed or -in[r] ... See full document
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Structure-Based Analysis of the Interaction between the Simian Virus 40 T-Antigen Origin Binding Domain and Single-Stranded DNA
... the T-Ag OBD and its interactions with ...the T-Ag OBD by nuclear magnetic resonance (NMR) meth- ods (30) and established that it is a monomer in ...crystallography studies, we determined the ... See full document
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Simian virus 40 origin DNA-binding domain on large T antigen.
... The variants used to map the DNA-binding domain include: i fragments of large T produced by in vitro translation of unspliced SV40 cRNA 42; ii various truncated forms of large T present [r] ... See full document
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Specific mutation of a regulatory site within the ATP-binding region of simian virus 40 large T antigen.
... Although we already knew that the SV2905 genome was defective for viral DNA replication in vivo, we tested the purified 2905T in an SV40 DNA replication assay in vitro 48.. Our assay was[r] ... See full document
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Biochemical analysis of mutants with changes in the origin-binding domain of simian virus 40 tumor antigen.
... These activities include origin-specific and nonspecific DNA binding, melting of the imperfect palindromic sequence, untwisting of the AT-rich region, unwinding of origincontaining DNA, [r] ... See full document
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Analyses of the Interaction between the Origin Binding Domain from Simian Virus 40 T Antigen and Single-Stranded DNA Provide Insights into DNA Unwinding and Initiation of DNA Replication
... the origin. A model of the T-ag OBD spiral hexamer indicating the positions of the A1 and B2 ele- ments is shown in ...the T-ag OBD with DNA is de- picted in ...the T-ag OBD is also ... See full document
12
Role of Single-Stranded DNA Binding Activity of T Antigen in Simian Virus 40 DNA Replication
... single-stranded DNA binding domain of large T antigen to amino acid residues 259 to ...this domain most likely begins after residue 301 and that the region between ... See full document
9
Three domains in the simian virus 40 core origin orchestrate the binding, melting, and DNA helicase activities of T antigen.
... Simian virus 40 large tumor antigen requires three core replication origin domains for DNA unwinding and replication in vitro.. Simian virus 40 SV40 DNA replication: SV40 large T antigen[r] ... See full document
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Identification of simian virus 40 T-antigen residues important for specific and nonspecific binding to DNA and for helicase activity.
... 4858 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest We have previously identified three regions called elements in the DNA-binding domain of simian virus 40 large tumo[r] ... See full document
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Expression of simian virus 40 T antigen in Escherichia coli: localization of T-antigen origin DNA-binding domain to within 129 amino acids.
... However, a description of the precise protein-DNA interactions of T antigen with these two different sites at a level comparable to that of procaryotic DNA-binding proteins 33 requires m[r] ... See full document
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Characterization of the DNA-Binding Properties of the Origin-Binding Domain of Simian Virus 40 Large T Antigen by Fluorescence Anisotropy
... upon binding to DNA, or dimerizes only weakly, but rather binds primarily in an independent manner to each TBS under our assay ...independent binding of two OBD molecules to two adjacent inverted ... See full document
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