[PDF] Top 20 Template switching by reverse transcriptase during DNA synthesis.
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Template switching by reverse transcriptase during DNA synthesis.
... We examined several variables that affected the efficiency of the reaction: i the reaction time, ii the relative amounts of acceptor and donor template, iii the extent of sequence overla[r] ... See full document
8
Mutations in the Primer Grip of Human Immunodeficiency Virus Type 1 Reverse Transcriptase Impair Proviral DNA Synthesis and Virion Maturation
... This report describes the effects of mutating highly conserved residues in the primer grip domain of human immunodeficiency virus type 1 reverse transcriptase (RT) on virus formation and infectivity. Among ... See full document
5
Base Pairing between cis-Acting Sequences Contributes to Template Switching during Plus-Strand DNA Synthesis in Human Hepatitis B Virus
... plus-strand template switches, how this base pairing contributes is unclear. During template switching, several events must ...tertiary DNA conformation that juxtaposed the donor and ... See full document
9
Relative Rates of Retroviral Reverse Transcriptase Template Switching during RNA- and DNA-Dependent DNA Synthesis
... plate switching during RNA- and DNA-dependent DNA syn- ...vector during either RNA- or DNA- dependent DNA synthesis was expected to result in viral DNA that ... See full document
9
The M184V Mutation in the Reverse Transcriptase of Human Immunodeficiency Virus Type 1 Impairs Rescue of Chain-Terminated DNA Synthesis
... coded reverse transcriptase (RT) and protease enzymes (9, 12, 13, ...kinases. DNA synthesis is blocked once the chain terminator is incorporated, since the nucleoside analog lacks a 3⬘-OH ... See full document
7
Structural Determinants of Murine Leukemia Virus Reverse Transcriptase That Affect the Frequency of Template Switching
... RT template switching for RNase H mutation Y598V suggests that decreasing the rate of DNA polymeriza- tion is unable to overcome this particular defect in RNase H ...the ... See full document
8
Human Immunodeficiency Virus Type 1 Central DNA Flap: Dynamic Terminal Product of Plus-Strand Displacement DNA Synthesis Catalyzed by Reverse Transcriptase Assisted by Nucleocapsid Protein
... the reverse transcriptase (RT)-associated RNase H (37, 38, ...plus-strand synthesis (4, 5, 21, 22, ...catalyzed DNA elongation, whereas RT only pauses at this stop signal in absence of SD ... See full document
13
Correlated Template-Switching Events during Minus-Strand DNA Synthesis: a Mechanism for High Negative Interference during Retroviral Recombination
... one template switch, whereas each plus-strand recombination event results in two template ...one template switches were previously ...one template switch were generated by minus-strand ... See full document
9
Quantification of the Effects on Viral DNA Synthesis of Reverse Transcriptase Mutations Conferring Human Immunodeficiency Virus Type 1 Resistance to Nucleoside Analogues
... (HIV-1) reverse transcriptase (RT) resistance mutations reduce the susceptibility of the virus to nucleoside analogues but may also impair viral DNA ...HIV-1 DNA synthesis and to ... See full document
11
Effects on DNA synthesis and translocation caused by mutations in the RNase H domain of Moloney murine leukemia virus reverse transcriptase.
... strong-stop DNA in vivo with wild-type virus, implying that this species does not normally accumulate in the cell, but rather is efficiently trans- located and ...strong-stop DNA detected in vitro ... See full document
13
Incomplete removal of the RNA primer for minus-strand DNA synthesis by human immunodeficiency virus type 1 reverse transcriptase.
... For the experiments in which the fate of the RNA primer was determined, the T348 fragment was excised from the single-stranded vector with EcoRI as previously described 5; the resulting [r] ... See full document
7
Equine infectious anemia virus and human immunodeficiency virus DNA synthesis in vitro: characterization of the endogenous reverse transcriptase reaction.
... 1952 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The endogenous reverse transcriptase reaction of equine infectious anemia virus EIAV has been studied, and conditio[r] ... See full document
8
Initiation of DNA Synthesis by the Avian Retrovirus Reverse Transcriptase In Vitro: Nature and Location of the Oligodeoxycytidylic Acid Primer Binding Site
... In this report we present data indicating that an additional exogenous synthetic homopolymer, deoxycytidylic acid [oligodC], can also serve as a primer on the avian sarcoma virus ASV gen[r] ... See full document
8
Cryptic Protein Priming Sites in Two Different Domains of Duck Hepatitis B Virus Reverse Transcriptase for Initiating DNA Synthesis In Vitro
... subsequent template switch from ε to the 3 ⬘ end of pgRNA to continue minus-strand DNA synthesis, which normally oc- curs following protein priming (45, ...protein-primed DNA or RNA ... See full document
12
Effects of zidovudine-selected human immunodeficiency virus type 1 reverse transcriptase amino acid substitutions on processive DNA synthesis and viral replication.
... the reverse transcriptase (RT), including D67N, K70R, T215Y, and K219Q, cause high-level resistance of human immunodeficiency virus type 1 (HIV-1) to zidovudine (3 * - azidothymidine; AZT) and appear to ... See full document
8
Site-specific RNA binding by a hepatitis B virus reverse transcriptase initiates two distinct reactions: RNA packaging and DNA synthesis.
... 32p linkage clearly results from the polymerase activity of P protein, since DHBV E is unable to mediate DNA priming when the conserved reverse transcriptase motif YMDD of DHBV polymeras[r] ... See full document
9
Involvement of retrovirus reverse transcriptase-associated RNase H in the initiation of strong-stop (+) DNA synthesis and the generation of the long terminal repeat.
... The fact that strongstop + DNA obtained from reconstructed reactions containing purified reverse transcriptase also contains these oligoribonucleotides suggests that one of the enzymatic[r] ... See full document
9
Effect of Distance between Homologous Sequences and 3′ Homology on the Frequency of Retroviral Reverse Transcriptase Template Switching
... of reverse transcrip- tase (RT) template ...of template switching was greater within the 5 ⴕ regions in comparison to the 3 ⴕ ...RT switching templates within the 5 ⴕ regions doubled ... See full document
10
TP-RT Domain Interactions of Duck Hepatitis B Virus Reverse Transcriptase in cis and in trans during Protein-Primed Initiation of DNA Synthesis In Vitro
... conformation during trans-priming initiation is similar to that during polymerization of cis-priming and that both trans-priming and polymerization occur following cis-priming initiation, which induces the ... See full document
15
Template Switching During Break-Induced Replication Is Promoted by the Mph1 Helicase in Saccharomyces cerevisiae
... prime DNA synthesis, templated by the donor ...subpathways: synthesis-dependent strand annealing (SDSA), canonical double-strand break repair (DSBR), or break-in- duced replication ...(BIR). ... See full document
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