[PDF] Top 20 Unwinding of Parental Strands During Simian Virus 40 DNA Replication

... Isopycnic banding in CsCl-ethidium bromide and sedimentation velocity studies in the presence of various amounts of ethidium bromide indicate that these structures contain negative super[r] ... See full document

... single-stranded DNA binding domain of large T antigen to amino acid residues 259 to ...support simian virus 40 (SV40) replication and to bind to single-stranded ...Two ... See full document

... duplex DNA enters the central channel, and then at least one strand transits to the helicase surface via the side channels (23, 29, 44, ...for DNA replication, may serve the analogous function of ... See full document

... synthetic replication Y fork in the presence of ATP ...fork DNA consisted of a 25-bp duplex region and two 30-nt ssDNA ...origin unwinding but are not directly involved in nonspecific helicase or ... See full document

... the DNA binding domain (residues 131 to 260) determined by nuclear magnetic resonance spectroscopy (29) reveals that three of the class 4 mutations and the class 5 mutation target a cluster of residues on the ... See full document

... range virus defects have been reported at several distinct stages of the viral life cycle in restrictive cells, including early and late gene expression (22–24), DNA replication (18), and virion ... See full document

... 66, 1992 ROLE OF T ANTIGEN AS A REPLICATIVE DNA HELICASE the viral origin of DNA replication, a bilobed T-antigen helicase complex was observed operating simultaneously at both forks of [r] ... See full document

... SSB-coated DNA did not re- lieve the inhibition of primer synthesis and elongation (data not shown), in agreement with previous work ...the DNA unwinding defect of the 5030 T antigen may be primarily ... See full document

... bulk DNA, an im- proved version of a hydroxyapatite thermochromatography method was used, as described previously (53), to separate short-chain replication intermediates from bulk DNA in Ehrlich ... See full document

... Indeed, SV40 RI molecules obtained from unirradiated infected cells present such gaps close to replication forks when they are analyzed by electron microscopy Fig.. Using the standard sp[r] ... See full document

... If the gaps in the pulse-labeled pDNA II are localized in a specific region, then cleavage of the newly synthesized strands by RI endonuclease should yield two populations of labeled fra[r] ... See full document

... Ongoing viral DNA replication panels d and e led to a continuous increase in nuclear matrix-associated SV40 minichromosomes, but a significantly higher proportion of SV40 minichromosomes[r] ... See full document

... Using ecoRI and hpaII restriction endonucleases, each of which can cleave SV40 DNA once at a known location 10, 11, 12, 14, the bound protein can be localized at 0.7 i 0.05 on the SV40 D[r] ... See full document

... However, we demonstrate that mouse DNA sequences present in the SV40 late region of the rescued clone and derived from sequences flanking the SV40 integration site in VLM DNA suppress re[r] ... See full document

... 411 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Various monoclonal antibodies specific for simian virus 40 large tumor antigen T antigen inhibit the elongation proc[r] ... See full document

... When the level of polymerase activity and the ratio of early to late strand transcripts synthesized in vitro are compared with levels and types of RNA that are found in vivo, the two agr[r] ... See full document

... The studies described above indicated that the semipermissive nature of infection of early passage human fibroblasts with SV40 is consistent with a readily detectable level of viral DNA [r] ... See full document

... Footprint and fragment assays led to the following conclusions: i in some cases a single recognition pentanucleotide is sufficient to direct the binding and accurate alignment of A prote[r] ... See full document

... This dramatic inhibition by small t depends on the presence of large T during the preincubation period; when extracts were preincubated with small t alone and large T was added at the be[r] ... See full document

... The large tumor antigen T antigen of simian virus 40 SV40 is a multifunctional regulatory protein that is required for a number of steps in productive viral infection, including DNA repl[r] ... See full document