[PDF] Top 20 Validation of a Multiplex Pneumococcal Serotyping Assay with Clinical Samples
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Validation of a Multiplex Pneumococcal Serotyping Assay with Clinical Samples
... multibead assay is well suited for large-scale epidemio- logic studies because the assay is simple, reliable, and ...stored samples and the existence of original data significantly help any fol- ... See full document
6
Bead Based Multiplex Genotyping of 58 Cutaneous Human Papillomavirus Types
... and validation of a novel bead-based multiplex genotyping assay, McPG, that allows for the simultaneous detection of 38 defined HPV types in the beta, gamma, mu, and nu PV genera, as well as of 20 FA ... See full document
8
Utility of a Multiplex PCR Assay for Detecting Herpesvirus DNA in Clinical Samples
... The multiplex herpesvirus PCR that we describe resulted in increased detection rates for HSV-1, HSV-2, CMV, and VZV compared to those for previously used methods for detection of these ...the validation ... See full document
5
Sequential multiplex PCR assay for determining capsular serotypes of colonizing S pneumoniae
... PCR-based serotyping developments [22]. A first sequential multi- plex PCR assay specific to invasive pneumococcal dis- eases (IPD) distribution in the USA was developed by Pai and colleagues ... See full document
9
Rapid Multiplex Assay for Serotyping Pneumococci with Monoclonal and Polyclonal Antibodies
... semiautomated pneumococcal serotyping system incorporating a pneumococcal lysate preparation protocol and a multiplex serotyping ...confirm pneumococcal identification that also ... See full document
7
Development of a TaqMan Array Card for Pneumococcal Serotyping on Isolates and Nasopharyngeal Samples
... lytA assay were tested against 54 serotyped pneumococcal isolates as well as several nonpneumococcal species, and 100% specificity with no cross-reactivity was observed (see ...primer/probe assay was ... See full document
9
Development of a Multiplex PCR Assay for Rapid Molecular Serotyping of Haemophilus parasuis
... current serotyping scheme, based on reactions between antisera and surface antigens, classi- fies the bacteria into 15 serovars, with a considerable number of nontypeable (NT) isolates observed (22, ...or ... See full document
10
Development and Validation of a Fourfold Multiplexed Opsonization Assay (MOPA4) for Pneumococcal Antibodies
... serum samples (5 prevaccination, 25 postvaccination) were tested in the sin- gle-serotype OPA format and the MOPA4 format ...7F assay by retesting the six ...to assay inaccuracy but rather random ... See full document
6
Direct Detection and Prediction of All Pneumococcal Serogroups by Target Enrichment Based Next Generation Sequencing
... microarray assay differentiating 22 serotypes and 24 other serotypes to the subgroup level, the sequetyping method, which relies on sequencing products of a single consensus pair of primers capable of amplifying ... See full document
9
Impact of Analytical Variability on Clinical Interpretation of Multiplex Pneumococcal Serology Assays
... testing. Samples were tested with three dif- ferent multiplex assays for the measurement of serotype-specific pneumo- coccal IgG ...antibodies. Samples were tested on site at the Cleveland Clinic ... See full document
5
Validation of a New Aspergillus Real Time PCR Assay for Direct Detection of Aspergillus and Azole Resistance of Aspergillus fumigatus on Bronchoalveolar Lavage Fluid
... the validation of the AsperGenius, a new multiplex real-time PCR assay consisting of two multiplex real-time PCRs, one that identifies the clinically relevant Aspergillus species, and one that ... See full document
7
Competitive Inhibition Flow Analysis Assay for the Non-Culture-Based Detection and Serotyping of Pneumococcal Capsular Polysaccharide
... and pneumococcal suspensions, the serotype results of which were compared to the serotypes determined by the com- petitive inhibition ...inhibition assay in the two sam- ple types differed from each other ... See full document
8
Detection and Serotyping of Dengue Virus in Serum Samples by Multiplex Reverse Transcriptase PCR Ligase Detection Reaction Assay
... the multiplex capability of the PCR-LDR assay. A multiplex PCR amplification of both target regions used all 46 PCR primers in a single ...a multiplex ligation reaction using the 75 primers ... See full document
9
Accuracy of High Throughput Nanofluidic PCR Based Pneumococcal Serotyping and Quantification Assays Using Sputum Samples for Diagnosing Vaccine Serotype Pneumococcal Pneumonia: Analyses by Composite Diagnostic Standards and Bayesian Latent Class Models
... serotype pneumococcal pneumonia (VTPP) remains a challenging issue in pneumococcal vaccine ...diagnosing pneumococcal bacteremia, and the proportion of bacteremia cases among PP cases is only 5 to ... See full document
10
From Quellung to Multiplex PCR, and Back When Needed, in Pneumococcal Serotyping
... of serotyping for surveillance and vaccine efficacy ...For validation, 170 isolates were serotyped using the new protocol with six sequential multiplex PCRs for the deduction of serotypes, ... See full document
5
Challenges of Using Molecular Serotyping for Surveillance of Pneumococcal Disease
... PCR assay (10) or a multiplex real-time PCR assay (22) as previously ...singleplex assay, each 25- l reaction consisted of 1 ⫻ TaqMan gene expression master mix (Applied Biosystems, Foster ... See full document
6
Accuracy of a Polymerase Chain Reaction-based Assay for Detection of Pneumococcal Bacteremia in Children
... the clinical trial which have crossreacted with this ...the clinical infor- mation we gathered showed that patients who were PCR positive and culture negative did not possess predictors classically ... See full document
6
ELISA in the multiplex era: potentials and pitfalls
... procedures, assay imprecision (CV), analyte recovery and assay linearity (LOQs) ...reference samples of low, medium and high analyte concentrations at the commencement of a period of usage ...a ... See full document
17
Host chemokine signature as a biomarker for the detection of pre-cancerous cervical lesions
... the samples from women with CIN3 vs no ...disease. Validation of these findings was performed on a multiplex Bioplex assay which showed that CCL2, CCL3, CXCL1, CXCL8 and CXCL12 were ... See full document
11
Development of a Multiplex PCR Assay Targeting O Antigen Modification Genes for Molecular Serotyping of Shigella flexneri
... A multiplex PCR showed that the serotype Y and X isolates were originally 2a and 2b, re- spectively, while the serotype 5a strain (NCTC8523) was orig- inally a novel F5 ... See full document
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