ANDHERI / BORIVALI / DADAR / CHEMBUR / THANE / MULUND/ NERUL / POWAI TOPIC:GENETIC ENGINEERING AND GENOMICS
1. Chromosomes are made up of __________ .
(a) DNA (b) RNA (c) DNA and RNA (d) ribosome
2. The technique developed to identify a person with the help of DNA restriction analysis is known as __________ .
(a) DNA profiling (b) DNA fingerprinting (c) Tissue culture (d) Both (a) and (b) 3. VNTR is __________ .
(a) Variable Number of Thymine Repeats (b) Variable Number of Transcription Repeats (c) Variable Number of Tandem Repeats (d) Variable Nucleotide of Tandem Repeats 4. Transfer of DNA bands from agarose gel to nitrocellulose or nylon membrane is __________ . (a) Southern blotting (b) Western blotting (c) Northern blotting (d) Eastern blotting 5. What is the full form of RFLP?
(a) Restriction Fragment Length Polymerase (b) Restricted Fragment Length Polymorphism (c) Restriction Fragment Length Polymorphism (d) Regular Fragment Length Polymerase 6. Human genome project was launched in __________ .
(a) 1986 (b) 1990 (c) 1996 (d) 1998
7. The human genome project involved sequence of __________ .
(a) 30,000 base pairs (b) 3 billion base pairs (c) 2000 base pairs (d) 450 base pairs 8. Physical maps specify __________ .
(a) distance between genes and markers on the chromosomes (b) the physical location of genes
(c) distance between genes or DNA fragments (d) both (b) and (c)
9. The genome size of Yeast is __________ .
(a) 1830 kb (b) 1660 kb (c) 12500 kb (d) 569 kb
10. A vaccine is an __________ used to induce immunity against several diseases.
(a) antibody preparation (b) antigenic preparation (c) adjuvant preparation (d) chemical preparation
CENTERS: MUMBAI / DELHI / AKOLA / KOLKATA / LUCKNOW / NASHIK / GOA # 3
11. Adjuvant is added to a dilute mixture during production of vaccine to __________ .(a) improve the immune response to the toxoid (b) improve the penetration of vaccine in the cells
(c) help the vaccine to react specifically with diseased cells (d) improve the immunity
12. Mice are the approximate choice for transgenesis because __________ .
(a) they have short generation time (b) a superovulated mouse can yield upto 40 eggs.
(c) reimplantation is relatively easier (d) all of the above 13. Which property of transgenic animals is used in chemical safety testing?
(a) Insensitivity to toxic substance (b) Sensitivity to toxic substance (c) Resistance to toxic substance (d) Both (a) and (c)
14. HGP explored a new field in biology called __________ .
(a) Genome (b) Genomics (c) Genetics (d) all of these
15. People who do not produce the necessary amount of insulin suffer from a disease called _________ . (a) diabetes insipidus (b) diabetes mellitus (c) juvenile diabetes (d) none of the above 16. VNTRs is the key factor in DNA profiling because __________ .
(a) the length of the regions having VNTRs is different in each individual.
(b) the length of the regions having VNTRs is same in each individual.
(c) they have nucleotide
(d) short pieces of nucleotides are same in all persons.
17. DNA probes used in finger printing are __________ .
(a) highly sensitive electron microscope (b) UV beams (c) DNA segments having radioactive isotopes (d) X-ray scanners
18. DNA sample hybridized with DNA probe when kept in contact with X-ray film results into __________ .
(a) photographic image of radioactive probe
(b) photographic image of DNA bands due to radioactive probe (c) photographic image of nylon membrane
(d) both (a) and (b)
19. An immediate impact of HGP was __________ .
(a) identification of genes associated with human diseases (b) development of new strategies for disease detection.
(c) development of innovative therapies for treatment of disease (d) all of the above
CENTERS: MUMBAI / DELHI / AKOLA / KOLKATA / LUCKNOW / NASHIK / GOA # 4
20. Which of the following statements regarding HGP is incorrect?(a) HGP is involved in maping and sequencing of model organisms – E. coli and Mus musculus.
(b) HGP is involved in finding the genes related to human disease in Saccharomyces cerevisiae (c) HGP is involved in study of gene function of model organisms
(d) HGP is involved in greater understanding of human evolution by studying the genes in flies, roundworms and mice.
21. Which statement about insulin is correct?
(i) Raised blood glucose level triggers cells of islet of Langerhans to release insulin.
(ii) Decreased blood glucose level triggers cells of islet of Langerhans to release insulin.
(iii) Binding of insulin to receptors on the cell’s membrane activates the transport of glucose and protein.
(iv) Without insulin, the glucose builds up in the blood and the cells are starved of their energy source.
(a) (i) and (ii) are correct (b) (i), (iii) and (iv) are correct (c) (i), (ii), (iii) are correct (d) (ii) and (iv) are correct
22. Insulin extracted and purified from the pancreas of cattle and pigs functions well in the human body because __________ .
(a) the chemical structure of insulin in these animals is slightly different from human insulin.
(b) the structure of cells of pancreas of these animals and human are same.
(c) both (a) and (b) (d) none of these
23. Vaccine production involves __________ . (a) growth of organisms on culture medium.
(b) extraction of the toxin from cultured organism and altering it to develop into toxoid.
(c) mixing the toxoid with adjuvant.
(d) all of the above.
24. Gene therapy involves __________ .
(a) deletion or replacement of defective gene
(b) introduction of desired gene which is not found in an organism.
(c) modification of germ-line cells to correct a genetic defect.
(d) all of the above
25. Which one of the following pairs is not correctly matched?
(a) Tissue plasminogen activator – prevents or reverses blood clots.
(b) DNAse – treats cystic fibrosis.
(c) Bovine growth hormone – treats hepatitis B
(d) Human Insulin (HUMULIN) – treats diabetes mellitus
26. Which of the following is specifically used in genetic engineering?
(a) Ligase (b) Gyrase
(c) Restriction endonuclease (d) DNA polymerase
CENTERS: MUMBAI / DELHI / AKOLA / KOLKATA / LUCKNOW / NASHIK / GOA # 5
27. Polymerase Chain Reaction is most useful in _________ .(a) DNA synthesis (b) DNA amplification (c) protein synthesis (d) amino acid synthesis 28. Variable Number Tandem Repeats in the DNA molecule are highly useful in _________.
(a) monoclonal antibody production (b) stem cell culture (c) recombinant DNA technology (d) DNA fngerprinting 29. The key factor in DNA profiling is length of __________ .
(a) cistron (b) mutons (c) polypeptide (d) VNTRs
30. Paper used for southern blotting in DNA fingerprinting is __________ .
(a) litmus (b) Whatman (c) nitrocellulose (d) cellulose
31. In India, the radioactive DNA probe is obtained form banded krait. In krait, the sex chromosome is _______ .
(a) XX-XY (b) ZZ-ZW (c) XY-YY (d) ZW-WW
32. Which genetic map is most detailed?
(a) Cytogenetic (b) Linkage (c) Physical (d) Sequence 33. The best animal for gentic engineering experiments is __________ .
(a) pig (b) sheep (c) mouse (d) fish
34. Differences between parents and offsprings occur due to __________ . (a) replication of DNA (b) protein synthesis (c) recombination of DNA (d) mitosis
35. Technique developed to identify a person with the help of DNA is called ________ . (a) genomics (b) fingerprinting (c) genetics (d) HGP 36. What percentage of nucleotides sequence is same in all persons?
(a) 100 (b) 99 (c) 98 (d) 97
37. In a population, number of unusual sequences in every person is from __________ . (a) 20-100 base pairs (b) more than 100 base pairs (c) less than 100 base pairs (d) no such occurrence 38. The unusual sequences that are repeated several times are called __________ .
(a) VNTRs (b) RFLP (c) PCR (d) HGP
39. Another term describing DNA fingerprinting is ___________ .
(a) DNA profiling (b) HGP (c) VNTR (d) PCR
40. DNA fingerprinting process begins with ___________ .
(a) isolation of DNA (b) amplification (c) fragmentation (d) electrophoresis 41. Cry gene produces a protein that functions as ___________ .
(a) pesticide (b) fungicide (c) weedicide (d) bactericide
CENTERS: MUMBAI / DELHI / AKOLA / KOLKATA / LUCKNOW / NASHIK / GOA # 6
42. In the mature insulin, ___________ does not occur.(a) A peptide (b) B peptide (c) C peptide (d) D peptide 43. On which chromosome is the gene for Huntington’s disease located?
(a) 2 (b) 11 (c) 4 (d) 22
44. In early treatment of diabetes, insulin was extracted from pancreas of slaughtered _________ .
(a) cattle (b) dog (c) horse (d) chick
45. Chitinase is used in the treatment of ____________ .
(a) bacteria (b) virus (c) plant (d) fungus
46. During genetic engineering, bacterial cell is treated by _____________ .
(a) lysozyme (b) cellulase (c) chitinase (d) gyrase 47. The two chains of insulin are linked by __________ .
(a) phosphorous (b) disulphide (c) hydrolase (d) nitrogenous base 48. Southern blotting of DNA strands during DNA fingerprinting is on _________ .
(a) Whatman paper (b) cellulose paper (c) nylon (d) graph paper 49. In DNA fingerprinting, the DNA probe is __________ .
(a) heavy isotope (b) radioactive (c) ordinary (d) with higher density 50. In Southern blotting technique, __________ remains on nylon sheet.
(a) single stranded DNA (b) single stranded probe DNA (c) double stranded hybridized DNA (d) double stranded probe DNA 51. The nylon membrane of Southern blotting is kept in contact with __________ .
(a) X-ray film (b) agarose gel (c) nitrocellulose paper (d) cellulose paper 52. In India, Dr. Lalji Singh obtained the radioactive DNA probe from __________ .
(a) mouse (b) guinea pig (c) snake (d) bacteria
53. One of the following does NOT indicate the genome of an organis
(a) sperm (b) ovum (c) microspore (d) zygote
54. HGP was initiated and completed in __________ .
(a) 1990, 2003 (b) 1995, 2000 (c) 2000, 2006 (d) 2005, 2009 55. Molecular scissors or chemical scalpels used in genetic engineering is _________ .
(a) ligase (b) endonuclease (c) polymerase (d) gyrase 56. The number of types of maps generated in HGP are _________ .
(a) 4 (b) 3 (c) 2 (d) 1
CENTERS: MUMBAI / DELHI / AKOLA / KOLKATA / LUCKNOW / NASHIK / GOA # 7
57. Genetic linkage map determines __________ .(a) physical location of base pair
(b) distance between genes with unknown function (c) distance between DNA fragments
(d) relative arrangement and approximate distances between genes 58. Physical map of Human Genome Project specifies ________ .
(a) distance between genes with unknown function
(b) determines relative arrangement and approximate distances between genes (c) distance between DNA fragments
(d) markers on the chromosomes
59. The organism Arabidopsis thaliana used in mapping and sequencing of genome is ________ . (a) angiosperm (b) gymnosperm (c) algae (d) fungi
60. Insulin is a hormone that regulates ___________ in human blood.
(a) fructose (b) galactose (c) mannose (d) glucose 61. Raised blood sugar triggers the cell of __________ to release insulin.
(a) Kupffer cells (b) Sertoli cells (c) islets of Langerhans (d) osteocytes 62. When glucose molecule builds up in the blood, the cell is deprived of ___________ .
(a) proteins (b) amino acid (c) energy (d) enzyme 63. Most current gene therapy trials target ____________ .
(a) SCID deficiency (b) HIV (c) cancer (d) cystic fibrosis 64. Genetic engineering manipulates gene products at the level of ____________ .
(a) DNA (b) RNA (c) amino acid (d) protein
65. The first transgenic cow is __________ .
(a) Dolly (b) Molly (c) Rosie (d) Sandy
ANDHERI / BORIVALI / DADAR / CHEMBUR / THANE / MULUND/ NERUL / POWAI
TOPIC: GENETIC ENGINEERING AND GENOMICS (ANSWER KEY)
1. (a) 2. (d) 3. (c) 4. (a) 5. (c)
6. (b) 7. (b) 8. (d) 9. (c) 10. (b)
11. (a) 12. (d) 13. (b) 14. (b) 15. (b)
16. (a) 17. (c) 18. (b) 19. (d) 20. (b)
21. (b) 22. (a) 23. (d) 24. (d) 25. (c)
26. (c) 27. (b) 28. (d) 29. (d) 30. (c)
31. (b) 32. (d) 33. (c) 34. (c) 35. (b)
36. (b) 37. (a) 38. (a) 39. (a) 40. (a)
41. (a) 42. (c) 43. (c) 44. (a) 45. (d)
46. (a) 47. (b) 48. (c) 49. (b) 50. (c)
51. (a) 52. (c) 53. (d) 54. (a) 55. (b)
56. (c) 57. (d) 58. (d) 59. (a) 60. (d)
61. (c) 62. (c) 63. (c) 64. (a) 65. (c)
ANDHERI / BORIVALI / DADAR / CHEMBUR / THANE / MULUND/ NERUL / POWAI