adeno-associated virus 9
Adeno-Associated Virus 9-Mediated Airway Expression of Antibody Protects Old and Immunodeficient Mice against Influenza Virus
... In this study, we demonstrated that, irrespective of their im- mune status and age, vaccinated mice survived the challenge and exhibited no signs of behavioral distress or loss of weight. The immune system of old mice is ...
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Mapping an Adeno-associated Virus 9-Specific Neutralizing Epitope To Develop Next-Generation Gene Delivery Vectors
... ABSTRACT Recent clinical trials have demonstrated the potential of adeno- associated virus (AAV)-based vectors for treating rare diseases. However, significant barriers remain for the translation of ...
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Differential myofiber-type transduction preference of adeno-associated virus serotypes 6 and 9
... AAV: adeno-associated virus; ARRIVE: Animal Research: Reporting of In Vivo Experiments; CMV: cytomegalovirus; CSA: cross-sectional area; CT: cycle of threshold; DAPI: 4 ′ ,6-diamidino-2-phenylindole; ...
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Charged-to-Alanine Scanning Mutagenesis of the N-Terminal Half of Adeno-Associated Virus Type 2 Rep78 Protein
... Fig. 9]), except for E66, which showed weak binding and was negative for endonuclease activity and positive for ability to introduce the ITR plasmid into ...
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A survey of ex vivo/in vitro transduction efficiency of mammalian primary cells and cell lines with Nine natural adeno associated virus (AAV1 9) and one engineered adeno associated virus serotype
... To analyze the tropism of nine different natural AAV se- rotypes (1-9) and one engineered serotype (1.3) (a hybrid of AAV1 and AAV6), we used scAAV vectors that expressed eGFP from the CMV promoter (Figure 1). ...
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Determination of adeno-associated virus Rep68 and Rep78 binding sites by random sequence oligonucleotide selection.
... nine (G) residues. To test the ability of runs of G residues to stabilize the binding of Rep78 to DNA, a probe which con- tained GAGC GGGGGCG in place of the block of 20 random bases was constructed (Fig. 2D). This ...
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Mutational Analysis of Adeno-Associated Virus Type 2 Rep68 Protein Endonuclease Activity on Partially Single-Stranded Substrates
... strate. Care was taken in the design of the D40A-D42A-D44A, E83A-K84A-E86A, and K146A-D149A-E150A mutations to minimize the possibility of inducing global misfolding of the proteins (2, 9). These proteins were ...
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Adeno-associated virus vector integration junctions.
... vector proviruses also contain flanking chromosomal DNA, and their expected sizes can be predicted from the Southern analysis in Fig. 2B. Ligation at low DNA concentration en- hances circularization of individual EcoRI ...
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The 37/67-Kilodalton Laminin Receptor Is a Receptor for Adeno-Associated Virus Serotypes 8, 2, 3, and 9
... Thus far, AAV2 has been the primary serotype tested in clinical trials, but many alternative serotypes that offer specific advantages for certain diseases are currently in preclinical de- velopment. Among these, perhaps ...
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The Adeno-Associated Virus Type 2 Regulatory Proteins Rep78 and Rep68 Interact with the Transcriptional Coactivator PC4
... As stated above, the mutational analysis points to a possible role of Rep-PC4 interactions in the inhibition of HSV-induced SV40 DNA amplification. A function for PC4 in SV40 DNA replication was suggested due to the ...
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SRSF1-dependent nuclear export of C9ORF72 repeat-transcripts: targeting toxic gain-of-functions induced by protein sequestration as a selective therapeutic strategy for neuroprotection
... AAV: adeno-associated virus; ALS: amyotrophic lateral sclerosis; ALYREF: Aly/RNA export factor; AREX: alternative RNA export pathway; C4G2: CCCCGG; C9ORF72: chromosome 9 open reading frame 72; ...
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Factors Influencing Adeno-Associated Virus-Mediated Gene Transfer to Human Cystic Fibrosis Airway Epithelial Cells: Comparison with Adenovirus Vectors
... viruses, adeno-associated virus (AAV) and ade- novirus (Ad), are currently being evaluated as vectors for hu- man gene therapy of cystic fibrosis (CF) (9, 14, 15, 27–29, ...endogenous ...
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Glycan Binding Avidity Determines the Systemic Fate of Adeno-Associated Virus Type 9
... The liver is a prominent systemic organ featuring hepatocytes, as well as a large proportion of cells belonging to the reticuloendo- thelial system. Due to the large volume of circulating blood that filters through the ...
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Cloning and Characterization of Adeno-Associated Virus Type 5
... of virus, but a combi- nation of the 2ITR with the 5RepCapA expression plasmid or the 5ITR with the 2RepCap expression plasmid produced no detectable transduction ...
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Herpes Simplex Virus Type 1/Adeno-Associated Virus Hybrid Vectors Mediate Site-Specific Integration at the Adeno-Associated Virus Preintegration Site, AAVS1, on Human Chromosome 19
... 8, 9, 11, 12, 13, 14, c, and d and in the Detroit 6 cell line 7374, which is latently infected with wild-type ...8, 9, 12, 13, 14, and d, AAVS1-rearranged bands colocalized with GFP- specific ...8, ...
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Structural Insight into the Unique Properties of Adeno-Associated Virus Serotype 9
... Adeno-associated virus serotype 9 (AAV9) has enhanced capsid-associated tropism for cardiac muscle and the ability to cross the blood-brain barrier compared to other AAV ...
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Substitution of adeno associated virus Rep protein binding and nicking sites with human Chromosome 19 sequences
... Virus DNA replication in plasmid-transfected cells was analyzed by using the Southern blotting procedure. A map of the pSub201(-) vector used to make AAV2 containing two wild type ITRs is shown in Figure 3A. As ...
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Gangliosides Are Essential for Bovine Adeno-Associated Virus Entry
... AAVs have also been reported in other mammalian species, including canines, bovines, ovines, and equines (2). Previously, we reported the cloning of bovine adeno-associated virus (BAAV), which is ...
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Adeno-associated virus-mediated neuroglobin overexpression ameliorates the N-methyl-N-nitrosourea-induced retinal impairments: a novel therapeutic strategy against photoreceptor degeneration
... the perturbed calcium homeostasis and the accumulation of unfolded proteins, could activate ER stress in photoreceptors. In the present study, the mRNA levels of ER stress sensors, including CHOP, caspase-9, and ...
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Selective Cleavage of AAVS1 Substrates by the Adeno-Associated Virus Type 2 Rep68 Protein Is Dependent on Topological and Sequence Constraints
... Our in vitro results also have interesting implications for AAV-2 site-specific integrations in vivo. In contrast with the genome of E. coli, which has a net superhelical density () of ⬇⫺0.05 (supercoils per turn), no ...
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