Allele-specific DNA methylation
Roles of Protein Factors in Regulation of Imprinted Gene Expression
... parental allele-specific DNA methylation in the germline and exhibits posttranslational histone ...differential methylation during gametogenesis and/or do not survive the genome-wide ...
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Allele-specific methylation in the FADS genomic region in DNA from human saliva, CD4+ cells, and total leukocytes
... as DNA methylation or histone modifications can influence gene expression levels ...[22]. DNA methylation is known to alter gene ex- pression, especially when located in critically regulatory ...
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Prediction of smoking by multiplex bisulfite PCR with long amplicons considering allele-specific effects on DNA methylation
... In this work, we described a method, which could be utilised for creation of epigenetic clinical tests based on results of various EWAS. The approach allows for multi- plex bisulfite PCR amplification of multiple targets ...
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Allele-Specific Methylation Occurs at Genetic Variants Associated with Complex Disease
... of methylation status with allele identity by chi-square ...of allele-specific ...based Allele-Specific Methylation (ASM) assay. DNA is MSRE treated (left panels) ...
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Determination of quantitative and site specific DNA methylation of perforinby pyrosequencing
... strand DNA was separated by immobi- lizing the PCR product on streptavidin coated Sepharose High Performance beads (Amersham Biosciences, NJ) and strand separated by denaturation with ...
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Tissue specific patterns of allelically skewed DNA methylation
... relative allele score (RAS) values were gener- ated for all SNPs on the array, which are defined as A/(A C B), where A and B are the intensities of the probes for the 2 alleles of a given ...of DNA ...
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DNA methylation, epialleles and gene regulation: Insights from the honey bee
... intragenic methylation in social insects has been hailed as a prime mover of environmentally-driven organismal plasticity and even as evidence for genomic ...deep allele-specific bisulfite DNA ...
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IMAGE: high powered detection of genetic effects on DNA methylation using integrated methylation QTL mapping and allele specific analysis
... sites, methylation levels measured in the heterozygotes are noisy and often indistinguishable from at least one type of homozygote (often because total read counts are unevenly distributed across ...separating ...
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DAMEfinder: a method to detect differential allele-specific methylation
... of DNA meth- ylation asymmetry on gene expression) modulate the susceptibility of the general population to frequent, multi-factorial diseases affecting specific ...
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rs10732516 polymorphism at the IGF2/H19 locus associates with genotype-specific effects on placental DNA methylation and birth weight of newborns conceived by assisted reproductive technology
... the allele-specific methylation profiles and to confirm the EpiTYPER results as well as genotypes, the CTCF6 at H19 ICR of heterozygous ART-derived placen- tal samples with patG/matA genotype (4 ...
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Mono-allelic retrotransposon insertion addresses epigenetic transcriptional repression in human genome
... the allele quantitation algo- rithm using the PSQ 96 HS ...For allele-specific expres- sion, forward primers: TAGTCAAGTGGTCTAAAT- CATTGC, reverse biotin labeled: TTTAGTATTAGA TCAGGTGC, and sequencing ...
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DNA Methylation Validation Methods: a Coherent Review with Practical Comparison
... for DNA methylation validation: MSRE analysis, pyrosequencing, MS-HRM and ...tained DNA methylation information and consistency across various methylation levels, we consider ...
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Original Article Semaphorin-3F functions as a tumor suppressor in colorectal cancer due to regulation by DNA methylation
... The methylation status of CpG island of all sam- ples was initially screened at SEMA3F gene pro- moter regions by methylation-specific PCR (MSP) as described ...Genomic DNA extracted from ...
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DNA Methyltransferase Activity Assays: Advances and Challenges
... for DNA MTase activity is ...of DNA MTase activ- ity ...by specific binding by ...another specific binding with the antibody that is attached to the ...CpG DNA sequences by cleaving ...
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What Do Studies of Insect Polyphenisms Tell Us about Nutritionally-Triggered Epigenomic Changes and Their Consequences?
... epigenome. DNA methylation, the reversible addition of a methyl group to a cytosine residue in the DNA, is one way in which larvae respond to differences in their ...establishes DNA ...
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Age-related DNA methylation changes are tissue-specific with ELOVL2 promoter methylation as exception
... the DNA methylation level ...gain DNA meth- ylation with time as compared with ...sequencing DNA methylation data of newborn versus a centenarian observed a much higher fraction of CpGs ...
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De novo DNA methylation of endogenous retroviruses is shaped by KRAB ZFPs/KAP1 and ESET
... IAP DNA methylation was measured in an ES cell line stably deleted for the G9a histone methyltransferase (Dong et ...in DNA methylation, whereas Kap1-deleted cells divide only a few times ...
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DNA methylation analysis of phenotype specific stratified Indian population
... of methylation, generally represented as log ratio or (INPUT/IP) or “M- value” (methylation) for a given ...prakriti specific differentially methylated re- ...
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Exploring The Role Of Tet1 In Genomic Imprinting
... for DNA methylation erasure, targeted epigenomic modifiers could be ...a specific genomic locus to alter the chromatin ...bind DNA irrespective of its DNA ...
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Promoter methylation correlates with reduced NDRG2expression in advanced colon tumour
... of DNA from colon cancer cell lines, peripheral blood and frozen tissues of patients was assayed, as reported by Herman et ...methylated DNA (IVD) were used as negative and positive controls, ...of ...
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