Arbitrarily primed PCR
Comparison of ribotyping, arbitrarily primed PCR, and pulsed field gel electrophoresis for molecular typing of Listeria monocytogenes
... Serotyping of L. monocytogenes strains is not very discrimi- natory since almost all strains isolated from humans, foods, and environments belong to a small number of serotypes (11, 12, 22, 25). Other techniques used for ...
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The role of arbitrarily primed PCR in identifying the source of an outbreak of Legionnaires' disease
... An outbreak of community-acquired Legionnaires’ disease (LD) occurred in Providence, R.I., in fall 1993. To find the outbreak source, exposures of 17 case patients were compared to those of 33 matched controls. Case ...
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Comparison of arbitrarily primed PCR with restriction endonuclease and immunoblot analyses for typing Clostridium difficile isolates
... Arbitrarily primed PCR (AP-PCR) was used to genotype 26 clinical isolates of Clostridium difficile previously analyzed by immunoblotting (IB) and 20 isolates typed by restriction endonuclease ...
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PCR ribotyping and arbitrarily primed PCR for typing strains of Clostridium difficile from a Polish maternity hospital
... by PCR amplification of the 16S-23S ribosomal intergenic spacer regions and by arbitrarily primed PCR (AP-PCR) with different primers and combinations ...and PCR- mediated ...
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Characterization of Leptospira isolates from serovar hardjo by ribotyping, arbitrarily primed PCR, and mapped restriction site polymorphisms
... Leptospira serovar hardjo isolates of the hardjoprajitno and hardjobovis genotypes were characterized by ribotyping, arbitrarily primed PCR AP-PCR fingerprinting, and the study of mapped[r] ...
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Comparison of Restriction Enzyme Analysis, Arbitrarily Primed PCR, and Protein Profile Analysis Typing for Epidemiologic Investigation of an Ongoing Clostridium difficileOutbreak
... [PFGE], PCR, arbitrarily primed PCR [AP-PCR], restriction enzyme analysis [REA], plasmid profile analysis, and ribotyping) and nongenomic typing methods (pro- tein profile analysis ...
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Evaluation of Two Commercial Kits and Arbitrarily Primed PCR for Identification and Differentiation ofActinobacillus actinomycetemcomitans,Haemophilus aphrophilus, and Haemophilus paraphrophilus
... and arbitrarily primed PCR (AP-PCR) in the identification and differentiation of the three species from each ...the PCR amplification, the oligonucleotide 5 * -CAGCACCCAC-3 * was used ...
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Rapid identification of pathogenic Leptospira species (Leptospira interrogans, L borgpetersenii, and L kirschneri) with species specific DNA probes produced by arbitrarily primed PCR
... identification. PCR-based strategies were developed to categorize the new species of Leptospira ...in PCR-amplified ribosomal genes allowed reference strains to be grouped in their species, in agreement ...
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Application of an Optimized and Highly Discriminatory Method Based on Arbitrarily Primed PCR for Epidemiologic Analysis of Methicillin Resistant Staphylococcus aureusNosocomial Infections
... By arbitrarily amplifying variable regions in the bacterial genome (arbitrarily primed PCR [AP-PCR]), an isolate-specific DNA fingerprint can be obtained in a rapid and reproducible ...
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Pandemic Spread of an O3:K6 Clone of Vibrio parahaemolyticus and Emergence of Related Strains Evidenced by Arbitrarily Primed PCR and toxRS Sequence Analyses
... unique arbitrarily primed PCR (AP-PCR) profiles ...new PCR method targeted to 2 of the base positions unique to the new O3:K6 clone was ...This PCR method could clearly ...
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Multicenter evaluation of arbitrarily primed PCR for typing of Staphylococcus aureus strains
... Fifty-nine isolates of Staphylococcus aureus and a single strain of Staphylococcus intermedius were typed by arbitrarily primed PCR (AP-PCR). To study reproducibility and discriminatory ...
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Arbitrarily primed PCR, ribotyping, and plasmid pattern analysis applied to investigation of a nosocomial outbreak due to Enterobacter cloacae in a neonatal intensive care unit
... hospitalized in the neonatal ICU [patients A, B, C, E, F, G, T, U, and V], 2 were hospitalized in the pediatric ICU [patients H and S], and 1 was hospitalized in the special baby care un[r] ...
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Incidence of Prevotella intermedia andPrevotella nigrescens Carriage among Family Members with Subclinical Periodontal Disease
... of PCR ribotyping and arbitrarily primed PCR (AP-PCR) fingerprinting and applied this system to the study of intrafamilial incidence of these species in the oral ...cavity. PCR ...
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Arbitrarily primed polymerase chain reaction as a rapid method to differentiate crossed from independent Pseudomonas cepacia infections in cystic fibrosis patients
... We used DNA fingerprinting by the arbitrarily primed polymerase chain reaction AP-PCR technique for an epidemiological investigation of 23 Pseudomonas cepacia isolates obtained from 11 c[r] ...
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T Cell-, Interleukin-12-, and Gamma Interferon-Driven Viral Clearance in Measles Virus-Infected Brain Tissue
... (RT)- PCR, and primers were designed to specifically amplify MV N gene (3 ⬘ -ATGG CCACACTTTTAAGGAGCTTAG-5⬘, 5⬘-CGGTCCAGAAGTCTGGATCGA G-3 ⬘ ) and control hypoxanthine-guanine phosphoribosyltransferase (HPRT) gene ...
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Spinocerebellar ataxia type 36 in the Han Chinese
... Repeat-primed PCR assays and PCR-fragment analysis for the number of short hexanucleotide repeats (,40 units) were performed to ascertain NOP56 hex- anucleotide repeat ...
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Molecular Typing of Salmonella enterica Serovar Typhi Isolates from Various Countries in Asia by a Multiplex PCR Assay on Variable Number Tandem Repeats
... multiplex PCR method incorporating primers flanking three variable-number tandem repeat (VNTR) loci (arbitrarily labeled TR1, TR2, and TR3) in the CT18 strain of Salmonella enterica serovar Typhi has been ...
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Leishmania major: Genetic Profiles of the Parasites Isolated from Chabahar, Southeastern Iran by PPIP-PCR
... Primers used were 2B (5’-CAG GAG CGC GCA CAC GCA CAC ACG -3’) and 2A (5’- ATT AAC CCT CAC TAA ACC CAA CAA - 3’) (4). Amplification reactions were performed in 50 μl containing 25 μl Tag Mas- ter Mix RED (Ampliqon, ...
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The Association of Gender, Age, and Coping with Internalizing Symptoms in Youth with Sickle Cell Disease
... arbitrarily long, random words and the minimum density of an unavoidable word over.. arbitrarily long words.[r] ...
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A new Aura virus isolate in Brazil shows segment duplication in the variable region of the nsP3 gene
... Next, we used an adapted non-specific RT-PCR amplification protocol that was designed to amplify any kind of viral nucleic acid. First, we carried out an enrichment step, which consisted of supernatant fil- ...
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