cell-free gene expression
Stögbauer, Tobias Roland (2012): Experiment and quantitative modeling of cell-free gene expression dynamics. Dissertation, LMU München: Fakultät für Physik
... of cell-free gene expression kinetics based on differ- ential equations for individual kinetics rates is ...way cell-free gene expression kinetics could be fitted ...
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Cell-free soluble expression of the membrane protein PsbS
... PsbS gene from Physcomitrella patens (Krishnan et ...Expressway™ Cell-‐Free Expression System (Invitrogen) was used to carry out the CF reactions We used 25 µl reaction mixture (RM) ...
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Effect of UV irradiation on the expression of vaccinia virus gene products synthesized in a cell-free system coupling transcription and translation.
... In considering the coupling of vacinia mM GTP, 0.5 mM CTP, 200 mCi of r3HJUTP per at a final concentration of 0.1 mM; and 1 OD20 virus-directed RNA synthesis to translation, we 1tmol of [r] ...
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Growth Phase Specific Modulation of Cell Morphology and Gene Expression by an Archaeal Histone Protein
... Histone as a regulator of gene expression in open chromatin. In H. salinarum, histone and other putative NAPs appear to be present in low abundance in both the cytosolic control and the DNA fractions, ...
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<p>Decreased PTGDS Expression Predicting Poor Survival of Endometrial Cancer by Integrating Weighted Gene Co-Expression Network Analysis and Immunohistochemical Validation</p>
... low expression is associated with poor prognosis, includ- ing in breast ...higher expression levels of PTGDS were correlated with longer disease-free survival and higher sensitivity to platinum drugs ...
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E2F-1 has dual roles depending on the cell cycle
... target gene expression in the same cell de- pending on the cell ...Although cell cycle pheno- typic effects of E2F-1 are the same on both cycling cells and cells re-entering the ...
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Cell-free expression systems for the construction of artificial cells
... reporter gene used in this study is a fluorescent ...and expression of an enzyme to be tested in an assay would provide significant information on how different GC content in the mRNA sequence could affect ...
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Determining Physical Mechanisms of Gene Expression Regulation from Single Cell Gene Expression Data
... single cell gene expression ...a gene across two different cell populations, using a combination of the Bayesian Information Criterion (BIC), Marginal Probability (MP), and a ...
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Cell free expression and in meso crystallisation of an integral membrane kinase for structure determination
... out expression in the absence of a membrane mimetic, in part, because the aggregated protein so formed resembled the inclusion bodies that the protein, overexpressed in vivo, forms naturally and that has been used ...
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Regulation of sterol carrier protein 2 (SCP2) gene expression in rat peritoneal macrophages during foam cell formation A key role for free cholesterol content
... SCP2 gene expression in macrophages, while incubation with maleylated BSA had no ...SCP2 gene expression in macrophages. The enhancement of SCP2 gene expression by AcLDL suggests ...
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Single-Cell Gene Expression Variation as A Cell-Type Specific Trait: A Study of Mammalian Gene Expression Using Single-Cell RNA Sequencing
... of gene characteristics for use in bias ...minimum free energy predicted by Vienna RNAFold (version ...relative expression level of isoforms estimated by RSEM quantification of bulk HBR or ...the ...
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<p>Long noncoding RNA HCP5 suppresses skin cutaneous melanoma development by regulating <em>RARRES3</em> gene expression via sponging miR-12</p>
... by cell prolifera- tion, colony formation, apoptosis and transwell invasion ...Low expression of HCP5 was associated with SKCM patients ’ poor overall survival and disease ...HCP5-co-expressing gene ...
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Chronic arsenic trioxide exposure leads to enhanced aggressiveness via Met oncogene addiction in cancer cells
... of cell survival and ...parental cell line, = 1 - the drug is equally active in the resistant and parental cells and >1 - the given drug is more active in the parental cell line as compared to the ...
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Metabolic engineering to enhance biosynthesis of both docosahexaenoic acid and odd-chain fatty acids in Schizochytrium sp. S31
... ELO3 gene derived from Mortiere- lla alpina has been well characterized with a high activ- ity of converting C16 into C18 [33, ...ELO3 gene was chosen and trans- formed into Schizochytrium ...ELO3 ...
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Contextual tumor suppressor function of T cell death associated gene 8 (TDAG8) in hematological malignancies
... c-myc expression at physiological pH ...oncogene expression are partially due to TDAG8-medi- ated Gα13/Rho signaling ...cancer cell clonal evolution parallel to extracellular aci- dosis found within ...
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Identification of four hub genes as promising biomarkers to evaluate the prognosis of ovarian cancer in silico
... Weighted gene co-expression network analysis; OS: Overall survival; PFS: Progression-free survival; GEO: The Gene Expression Omnibus; DEGs: Different expression genes; TOM: ...
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AP Bio Ch 18 guided reading
... regulate cell growth, and should not be stuck “on,” much like the accelerator in a car; and tumor-suppressor genes, which work like the brakes on a car and must function! Let’s begin with a look at the ras ...
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Quantification of cell identity from single cell gene expression profiles
... each cell identity and each cell type is given the same level of diagnostic ...mean expression of all genes in the predefined marker set and weight each gene by its Spec score for the ...
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miR 24 2 controls H2AFX expression regardless of gene copy number alteration and induces apoptosis by targeting antiapoptotic gene BCL 2: a potential for therapeutic intervention
... H2AX gene is not essential, but its absence shows increased genomic instability and sensi- tivity to DNA damaging agents ...H2AX gene expression ...in cell proliferation and apoptosis ...and ...
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Profiling of transcriptional and epigenetic changes during directed endothelial differentiation of human embryonic stem cells identifies FOXA2 as a marker of early mesoderm commitment
... Data were quantile normalised and background subtracted in Genome Studio (Illumina®) and were then exported to Partek® Genomics Suite™ (Partek® Inc., St. Louis, MO, USA) and visualised using principle compo- nent ...
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