Chromatin immunoprecipitation
Chromatin immunoprecipitation assay detects er recruitment to gene specific promoters in uterus
... analyze chromatin DNA, its size specific fragmentation (usually within ≈ 500 bp) is necessary prior to chromatin immunoprecipitation in order to avoid amplification of unwanted DNA (20), and this can ...
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A fast, efficient chromatin immunoprecipitation method for studying protein-DNA binding in Arabidopsis mesophyll protoplasts
... Chromatin immunoprecipitation (ChIP) is a powerful tool for the investigation of interactions between DNA- binding proteins and genomic DNA in vivo ...
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Profiling gene promoter occupancy of Sox2 in two phenotypically distinct breast cancer cell subsets using chromatin immunoprecipitation and genome wide promoter microarrays
... ChIP (chromatin immunoprecipitation)-chip and ChIP-PCR ChIP-chip was performed based on a previously de- scribed ChIP-PCR protocol [18]. The starting material was scaled up four times, such that starting ...
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Protocol: Chromatin immunoprecipitation (ChIP) methodology to investigate histone modifications in two model diatom species
... a chromatin immunoprecipitation (ChIP) protocol for two fully sequenced model diatom species Phaeodactylum tricornutum and Thalassiosira ...of chromatin and gives reproducible ...
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Genome Wide Chromatin Immunoprecipitation Sequencing Analysis Shows that WhiB Is a Transcription Factor That Cocontrols Its Regulon with WhiA To Initiate Developmental Cell Division in Streptomyces
... WhiB is the founding member of a class of actinomycete- specific proteins known as the Wbl (WhiB-like) family (14–16). Wbl proteins have been shown to contain four conserved cys- teines that form an oxygen- and nitric ...
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Protocol: fine-tuning of a Chromatin Immunoprecipitation (ChIP) protocol in tomato
... is chromatin immunoprecipitation, which was developed for Arabidopsis and other plants but is not yet operational for tomato (Solanum lycopersicum), a model plant species for a group of economically ...
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Protocol: methodology for chromatin immunoprecipitation (ChIP) in Chlamydomonas reinhardtii
... of chromatin structure is chromatin immunoprecipitation (ChIP), a method that was first introduced for Droso- phila [17] and has since then enormously gained in ...the chromatin state by ...
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Inference of RNA Polymerase II Transcription Dynamics from Chromatin Immunoprecipitation Time Course Data
... In addition to estimating the transcriptional activity at the promoter, inferring the pol-II occupancy time profiles over different gene segments allows us to compute the transcription s[r] ...
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Chromatin immunoprecipitation: optimization, quantitative analysis and data normalization
... In this paper we provide a robust, optimized ChIP proto- col, useful handles for setting up this technique, and strat- egies on how to further optimize the technique for different plant species and tissues of interest. ...
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Inhibition of p300 lysine acetyltransferase activity by luteolin reduces tumor growth in head and neck squamous cell carcinoma (HNSCC) xenograft mouse model
... inhibition of cancer progression. In order to understand the direct effect of luteolin mediated inhibition of histone acetylation on gene expression, we performed a chromatin immunoprecipitation analysis ...
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Genetic variants in regulatory regions of microRNAs are associated with lung cancer risk
... In silico analysis In order to evaluate whether promising SNPs harbor regulatory elements, we downloaded Chromatin Immunoprecipitation sequencing ChIP-seq and DNaseI Hypersensitivity DNa[r] ...
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A Chromatin Insulator-Like Element in the Herpes Simplex Virus Type 1 Latency-Associated Transcript Region Binds CCCTC-Binding Factor and Displays Enhancer-Blocking and Silencing Activities
... repressed chromatin domains, respec- ...that chromatin insulators, similar to those of cellular chromosomes, may separate distinct transcriptional domains of the HSV-1 latent ...distinct chromatin ...
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Distinctive patterns of epigenetic marks are associated with promoter regions of mouse LINE-1 and LTR retrotransposons
... into chromatin associated with repressive histone ...These chromatin modifications are functionally interrelated and coordinate epigenetic silencing of ...the chromatin states responsible for ...
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PARI functions as a new transcriptional target of FOXM1 involved in gastric cancer development
... and chromatin immunoprecipitation assay further demonstrated that FOXM1 directly regulated PARI transcription by binding to the specific sequences of PARI ...
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DNA methylation and epigenomics: new technologies and emerging concepts
... low-input chromatin immunoprecipitation sequencing (ChIP-seq) method, Matthew Lorincz (University of British Columbia, Canada) reported that class I and class II long terminal repeat (LTR) retrotransposons ...
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Zinc Finger and X-Linked Factor (ZFX) Binds to Human SET Transcript 2 Promoter and Transactivates SET Expression
... Abstract: SET protein carries out multiple functions including those for protein phosphatase 2A (PP2A) inhibition, histone modification, DNA repair and gene regulation. SET overexpression has been detected in brain ...
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Characterization of Transcription Factor Binding to Human Papillomavirus Type 16 DNA during Cellular Differentiation
... by chromatin immunoprecipitation assays, we have shown that many of the transcription factors identified in the above screen bind to the HPV-16 promoter/ regulatory sequences in vivo and that the level of ...
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An enChIP system for the analysis of bacterial genome functions
... cule-mediated chromatin immunoprecipitation (enChIP) technology we developed recently, enables specific isola- tion of genomic regions of interest interacting with their associated molecules [1, ...
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Kaposi's Sarcoma-Associated Herpesvirus-Encoded LANA Interacts with Host KAP1 To Facilitate Establishment of Viral Latency
... by chromatin immunoprecipitation for sequencing (ChIP-seq) and demonstrated that LANA-recruited KAP1 played a critical role in the shutdown of lytic gene expression during the early stage of KSHV primary ...
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Tip60 complex binds to active Pol II promoters and a subset of enhancers and co-regulates the c-Myc network in mouse embryonic stem cells
... by chromatin immunoprecipitation (ChIP) coupled with high-throughput sequencing (ChIP-seq) shows that the Tip60 complex is present at promoter regions of predominantly active genes that are bound by RNA ...
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