coli single-stranded DNA binding protein
Thermodynamic analysis of DNA binding by a Bacillus single stranded DNA binding protein.
... 70 binding suggested that SSB BA forms a tetrameric assembly upon ssDNA ...for DNA binding through the formation of a transient tetrameric ...the protein-DNA complex required in ...
17
Displacement of the canonical single stranded DNA binding protein in the Thermoproteales
... Abstract Single-stranded DNA binding proteins (SSBs) based on the OB-fold are considered ubiquitous in nature and play a central role in many DNA transactions including replication, ...
30
Nanopore Analysis of Single-Stranded Binding Protein Interactions with DNA
... the binding of E. coli single-stranded binding protein (SSB) to single-stranded DNA (ssDNA) using a solid-state nanopore ...double-stranded DNA ...
17
High affinity RNA binding by a hyperthermophilic single stranded DNA binding protein
... replication protein A (RPA) in eukaryotes, play a central role in DNA replication, recombination, and ...bind DNA, and typically oligomer- ise in solution to bring multiple OB fold domains together ...
11
Dynamics of \u3cem\u3eE. Coli\u3c/em\u3e Single Stranded DNA Binding (SSB) Protein-DNA Complexes
... 35 binding mode [56] ...SSB binding mode has been observed with the RecQ ...complex DNA structures such as double-Holliday junctions, displacement loops (D-loops) and converging replication ...
23
A Single-stranded DNA-binding Protein of Bacteriophage T7 Defective in DNA Annealing
... of DNA is a vital step during the process of DNA replication, recom- bination, and ...a single-stranded DNA- binding protein, performs this ...a single amino acid ...
10
Binding of the pseudorabies virus immediate-early protein to single-stranded DNA.
... Results of early studies have shown that ICP4 from infected cell extracts binds to single-stranded DNA-cellulose columns (6).. Wilcox et al (17) have reported that ICP4 exists in several[r] ...
6
Phylogenetic and Functional Analysis of the Bacteriophage P1 Single-Stranded DNA-Binding Protein
... a single-stranded DNA-binding protein (SSB-P1), which shows 66% amino acid sequence identity to the SSB protein of the host bacterium Escherichia ...E. coli counterpart as ...
7
High-resolution AFM imaging of single-stranded DNA-binding (SSB) protein DNA complexes
... the binding at high monovalent salt ...trigger DNA adsorption, according to the diagram of dsDNA adsorption on mica in presence of spermidine ...ssDNA–E. coli SSB complexes, formed and adsorbed in a ...
8
Reovirus Protein ςNS Binds in Multiple Copies to Single-Stranded RNA and Shares Properties with Single-Stranded DNA Binding Proteins
... NS protein migrates as 7- to 9S complexes in 5 to 20% sucrose gradients (10), consistent with oligomers contain- ing three to six 41-kDa monomers of ...the binding unit, that is, if the concentrations of ...
10
Identification of a Region of the Herpes Simplex Virus Single-Stranded DNA-Binding Protein Involved in Cooperative Binding
... major DNA-binding protein (ICP8) which is involved in cooperative binding to single-stranded ...The binding properties of the modified protein were analyzed by ...
9
Characterization of the Single-Stranded DNA Binding Protein Encoded by the Vaccinia Virus I3 Gene
... during DNA replication and exhibits the strongest and most specific affinity for ...plasmid DNA and maintained at 37°C under G418 selection until 3 days ...
10
The baculovirus single-stranded DNA binding protein, LEF-3, forms a homotrimer in solution.
... transient DNA replication and has the properties of a single-stranded DNA binding ...transient DNA replication, suggesting that this interaction is required for the proper ...
6
Cloning and characterization of a single-stranded DNA binding protein that specifically recognizes deoxycytidine stretch
... A clone (SW2) of this series contained a near full length cDNA encoding a putative peptide with 463 amlno acid residues. We isolated this peptide as a fusion protein. It was found that t[r] ...
6
The major herpes simplex virus DNA-binding protein holds single-stranded DNA in an extended configuration.
... Herpes simplex virus proteins: DNA binding proteins in infected cells and in the virus structure. Nucleotide sequence of bacteriophage fd DNA[r] ...
6
Binding dynamics of single-stranded DNA binding proteins to fluctuating bubbles in breathing DNA
... We solve the general case numerically i.e., including SSB binding and the loop closure as a function of statistical weight u, binding protein size λ and binding strength κ, and compare t[r] ...
34
Developing novel single molecule analyses of the single stranded DNA binding protein from Sulfolobus solfataricus
... Alone, the PELDOR results did not point towards a strong protein protein interface. Even with the stabilising presence of ssDNA the results seemed to discount the formation of rigid filaments. This was ...
221
Structure-function studies of late stages of E. Coli MMR : interaction of DNA helicase II with single-stranded DNA binding protein (SSB) and MutL
... in DNA MMR. One model, the translocation model, proposes that the DNA-bound MutS moves along the helix and that this movement is coupled to adenosine triphosphate binding and hydrolysis (Blackwell ...
158
Mechanism of Replication of φX174 Single-Stranded DNA IX. Requirement for the Escherichia coli dnaG Protein
... that the dnaG gene product is required for the synthesis of both strands of OX.. RF during RF replication and of the complementary strand and viral progeny.[r] ...
6
Conserved region 3 of the adenovirus type 5 DNA-binding protein is important for interaction with single-stranded DNA.
... Immunoprecipitation of mutant DBP with monoclonal antibodies (Ab). COS-1 cells were transfected with mutant DBP gene-containing plasmids and labeled between 64 and 66 h postin- fection w[r] ...
9