Complementation assay
Common and Specific Properties of Herpesvirus UL34/UL31 Protein Family Members Revealed by Protein Complementation Assay
... protein complementation assay (PCA) using the TEM-1  -lactamase fragments fused to UL31 and UL34 protein homologues was used to study protein-protein interactions in ...
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Split luciferase complementation assay to detect regulated protein-protein interactions in rice protoplasts in a large-scale format
... luciferase complementation (SLC) assay is one of methods that detect regulated protein-protein in- teractions in situ (Ozawa et ...the assay, N- and C-terminal frag- ments of luciferase are ...
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Detection and Characterization of Influenza A Virus PA-PB2 Interaction through a Bimolecular Fluorescence Complementation Assay
... The N-terminal domain of PA is responsible for binding of PA to PB2. To investigate the specificity of the interaction and map the domain of the PA protein that mediates the PA-PB2 interaction, we generated two groups of ...
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Functional Homologies between Avian and Human Alphaherpesvirus VP22 Proteins in Cell-to-Cell Spreading as Revealed by a New cis-Complementation Assay
... Efficient cis complementation of MDV cell-to-cell spreading with UL49 genes from other mardiviruses. The homologies (percent amino acid identities) between MDV VP22 and its orthologs from the two other Mardivirus ...
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Visualization of protein interactions in living Drosophila embryos by the bimolecular fluorescence complementation assay
... partners which means that only a subset of fusion pro- teins will associate with each other in living cells [38]. Moreover, in fluorescence complementation assays, fluorophore maturation causes a delay between ...
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Cysteine mutants of herpes simplex virus type 1 glycoprotein D exhibit temperature-sensitive properties in structure and function.
... When we analyzed each of the mutants in a complementation assay for virus function 38, only two mutant proteins, Cys-2 and Cys-4, Glycoprotein D gD of herpes simplex virus HSV is a struc[r] ...
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A Domain in the Herpes Simplex Virus 1 Triplex Protein VP23 Is Essential for Closure of Capsid Shells into Icosahedral Structures
... genetic complementation data, except for L108A and I219A, which gave higher yields in the single-step growth assay than the complementation ...
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Dissecting the Role of the N-Terminal Domain of Human Immunodeficiency Virus Integrase bytrans-Complementation Analysis
... cross- complementation experiments were done by using FIV and HIV-2 IN ...the complementation assay may be different from that of chimeric proteins and can therefore affect the ...the ...
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Subcellular localization of the Vif protein of human immunodeficiency virus type 1.
... To determine whether regions important for the association of Vif with membranes are also important for function, a transient complementation assay was used to examine the abilities of t[r] ...
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ISOLATION AND GENETIC ANALYSIS OF MUTATIONS AT THE SerH IMMOBILIZATION ANTIGEN LOCUS OF TETRAHYMENA THERMOPHILA
... A diffus- ible trans-acting regulator substance might be expected to produce a positive result on the in vivo complementation assay where it would have approximately 4[r] ...
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Argument Frame Extraction and Term Clustering from an English French Bilingual Aligned Corpus
... Thus the rst group is those patterns consisting of the verb in question, a sequence of zero or more optional items, and a word tagged RP (i.e. preposi- tional adverb or particle, such as about or in ). This was included ...
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Pseuduo-Complementation on Almost Lattices
... pseudo- complementation on an AL with 0, then we proved that each maximal element of gives rise to a pseudo-complementation and this correspondence is ...pseudo- complementation ∗ ...
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RECOMBINATION AND COMPLEMENTATION OF ALBINO MUTANTS IN NEUROSPORA
... Moreover, while the albino mutants contain only the colorless polyenes, complementing pairs pro- duce in their heterokaryons a full spectrum of colorless and pigmented carotenoids th[r] ...
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The Cloning by Complementation of the pawn-A Gene in Paramecium
... Figure 7.—The sequence and translation of the putative ORF of the pawn-A gene (GenBank accession no. AF050753). The intron sequence is presented along with the location of several oligonucleotides used to generate cDNA. ...
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An F1 Genetic Screen for Maternal-Effect Mutations Affecting Embryonic Pattern Formation in Drosophila melanogaster
... 86 complementation groups with two alleles on ...45 complementation groups represent most previously known maternal genes, while 41 complementation groups represent new loci, including several ...
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Decomposing non-redundant sharing by complementation
... present, complementation has never been exploited from a practical point of ...that complementation requires the implementation of a full integration between components ...
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Interallelic Complementation at the Mouse Mitf Locus
... 1996) where it can activate expression from pigment Heteroallelic combinations of Mitf mutations: To per- cell, mast cell, and osteoclast specific promoters (Bent- form a systematic study of the Mitf-associated ...
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Complementation of defective reovirus by ts mutants.
... 0 the genome of defective virus had maintained cores obtained from "C-labeled R2 virus was added as its integrity during passage with the R2E mu- a density marker; 0 3H-labeled cores obt[r] ...
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Expanding the repertoire of gene tools for precise manipulation of the Clostridium difficile genome: allelic exchange using pyrE alleles
... For many years the gene tools available to bring about specific, directed modifications of C. difficile at the genetic level were scant indeed. In the present study we have capitalised on the development of ACE ...
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