construction of expression vector
Construction, characterisation and utilisation of a novel E coli expression vector
... 1984). Expression was obtained in ...This construction in the host W5445 directed expression of CPG2 to between 1Z and ...of expression, compared to the native promoter ...The ...
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Construction of a recombinant eukaryotic expression vector containing <em>DNM3</em> gene and its expression in colon cancer cells
... tion. Vector PCDH-CMV-MCS-EF1a-GFP-Puro-DNM3 and plasmid PCDH-CMV-MCS-EF1a-GFP-Puro were transfected into SW620 and LoVo cells by Opti-MEM and Lipofectamine™ 2000 reagent according to the manufactur- er’s ...
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Expression Vector Construction and Genetic Transformation of Paeonia lactiflora Gibberellin 20 Oxidase Gene
... an expression vector in the present study and then transformed it into Arabidopsis thaliana plants by floral dip ...efficient vector tool and functional information of PlGA 20 ox for future gene ...
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Construction and transformation of expression vector containing Panax japonicus SS gene.
... By full-length and core conserved segment cloning of the SS gene, the plant expression vectors pCXSN-PjSS and pCXSN-antiPjSS were constructed and transformed into Agrobactria LBA4404. PCR showed that there was a ...
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Construction of a recombinant eukaryotic expression vector containing PHD3 gene and its expression in HepG2 cells
... PHD3 expression was significantly increased after therapy with epirubicin, alone or in combination with tamoxifen, in patients with T2-4 N0-1 breast cancer; however, PHD3 expression was not relevant in ...
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CONSTRUCTION OF YEAST EXPRESSION VECTOR CONTAINING ELT-B GENE FOR HIGH LEVEL OF HEAT LABILE ENTEROTOXIN-B SUBUNIT (LT-B)
... expressing vector for the LT-B protein, the elt-b gene encoding LT-B was amplified from an origin ...The expression vector was constructed by inserting the elt-B gene into the pYES shuttle ...
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Expression Vector Construction and Genetic Transformation of Rosa rugosa β l,3 Glucanase Gene (RrGlu)
... the expression of β-1,3-glucanase gene is pretty low in ...its expression activity is obviously enhanced [21] ...The expression level of RrGlu gene was extremely low in normal ...its ...
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Construction of Double Gene Expression Vector
... bacterial expression vector where the two genes can be controlled ...gene expression vector contains two independent transcriptional ...gene expression vector (pUB-S-X-T7) was ...
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Construction of Overexpression Vector of ACBP Gene in Zygosaccharomyces rouxii
... tion, which make it contains the same restriction enzyme site. And then let the gene fragment was connected with expression vector, recombination plasmid transformated state feelings E. coli . Bacterial ...
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Construction of interference vector targeting <em>Ep-CAM</em> gene and its effects on colorectal cancer cell proliferation
... a vector construction ...miRNAi Expression Vector Kit with EmGFP, the double stranded miRNA oligo was inserted into the miRNA expression vector of ...
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Antibiotic-free selection in E. coli: new considerations for optimal design and improved production
... an expression vector in a strain with a defect or inhibited expression of the same essential ...in construction of a dapD - mutant strain and dependence towards defined culture media ...
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Construction of a Shuttle Vector for Heterologous Gene Expression in Escherichia coli and Microalgae Anabaena
... gene expression in microalgae ...shuttle expression vector, a gfp gene from the pGFP plasmid was PCR amplified by using gfp primers (Table ...pAnFP vector to yield plasmid ...
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BmICE-2 interference vector construction and its silencing effect in the BmN-SWU1 cells
... The designed microRNA-siBmICE-2 and microRNA-siBmICE-2-control sequences were synthesized by Gene script (Nanjing, China). Synthesized microRNA-siBmICE-2, microRNA-siBmICE-2-control and PIZ/V5-dsRed plasmids were ...
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Support Vector Machines for Paraphrase Identification and Corpus Construction
... corpora construction in- volves mining paraphrase strings or sentences from news articles, with document clustering typically providing the topical coherence neces- sary to boost the likelihood that any two arbi- ...
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Rev dependent lentiviral expression vector
... Rev-dependent vector should maintain responsiveness to Tat expression as ...Rev-dependent vector in only one application (viral reporter vector), an application that is challenging and not ...
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p53 Protein Is a Suppressor of Papillomavirus DNA Amplificational Replication
... Bcl2 expression did not affect the suppression of BPV-1 origin replication in the same ...the expression of p53 or its ...the expression of p53 or its mutants, because those factors are presumably ...
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Article - Encoding of BT (Bacillus thuringiensis) pro tein by fu sion gene
... Preparation and Purification of toxins from E.coli cell : Freshly grown single colony of BL21 (DE3) containing cry1Ac-vip3A plasmid was inoculated into 100ml LB broth and incubated at 37 0 C in a water bath shaker with ...
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Vesicular stomatitis virus matrix protein inhibits host cell-directed transcription of target genes in vivo.
... To determine whether the viral matrix M protein was involved in this inhibition of host cell gene expression, an M protein expression vector was cotransfected with a target gene vector, [r] ...
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Hepatitis B Virus X Protein Induces Perinuclear Mitochondrial Clustering in Microtubule- and Dynein-Dependent Manners
... Analysis of mitochondrial clustering. Mitochondrial clustering was judged by two criteria. When the area occupied by mitochondria in a cell was less than 40%, crescent-shaped or ring-shaped mitochondrial staining was ...
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Immunoreceptor Tyrosine-Based Activation Motif-Dependent Signaling by Kaposi's Sarcoma-Associated Herpesvirus K1 Protein: Effects on Lytic Viral Replication
... an expression vector that uses the strong EF-1␣ promoter to direct expression of a K1 open reading frame tagged with a hemagglutinin epitope (HA-K1) was transfected into BJAB cells together with a ...
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