Construction of the expression vector
Expression Vector Construction and Genetic Transformation of Rosa rugosa β l,3 Glucanase Gene (RrGlu)
... 2.2.2. Construction of the Expression Vector PBI121-RrGlu The RrGlu fragment with Bamh I and Xba I ends was cloned into a PBI121 vec- tor. The obtained plasmid was transferred into E. coli DH5a. ...
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Construction and transformation of expression vector containing Panax japonicus SS gene.
... METHODS Construction of PjSS expression vector Cloned SS positive single colonies were selected and placed in 10 mL LB nutrient medium with the appropriate antibiotics ...
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Construction of Sly-miR393 Over-Expression Vector and Verification of Its Target Genes in Tomato
... 1.2.5 Construction of plant expression vectors Connect the PCR product recovered in the above step ...plant expression vector pLp100-35S ...pLP100-35S vector fragment 1 μl (50 ng/μl), ...
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Construction of a recombinant eukaryotic expression vector containing <em>DNM3</em> gene and its expression in colon cancer cells
... eukaryotic expression vector For the formation of the recombinant vector PCDH-CMV- MCS-EF1a-GFP-Puro-DNM3, the digested and purified PCR products and plasmids were ligated with DNA Ligation Kit ...
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Construction of Double Gene Expression Vector
... the expression of recombinant ...controlled expression of multiple genes. Controlled expression of multiple genes is a useful approach for various purposes: ...However, expression of some ...
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Construction, characterisation and utilisation of a novel E coli expression vector
... derived vector was the only orientation capable of producing a functional alpha-peptide, resulting in the pMTL26 ...derived vector created translational stop codons in the linker regions resulting in a ...
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Construction of a recombinant eukaryotic expression vector containing PHD3 gene and its expression in HepG2 cells
... PHD3 expression was significantly increased after therapy with epirubicin, alone or in combination with tamoxifen, in patients with T2-4 N0-1 breast cancer; however, PHD3 expression was not relevant in ...
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Construction of an Artificial MicroRNA Expression Vector for Simultaneous Inhibition of Multiple Genes in Mammalian Cells
... multi-amiRNA expression vectors. All amiRNA expression vectors constructed in this study could specifically inhibit the expression of corresponding target genes in mammalian ...multi-amiRNA ...
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Expression Vector Construction and Genetic Transformation of Paeonia lactiflora Gibberellin 20 Oxidase Gene
... overexpression vector of the PlGA 20 ox gene was success- fully constructed and the function was explored in this study, which will un- doubtedly facilitate the genetic transformation of herbaceous ...
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Construction of py-af Vector for Expression of Thermostable α-l-arabinofuranosidase in Saccharomyces cerevisiae
... The α- L -arabinofuranosidase from G. thermoleovorans IT-08 (abfA) has never been expressed in S. cerevisiae. We are interested to choose this enzyme because it has a thermostable characteristic that can be applied in ...
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Construction and Transformation of OsERA1 Gene Into Expression Vector and Response of Nipponbare-OsERA1 Transgenic Rice to Drought Stress
... into expression vector, introduce it into rice plant, and confirm the positive OsERA1-rice plants conferring drought ...an expression vector cassette, ...into expression vector ...
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CONSTRUCTION OF YEAST EXPRESSION VECTOR CONTAINING ELT-B GENE FOR HIGH LEVEL OF HEAT LABILE ENTEROTOXIN-B SUBUNIT (LT-B)
... expressing vector for the LT-B protein, the elt-b gene encoding LT-B was amplified from an origin ...The expression vector was constructed by inserting the elt-B gene into the pYES shuttle ...
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Construction of an Expression Vector Containing a Novel Fusion Sequence from Middle Region of NS3 and Truncated Core Genes of Hepatitis C Virus
... expressing vector and the ligation reaction drove them to join covalently together by exploiting T4 ligase and also pretreatment with alkaline ...This vector contains strong CMV enhancer-promoter to drive ...
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For the construction of the Egr1 expression plasmid, the pcmv-sport6-egr1 vector (Invitrogen),
... miR-199a-5p/-3p expression vector driven by mouse U6 (mU6) promoter and its functional analysis by reporter ...an expression plasmid vector for RNA containing the ...
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Construction of a Shuttle Vector for Heterologous Gene Expression in Escherichia coli and Microalgae Anabaena
... The construction of an integrative shuttle expression vector and potential utility was reported in Escherichia coli and Anabaena (Nostoc) ...The vector comprised of the following elements: (a) ...
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Recombinant Equine Arteritis Virus as an Expression Vector
... the construction of a replication-competent arterivirus expressing the GFP gene via an extra subgenomic ...virus vector appeared to be severely impaired compared to wild-type EAV, it directed the synthesis ...
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Construction of Overexpression Vector of ACBP Gene in Zygosaccharomyces rouxii
... express vector can be trans- formed exogenous gene and using G418 to positive screening in wild type ...the vector which overexpression of ...cerevisiae expression vector with kanMX gene to ...
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Construction of an Expression Plasmid (Vector) Encoding Brucella melitensis Outer Membrane Protein, a Candidate for DNA Vaccine
... 45 sec, 58 °C for 45 sec, and 72 °C for 45 sec. The PCR was continued for 30 cycles at 94 °C for 45 sec, 61 °C for 45 sec, and 72 °C for 45 sec. The final primer extension was performed at 72 °C for 10 min. PCR products ...
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Construction of an adenovirus type 7a E1A- vector.
... gene expression found in the ...transgene expression in the liver was maximally maintained 2 weeks postinfection, with a decline to baseline activity by the week 4 ...
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Vector algebra in the analysis of genome wide expression data
... This construction has analytic and pedagogic ...Constructing expression vectors Transcription-profiling experiments offer different kinds of measurements depending on the technology ...gene ...
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