definitive endoderm
Mouse ES and iPS cells can form similar definitive endoderm despite differences in imprinted genes
... The directed differentiation of iPS and ES cells into definitive endoderm (DE) would allow the derivation of otherwise inaccessible progenitors for endodermal tissues. However, a global comparison of the ...
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NGN3 Expression in Definitive Endoderm Progenitors Specifies Pancreatic Endocrine Precursors
... Xenopus endoderm induced the formation of endocrine pancreatic tissue but also led in the generation of exocrine fate ...lineage-restricted definitive endoderm progenitors (DEPs), that are receptive ...
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Characterization of mesendoderm: a diverging point of the definitive endoderm and mesoderm in embryonic stem cell differentiation culture
... represent definitive or visceral endoderm, owing to lack of molecular markers distinguishing two endodermal lineages (Grapin-Botton and Melton, 2000; Tam et ...anterior definitive endoderm is ...
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Stimulation of Activin A/Nodal signaling is insufficient to induce definitive endoderm formation of cord blood-derived unrestricted somatic stem cells
... developing endoderm gives rise to the lung, pan- creas, liver, thyroid and gastrointestinal ...normal endoderm specification during embryonic devel- opment [6,7] as well as in the in vitro differentiation ...
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Definitive endoderm differentiation is promoted in suspension cultured human iPS derived spheroids more than in adherent cells
... ABSTRACT Human pluripotent stem cells (hPSCs), such as embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), are very attractive cell sources for the treatment of diabetes mellitus, because numerous ...
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A Study of the Role of Gata6 in Definitive Endoderm Specification and Β-Cell Functionality by Genome Engineering of Pluripotent Stem Cells
... in definitive endoderm ...the endoderm stage, cell lines with mutations in one or both GATA6 alleles were differentiated into β-like ...in endoderm specification and β-like cell functionality ...
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Sequential allocation and global pattern of movement of the definitive endoderm in the mouse embryo during gastrulation
... the endoderm of mid-gastrula-stage embryos to different segments of the embryonic gut of early-somite-stage embryos was ...entire endoderm population of the MS embryo to achieve a comprehensive fate map of ...
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Application of three dimensional culture conditions to human embryonic stem cell derived definitive endoderm cells enhances hepatocyte differentiation and functionality
... Human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) provide an unlimited source for the generation of human hepatocytes, owing to their indefinite self-renewal and pluripotent properties. Both ...
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Single cell RNA seq reveals novel regulators of human embryonic stem cell differentiation to definitive endoderm
... Importantly, in the recovered cell order at the 36-h time point, CXCR4+ and SOX17+ single cells appear later and are almost mutually exclusive to the POU5F1high and Thigh cells, indicati[r] ...
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Defined three-dimensional culture conditions mediate efficient induction of definitive endoderm lineage from human umbilical cord Wharton’s jelly mesenchymal stem cells
... The endodermal potential of WJ-MSCs has not been well established to date because only limited information has been reported on their endodermal differentiation capacity. Bhandari et al. [7] reported successful ...
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Signaling pathways during maintenance and definitive endoderm differentiation of embryonic stem cells
... FGF signaling is crucial for embryonic development as revealed by peri-implantation lethality of several mutants and by the expres- sion of FGF4 in the epiblast (reviewed in (Lanner and Rossant, 2010)). FGFs are released ...
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Wnt6 activates endoderm in the sea urchin gene regulatory network
... the endoderm, or markers that are expressed only in endoderm at the stage of the assay ...the endoderm (see Fig. 3 below). Of the endoderm genes, FoxA and Hox11/13b are expressed first in the ...
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The Vg1 related protein Gdf3 acts in a Nodal signaling pathway in the pre gastrulation mouse embryo
... and definitive endoderm formation; however, as Nodal expression is perturbed in the absence of Gdf3, we are unable to distinguish whether the gastrulation phenotypes of Gdf3 mutants represent primary or ...
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The impact of hepatocyte nuclear factor-1α on liver malignancies and cell stemness with metabolic consequences
... visceral endoderm in the yolk sac expressed HNF1 at around ...hESC-derived definitive endoderm (DE) and revealed that the PI3K pathway regulates hepatocyte differentiation from DE by increasing HNF1 ...
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Chato, a KRAB zinc finger protein, regulates convergent extension in the mouse embryo
... the definitive endoderm could be the result of mediolateral cell ...the definitive endoderm using phospho-histone H3 antibodies ...the definitive endoderm contained 0-3 ...
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Transplantation of MHC-mismatched mouse embryonic stem cell-derived thymic epithelial progenitors and MHC-matched bone marrow prevents autoimmune diabetes
... Abs: Antibodies; BM: Bone marrow; BMT: BM transplantation; DE: Definitive endoderm; EAE: Experimental autoimmune encephalomyelitis; GVHD: Graft- versus-host disease; HLA: Human leukocyte[r] ...
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Deconstructing the genesis of animal form
... By labeling definitive endoderm cells around the developing anterior foregut known as the anterior intestinal portal or AIP with the lipophilic fluorescent dialkycarbocyanine dyes DiI or[r] ...
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New markers for tracking endoderm induction and hepatocyte differentiation from human pluripotent stem cells
... generate definitive endoderm (see ...the definitive endoderm was induced either as EBs (as described above) or as a monolayer (Ogawa et ...
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The homeobox gene Hex is required in definitive endodermal tissues for normal forebrain, liver and thyroid formation
... and definitive endoderm The anterior defects in the forebrain of Hex −/− mutants cannot be due to a requirement for Hex function in the anterior neural tissue since Hex is not expressed in the neurectoderm ...
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A statistical approach for identifying differential distributions in single cell RNA seq experiments
... Abbreviations BIC: Bayesian information criterion; DD: differential distribution; DE: Differential expression; DEC: Definitive endoderm cell; DP: Differential proportion; DM: Differentia[r] ...
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