DNA extraction
Improved method for genomic DNA extraction for Opuntia Mill. (Cactaceae)
... the DNA extraction process. More specifically, during the DNA extraction process mucilage appears as soon as the tissue is pulverized, which significantly hinders the efficiency of the ...
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Comparison of different conditions for DNA extraction in sputum - a pilot study
... challenges could be identified. First, sputum is a substrate with a complex and very difficult-to-manage matrix. The use of different techniques for DNA extraction from spu- tum might be limited by its ...
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Permanent magnet actuation for magnetic bead-based DNA extraction
... extract DNA have been extensively developed using magnetic ...for DNA extraction, and compares the efficiency to the already available magnetic bead‑based DNA extraction ...
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Protocol: A high-throughput DNA extraction system suitable for conifers
... high-throughput DNA extraction protocol for conifers using an automated liquid handler and modifying the Qiagen MagAttract Plant Kit ...higher DNA yields per sample ...for DNA ...
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A high throughput DNA extraction method with high yield and quality
... chloroform extraction step was added after CTAB ex- traction buffer (similar to lysis ...chloroform extraction significantly improved the DNA yield and ...μl extraction buffer added to ...
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The effect of DNA extraction methodology on gut microbiota research applications
... of DNA extraction method on double stranded DNA yield, nucleic acids quality metrics and quantitative PCR ...stranded DNA concentration (bottom left) for the probiotic capsule, the absorbance ...
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Modified DNA Extraction for Rapid PCR Detection of Methicillin-Resistant Staphylococci
... PCR is a sensitive, specific and rapid method for the detection of mecA positive strains. Nevertheless, staphylococci cell walls are surrounded by the thick peptidoglycan [22]. To weaken staphylococcal cell wall in ...
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Optimized microbial DNA extraction from diarrheic stools
... prevent DNA hydrolysis on human non-diarrheic fecal samples ...in DNA recovery com- pared to fresh stool specimens ...to DNA extraction increased the sensitivity of real-time PCR-based ...
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Optimization of DNA extraction for advancing coral microbiota investigations
... DNA extraction from coral biomass for investigation of associated bacteria and archaea is particularly subject to technical challenges and potential ...during extraction may decrease PCR efficiency ...
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An Efficient Method for DNA Extraction from Preserved Stoneflies (Insecta, Plecoptera)
... on DNA amplification used both field and museum alcohol-preserved ...different DNA extraction protocols based on preserved stoneflies specimens with SDS-Ethanol method, Phenol/Cloroform, ...
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Comparison of three genomic DNA extraction methods to obtain high DNA quality from maize
... genomic DNA from the grains of cereal crop, Zea mays, with minor ...The DNA from the grains of two maize hybrids, M10 and M321, was extracted using extraction methods DNeasy Qiagen Plant Mini Kit, ...
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A New and Efficient Method for DNA Extraction from Human Skeletal Remains Usable in DNA Typing
... for DNA extraction from bone. Important factors that influenced DNA identification were: time duration since death, envi- ronmental effects, chemical factors that permeate inside bone tissue, ...
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Comparison of techniques for DNA extraction and agarose gel staining of DNA fragments using samples of Cryptosporidium
... the DNA extraction methods (I and II) used in this study were successful in breaking down the oocyst cell walls and collecting the DNA of the Cryptosporidium ...extracted DNA was evaluated in ...
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Comparison of Six DNA Extraction Methods for Recovery of Fungal DNA as Assessed by Quantitative PCR
... Aspergillus DNA detected in BAL fluid samples experimentally inoculated with ...different DNA extraction ...in extraction methods for ...lus DNA ( ⬎ 2,000 pg/ml) and were not ...
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Meischer s Discovery A DNA Extraction Laboratory
... the DNA is broken or sheared in too many places, it won’t spool and is harder to capture ...of DNA extraction and to avoid violent shaking or mixing that would shear the ...isolating DNA ...
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DNA Extraction from Insects by Using Different Techniques: A Review
... On the other hand, some insect species studied in relation to transmission of dengue virus, like Ades aegypti and A. albopictus. To conduct research on them, their larval colonies established which are time consuming, ...
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Protocol adaptation for brazil nut (Bertholletia excelsa Bonpl ) DNA extraction
... robust DNA extraction protocols to be applied to studies involving a large number of ...Some DNA extraction process stages are important to assure material in sufficient amount and quality, ...
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Soybean Seeds Sampling and DNA Extraction. Report on the Validation of a DNA Extraction Method from Soybean Seeds
... [DNA extraction, qualitative and quantitative PCR in the area of Biology (DNA extraction and PCR method validation for the detection and identification of GMOs in food and feed materials)] ...
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Evaluation of Two DNA Extraction Methods for Detection of Strongyloides stercoralis Infection
... for DNA extraction. One DNA extraction (QIA method) was performed using the QIAamp DNA stool minikit (Qiagen; Hilden, Germany) by following the manufac- turer’s protocol, with minor ...
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Validating DNA Extraction Protocols for Bentonite Clay
... template (up to 10 ng). Each PCR was prepared in triplicates, with two rounds by adding 1 l product from PCR1 into PCR2. The PCRs were performed as follows: 95°C for 3 min, 35 (PCR1) or 15 (PCR2) cycles of 95°C for 30 ...
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