DNA Extraction and PCR
Evaluation of DNA Extraction and PCR Methods for Detection of Enterocytozoon bienuesi in Stool Specimens
... The DNA pellet was resuspended in 200 l of TE buffer and kept at ⫺20°C until ...three DNA extraction methods, a stool specimen with a known concentration of microsporidial spores was ...extracted ...
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Rapid DNA extraction and PCR validation for direct detection of Listeria monocytogenes in raw milk
... the PCR technique used in this study was highly reproducible and statistically reliable, showing a kappa of ...Bansal PCR (17) and Makino DNA extraction (21) procedures, adapted to our ...
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Methods for microbial DNA extraction from soil for PCR amplification
... of DNA-based techniques can overcome this limitation by allowing the fate of particular genes or organisms to be monitored directly in environmental ...extract DNA from soil and sediment initially used ...
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Comparison of Six DNA Extraction Methods for Recovery of Fungal DNA as Assessed by Quantitative PCR
... gene PCR with analysis of cloned products to identify the contaminating species ...extracted DNA was subjected to qPCR in duplicate, and extractions were performed in duplicate or ...fungal DNA ...
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Modified DNA Extraction for Rapid PCR Detection of Methicillin-Resistant Staphylococci
... normal PCR. DNA extraction for PCR performance was then modified by omission of achromopeptadiase and proteinase K digestion, phenol/chloroform extraction and ethanol ...positive ...
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Optimisation of DNA Extraction from Individual Sand Flies for PCR Amplification
... fly; DNA extraction; Calcium; PCR, lysis buffer, Lutzomyia ...the extraction of DNA from phlebotomines, including methods that eliminate DNA-associated proteins by using ...
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quantitative real-time PCR, grain, simplex DNA extraction: PGS0426 RT-PCR: PGS0494 & PGS0476
... ® PCR procedure for the determination of the presence of sequence-containing DNA in Rice species DNA extracted from Rice grain ...single DNA extraction is performed followed by two ...
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Rapid Diagnosis of Extrapulmonary Tuberculosis by PCR: Impact of Sample Preparation and DNA Extraction
... complete DNA purification (cetyltrimethylammonium bromide [CTAB]-Roche), was conducted on 144 extrapulmonary specimens collected from 120 patients to evaluate the impact on the Cobas- Amplicor ...by PCR ...
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Protocol optimization for genomic DNA extraction and RAPD-PCR in mosquito larvae (Diptera: Culicidae)
... ABSTRACT PCR based molecular markers are powerful tools for the analysis of genetic diversity for which isolation of good quality genomic DNA is ...three DNA extraction methods for 1, 5 and 10 ...
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Simple and quick method of DNA extraction from different parts of plant for PCR amplification
... the DNA molecular marker based genetic ...genomic DNA is the prerequisite for molecular biology research. An efficient DNA extraction method should be simple, rapid and affordable to provide ...
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A rapid and efficient method of fungal genomic DNA extraction, suitable for PCR based molecular methods
... genomic DNA extraction from filamentous fungi with high-throughput ...alcohol extraction and precipitation with ...the DNA. Being a closed system of gDNA extraction, our method has been ...
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Evaluation of two DNA extraction methods for the PCR based detection of eukaryotic enteric pathogens in fecal samples
... easy-to-use DNA extraction and purification methods are critical in implementing PCR- based diagnosis of ...quantitative PCR cycle threshold (Ct) values, the efficiency of two DNA ...
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Comparison of DNA-extraction Methods Suitable for PCR-based Applications to Identify Shrimp Species in Commercial Products
... of DNA extraction were performed and compared in order to have the better DNA quality and quantity from specific seafood matrix such as various types of shrimp ...acids extraction efficiency ...
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Real-time PCR methods for detecting Salmonella spp. in food after different DNA extraction procedures
... real-time PCR (qPCR) protocols for the detection of Salmonella ...after DNA extraction using the InstaTM Gene matrix (BioRad, USA) and DNA extraction based on thermal cell ...applied ...
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Multicenter Comparison Trial of DNA Extraction Methods and PCR Assays for Detection of Chlamydia pneumoniae in Endarterectomy Specimens
... of standardization of diagnostic methods also may be respon- sible for false-positive as well as false-negative results. Our study was conducted to compare DNA extraction meth- ods and PCR ...
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Grapevine yellows: Comparison of different procedures for DNA extraction and amplification with PCR for routine diagnosis of phytoplasmas in grapevine
... plant DNA extraction and 4 procedures for amplifica- tion in polymerase chain reaction of phytoplasma DNA were examined in parallel using the same plant tissues infected with ...The DNA ...
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Evaluation of six different DNA extraction methods for detection of Mycobacterium tuberculosis by means of PCR IS6110: preliminary study
... The DNA extraction method influences the amplification efficiency, causing interference on the sensitivity and respective ...fast DNA extraction method, providing DNA amplification by ...
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Pathogen quantitation in complex matrices : a multi operator comparison of DNA extraction methods with a novel assessment of PCR inhibition
... Article Title: Pathogen Quantitation in Complex Matrices: A Multi- Operator Comparison of DNA Extraction Methods with a Novel Assessment of PCR Inhibition1. Year of publication: 2011 [r] ...
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Automated and Manual Methods of DNA Extraction for Aspergillus fumigatus and Rhizopus oryzae Analyzed by Quantitative Real Time PCR
... as PCR. One parameter, which influences the clinical usefulness of PCR, is the ability to effi- ciently isolate DNA from a clinical ...different DNA extraction methods that can be used ...
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Comparison of DNA extraction kits for PCR-DGGE analysis of human intestinal microbial communities from fecal specimens
... that PCR amplicons from the same set of samples be separated using DGGE gels of multiple gradients in order to better distinguish common and less common ...different DNA extraction ...since ...
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