Gene Expression by qRT PCR
Validation of reference genes for normalization of gene expression by qRT-PCR in a resveratrol-producing entophytic fungus (Alternaria sp. MG1)
... microorganisms. qRT‑PCR is an efficient and widely used method to identify the key genes related to unknown pathways at the level of gene ...for qRT‑PCR data normalization, although ...
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Protocol for qRT-PCR analysis from formalin fixed paraffin embedded tissue sections from diffuse large b-cell lymphoma: Validation of the six-gene predictor score
... normalized gene-expression values were log-transformed (on a base of 2) and the mortality predictor score was calculated based on the following equation: Mortality Predictor Score = ...
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Selection and evaluation of reference genes for qRT-PCR analysis in Euscaphis konishii Hayata based on transcriptome data
... reference gene in non-model plants can cause ...evaluate gene expression stabil- ity for qRT-PCR normalization ...Reference gene validation has been done in many plant species, ...
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Original Article Validation of reference genes for the normalization of qRT-PCR expression studies in head and neck squamous cell carcinoma cell lines treated by different chemotherapy drugs
... target gene expression profiling in head and neck squamous cell carcinoma ...the qRT-PCR method for analysis of gene expression were investigated via geNorm, NormFinder, and ...
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High purity high yield tandem B and T helper cell isolation for qRT PCR analysis suitable for basically equipped laboratories
... and gene expression was analysed by qRT-PCR ...AID expression in B cells isolated from a small amount of blood ...their expression levels in Th cells of malaria patients were ...
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Selection of reference genes for qRT PCR examination of wild populations of Atlantic cod Gadus morhua
... trustworthy gene expression data using real-time RT-PCR it is necessary to normalize the data with internal control genes, based on their mRNA ...their expression stability in order to normal- ...
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Evaluation of the effects of tropical tanniferous plants on rumen microbiota using qRT PCR and DGGE analysis
... Concerning methanogenic Archaea, different re- sults among legumes were observed at t ½ (Figure 1C). LEU and STA resulted in the highest decreases (0.58 and 0.29, respectively) in the relative gene ...
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Myostatin and IGF I signaling in end stage human heart failure: a qRT PCR study
... of gene expression or post- ...Mstn expression [32], and to be upregulated in various forms of cardiomyopathy [33,34] and myocardial ischemia ...the expression of Mstn during heart ...miR-208b ...
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Single-Cell Gene-Expression Analysis by Quantitative RT-PCR
... Many early trials focused on establishing a safe cell concentration range for mRNA recovery in the absence of ribonuclease inhibitors. Experiments using homogenized SCID.adh lysates gave an early indication that dilution ...
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<p>Identification of a transcription factor-microRNA network in esophageal adenocarcinoma through bioinformatics analysis and validation through qRT-PCR</p>
... Recently, bioinformatics analysis has gradually been uti- lized to explore the mechanisms of cancer initiation and progression to identify important cancer-related genes, non- coding RNAs and TFs for further experimental ...
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Identification of novel biomarkers affecting the metastasis of colorectal cancer through bioinformatics analysis and validation through qRT-PCR
... regulate gene expression either in cis or in trans by recruiting chromatin-modifying com- plexes to promoters of target genes [7, ...Weighted gene co-expression network analysis (WGCNA) has ...
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Identification of differentially expressed proteins of Arthrospira (Spirulina) plantensis-YZ under salt-stress conditions by proteomics and qRT-PCR analysis
... same gene has different ex- pression products, (2) the same protein can form differ- ent spots due to different structural modification, and (3) the same protein can produce multiple protein frag- ments due to ...
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Limited Dynamic Range of Immune Response Gene Expression Observed in Healthy Blood Donors Using RT-PCR
... the expression of a set of genes involved in immune re- sponses. QRT-PCR was used to measure immune-related gene expression in whole blood samples, using procedures designed to sustain ...
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Original Article Upregulation of HOXA13 as a potential tumorigenesis and progression promoter of LUSC based on qRT-PCR and bioinformatics
... Enrichment analysis: Five hundred six co- expression genes of HOXA13 were annotated in GO. There were three categories of GO, including biological process (BP), molecular function (MF), and cellular component ...
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Evaluating L1CAM expression in human endometrial cancer using qRT-PCR
... total gene specificity of the nucleotide sequences chosen for the primers and ...A-3’-TAMRA. PCR reactions were performed as recently described ...miRNA expression was normalized to RNU6B (Assay ID ...
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<p>Downregulation of CDH16 in Papillary Thyroid Cancer and Its Potential Molecular Mechanism Analysed by qRT-PCR, TCGA and in silico Analysis</p>
... CDH gene family and is localized to chromosome 16, might be involved in some certain ...CDH16 expression in PTC are required to verify this ...CDH16 expression in PTC, deter- mine the regulatory ...
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Gene expression study using real time PCR identifies an NTR gene as a major marker of resistance to benznidazole in Trypanosoma cruzi
... down-regulated expression was found for both NTR and OYE genes, the number of gene copies was calculated using the qPCR ...NTR gene had two copies per diploid genome in the paren- tal strain and just ...
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The gene expression analysis of Arabidopsis thaliana ABC transporters by real-time PCR for screening monolignol-transporter candidates
... All gene expression data were analyzed, and a heat map was ...The expression patterns were roughly divided into two blocks, with one including clusters I, II, and III, and the other including ...
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PCR-MEDIATED CLONING A ND EXPRESSION OF THE GENE FOR THE B-SUBUNIT OF VIBRIO CHOLERAE TOXIN ISOLATED RECENTLY IN IRAN
... Chromosomal DNA was isolated from a recent local clinical isolate of Vibrio cholera 01 (El Tor biotype) by the mini prep method of Owen et al. 30 with minor modifications. Polymerase chain reaction (PCR) was ...
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Expression of Aflatoxin B1 Regulatory Gene in Environmental Aspergillus parasiticus Isolates Using Real-Time PCR
... Mycotoxigenic filamentous fungi have the ability to contaminate a wide range of food and animal feed with one or more Aflatoxin (1). Food and feeds, especially in warm climates, are susceptible to invasion by ...
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