high-resolution melting (HRM)
High Resolution Melting Curve Analysis for Rapid Detection of Pyrazinamide Resistance in Mycobacterium tuberculosis Clinical Isolates
... costly. High-resolution melting (HRM) curve analysis is a simple technique and has been widely used for many ...in melting point (T m ) compared to reference DNA; it does not require the use ...
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High resolution melting analysis for molecular detection of multidrug resistance tuberculosis in Peruvian isolates
... with high TB incidence such as Peru. In this study, we evaluated High Resolution Melting (HRM) assay in Peruvian isolates for the detection of mutations within rpoB , katG genes and promoter ...
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Rapid Detection of Rifampicin and Isoniazid Resistant Mycobacterium tuberculosis by High Resolution Melting Analysis
... a high-resolution melting (HRM) assay to scan for mutations in the rpoB, inhA, ahpC, and katG genes and/or promoter regions for the detection of rifampin and isoniazid resistance in Mycobacte- rium ...
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Detecting differential allelic expression using high-resolution melting curve analysis: application to the breast cancer susceptibility gene CHEK2
... a high-resolution melting (HRM) analysis approach to perform allele-specific expression measurement in CHEK2 ...a high- throughput HRM device, the LightScanner ® instrument that uses a ...
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Development of a Rapid High Throughput Method for High Resolution Melting Analysis for Routine Detection and Genotyping of Noroviruses
... rapid, high-throughput detection and genotyping method for noroviruses using real-time reverse transcription-PCR (RT-PCR) and high-resolution melting (HRM) analysis to create a difference ...
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HBB FSC 36-37 (-T) Gene Mutation Detection in Carriers of Thalassemia Minor Using High Resolution Melting Analysis
... plot. High-resolution melting analysis could correctly identify all carriers of FSC 36-37 (-T) from who did not have this ...with high sensitivity and specificity for the identification of FSC ...
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The Use of High Resolution Melting Analysis for Detection of the CAPN530 Polymorphism in Slovak Simmental Bulls
... The high-resolution melting analysis is a cost-effective and simple post-PCR technique that can be used for SNP ...the high resolution melting analysis for detection of SNP ...
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Amino acid permease 3 (aap3) coding sequence as a target for Leishmania identification and diagnosis of leishmaniases using high resolution melting analysis
... by high-resolution melting analysis (HRM), generates thermodynamic differences in the dissociation profile of amplicons resulting in specific signatures of polymorphisms due to small differences in ...
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Identification of Phytophthora species by a high resolution melting analysis: an innovative tool for rapid differentiation
... the high resolution melting (HRM) analysis of the Ypt1 gene non-coding regions was validated for ten Phytophthora species with a broad host range from forest trees to crop ...The melting curve ...
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Comparison of a High Resolution Melting Assay to Next Generation Sequencing for Analysis of HIV Diversity
... Next-generation sequencing (NGS) has recently been used for analysis of HIV diversity, but this method is labor-intensive, costly, and requires complex protocols for data analysis. We compared diversity measures obtained ...
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High resolution melting analysis of the 18S rRNA gene for the rapid diagnosis of bovine babesiosis
... The high-resolution melting (HRM) assay is a novel and powerful molecular method applied to mutation detection, genotype analysis and species identifica- tion ...temperature, melting curve and ...
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The application of high resolution melting in the analysis of simple sequence repeat and single nucleotide polymorphism markers in a pea (Pisum sativum L ) population
... the high resolution melting (HRM) method in the analysis of single nucleo- tide polymorphism (SNP) and simple sequence repeat (SSR) markers in pea (Pisum sativum ...The melting curve shape ...
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Characterization of Genetic Diversity of Bacillus anthracis in France by Using High Resolution Melting Assays and Multilocus Variable Number Tandem Repeat Analysis
... Using high-resolution melting (HRM) analysis, we developed a cost-effective method to genotype a set of 13 phylogenetically informative single-nucleotide polymorphisms (SNPs) within the genome of ...
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Original Article High resolution melting method to detect single nucleotide polymorphism of VKORC1 and CYP2C9
... speed, high sensitivity and low cost for genotyping is calling for clinical ...laboratories. High resolution method (HRM) meets these callings, but no study with large sample tested its performance ...
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Evaluation of High Resolution Melting analysis as an alternate tool to screen for risk alleles associated with small kidneys in Indian newborns
... cost. High Resolution Melting analysis is a well estab- lished, closed tube, rapid and high throughput ...DNA melting and annealing in the presence of saturating DNA binding ...the ...
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Molecular detection of rifampin, isoniazid, and ofloxacin resistance in Iranian isolates of <em>Mycobacterium tuberculosis</em> by high-resolution melting analysis
... and high-resolution melting (HRM) curve analysis for the determination of resistance to rifampin (RIF), isoniazid (INH), and ofloxacin (OFX) in MTB isolates and to investigate their ...
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Rapid analysis of heterogeneously methylated DNA using digital methylation-sensitive high resolution melting: application to the CDKN2B ( p15 ) gene
... Methylation-sensitive high resolution melting (MS-HRM) methodology is able to recognise heterogeneously methylated sequences by their characteristic melting ...
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Rapid Differentiation of Influenza A Virus Subtypes and Genetic Screening for Virus Variants by High Resolution Melting Analysis
... C) High-resolution melting and derivative plots were obtained by heteroduplex formation (HD) between the PCR products of the indicated influenza A virus subtype and the reference strain of H1N1 ...
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High-Resolution Melting Analysis as an Appropriate Method to Differentiate between Fasciola hepatica and F. gigantica
... Background: Fasciolosis is a shared disease between humans and livestock caused by hepatic trematodes; Fasciola hepatica and F. gigantica. Differentiate between the two species of this genus is essential. ...
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Evaluation of spa-typing of methicillin-resistant Staphylococcus aureus using high-resolution melting analysis
... The high-resolution melting of the amplicons was performed between 70–95°C with a stepwise increase of ...The melting temperatures (Tm) were determined by the negative derivative of decreased ...
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