high throughput sequencing
Identification of microRNAs in PCV2 subclinically infected pigs by high throughput sequencing
... pigs. High throughput sequencing determined differences in miRNA expression in MLN between infected and non-infected while, in tonsil, a very conserved pattern was ...a high throughput ...
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Genotype-Frequency Estimation from High-Throughput Sequencing Data
... improving high-throughput sequencing technologies provide unprecedented opportunities for carrying out population-genomic studies with various ...from sequencing errors and biparental ...
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A Multicenter Study To Evaluate the Performance of High-Throughput Sequencing for Virus Detection
... (1). High-throughput sequencing (HTS), also called next-generation sequencing or massively parallel sequencing, is recognized as a powerful new technology for the detection and ...
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Characterizing the human vaginal microbiome using high-throughput sequencing
... using high-throughput sequencing methodologies to describe the vaginal ...V6 sequencing in order to describe the vaginal profiles of HIV+ African women with and without BV (48, ...using ...
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High-throughput sequencing of cytosine methylation in plant DNA
... Despite the progress being made in analysis of high- throughput sequencing data and specifically that pertain- ing to the methylome, there exist some clear areas for improvement. The question of ...
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Validation of high throughput sequencing and microbial forensics applications
... High throughput sequencing (HTS) generates large amounts of high quality sequence data for microbial ...2) sequencing, and 3) data ...
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Analysis options for high throughput sequencing in miRNA expression profiling
... RNA sequencing run using three pipelines (Figure ...member sequencing pipeline A) of trimmed reads to human genomic sequences using bowtie2 resulted in ...and high-throughput sequencing ...
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Investigation of the Plasma Virome from Cases of Unexplained Febrile Illness in Tanzania from 2013 to 2014: a Comparative Analysis between Unbiased and VirCapSeq-VERT High-Throughput Sequencing Approaches
... ABSTRACT High-throughput sequencing can provide insights into epidemiology and medicine through comprehensive surveys of viral genetic sequences in environ- mental and clinical ...two ...
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Targeted high-throughput sequencing for genetic diagnostics of hemophagocytic lymphohistiocytosis
... a high- throughput sequencing approach for the molecular diagnosis of patients with suspected ...genome sequencing and immuno- logical ...in high-throughput sequencing, ...
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Accounting for Errors in Low Coverage High-Throughput Sequencing Data When Constructing Genetic Maps Using Biparental Outcrossed Populations
... Next-generation sequencing is an ef fi cient method that allows for substantially more markers than previous technologies, providing opportunities for building high-density genetic linkage maps, which ...
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Assessing Illumina technology for the high throughput sequencing of bacteriophage genomes
... the sequencing of phage isolates have previously been discussed in detail (Klumpp, Fouts & Sozhamannan, ...Previously sequencing of phage genomes had been problematic due to cloning phage DNA for Sanger ...
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Linkage Disequilibrium Estimation in Low Coverage High-Throughput Sequencing Data
... low sequencing depth is to set genotype calls with an associated read depth below some threshold value to ...given sequencing cost (Dodds et ...representation sequencing data, particularly if SNPs ...
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High-throughput sequencing of <em>16S rDNA</em> amplicons characterizes bacterial composition in cerebrospinal fluid samples from patients with purulent meningitis
... with high rates of morbidity and ...using high-throughput sequencing ...that high-throughput sequencing approach facilitates the characterization of the pathogens in ...
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Characterization of a new apple luteovirus identified by high throughput sequencing
... by high-throughput sequencing Total reads of 27,727,559 (CPAR), 28,817,295 (CPAS1) and 30,614,167 (CPAS2) were obtained after removing the failed ...
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Delineating the genetic heterogeneity of ALS using targeted high throughput sequencing
... genomic sequencing libraries and target ...genomic sequencing libraries and target ...next-generation sequencing and related quality control at ...next-generation sequencing and related ...
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Automated degenerate PCR primer design for high throughput sequencing improves efficiency of viral sequencing
... The high-throughput degenerate PCR primer design pipeline has been very successful in providing the basis for high-quality viral sequencing results and for minim- izing costs associated with ...
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High-Throughput Sequencing Reveals miRNAs Affecting Follicle Development in Chicken
... the sequencing procedure is as follows: small RNAs ranging from 18 to 35nt in length was purified from 15% polyacrylamide gels, then ligated to 5’ and 3’ ...and sequencing analysis using the Illumina’s 2000 ...
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Spontaneous Mutation Discovery via High-Throughput Sequencing of Pedigrees
... human sequencing is the Heliscope sequencer, sold by ...Heliscope sequencing error profile, and incorporating a naïve prior probability distribution on the distribution of variation throughout the human ...
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Telomere and Proximal Sequence Analysis Using High-Throughput Sequencing Reads
... Despite the crucial role telomeres play in genome stability and their contribution to cancer and disease, telomere length varies widely in the human population. qFISH measurements of individual telomeres have shown that ...
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High-throughput Sequencing Analysis of the Actinobacterial Apatial Diversity in Moonmilk Deposits
... the most diverse bacterial population, as reflected by the highest diversity indices (Table 1, Figure 100.. 1a).[r] ...
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