ITS1/5.8S/ITS2
Genetic Variation among Fragmented Populations of Atriplex halimus L Using Start Codon Targeted (SCoT) and ITS1 5 8S ITS2 Region Markers
... The present study used two forms of A. halimus shrubs; erect habit (A. halimus) and bushy habit shrub (A. schweinfurthii). The two morphotypes are used naturally isolated by a considerable distance from each other and ...
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Phylogeny and PCR identification of the human pathogenic fungus Penicillium marneffei
... FIG. 1. Phylogenetic relationship of P. marneffei to Penicillium, Talaromyces, and Eupenicillium species inferred from combined nucleotide sequence of the internal transcribed spacers and 5.8S rRNA gene ...
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Molecular and Pathogenic Study of Guignardia spp Isolates Associated to Different Hosts
... Although restricted to one genome regions, variation on DNA sequence of rDNA ITS1-5.8S-ITS2 were effi- cient in classify the isolates of this study in species and also to infer genetic diversity among the ...
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Multigene-Based Analyses of the Phylogenetic Evolution of Oligotrich Ciliates, with Consideration of the Internal Transcribed Spacer 2 Secondary Structure of Three Systematically Ambiguous Genera
... Conclusions from the multigene phylogenetic analysis. When the ITS1-5.8S-ITS2 region was first applied to the study of oligotrichs and choreotrichs, it was suggested that the ITS and 5.8S regions could ...
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Genotypic Study of Pneumocystis jirovecii in Human Immunodeficiency Virus Positive Patients in Thailand
... To examine the genetic relationship of all 23 types of P. jirovecii found in Thailand, inferred trees under molecular phylogenetic methods were constructed from 498 aligned nu- cleotide positions of ...
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Genetic Characterization of Fasciola Isolates from West Azerbaijan Province Iran Based on ITS1 and ITS2 Sequence of Ribosomal DNA
... as ITS1, 5.8s, ITS2, COI, and ND1 (2, 8, 28, 42, ...of ITS1 region revealed that both ...of ITS1, 5.8s, and ITS2) construct- ed in the current study showed 100% similari- ty with ...
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PCR Detection of Pseudoperonospora humuli and Podosphaera macularis in Humulus lupulus
... Hop downy mildew (Pseudoperonospora humuli) and hop powdery mildew (Podosphaera macularis) are the most important pathogens of hop (Humulus lupulus). The early detection and identification of these pathogens are often ...
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Genotyping of Pneumocystis jirovecii by Use of a New Simplified Nomenclature System Based on the Internal Transcribed Spacer Regions and 5 8S rRNA Gene of the rRNA Operon
... and ITS2 regions of the ...jirovecii ITS1 and ITS2 genotypes have been obtained mostly by sequencing the ITS1 and ITS2 regions separately (8–11) and, less frequently, by sequencing a ...
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Rapid Identification of Yeasts in Positive Blood Cultures by a Multiplex PCR Method
... Yeasts are emerging as important etiological agents of nosocomial bloodstream infections. A multiplex PCR method was developed to rapidly identify clinically important yeasts that cause fungemia. The method ampli- fied ...
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Multilocus DNA Sequence Comparisons Rapidly Identify Pathogenic Molds
... at ITS1 and ITS2 ...of ITS1, ITS2, and ...at ITS1, ITS2, and 28S and are 100% identical at ITS1 and ITS2 to the ...
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Update on Pneumocystis carinii f sp hominis Typing Based on Nucleotide Sequence Variations in Internal Transcribed Spacer Regions of rRNA Genes
... and ITS2 is located between the 5.8S and 26S rRNA genes. ITS1 was found to contain 157 bp, and ITS2 was deter- mined to be 177 bp ...the ITS1 region that were found to be useful for typing are ...
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DNA Microarray Based on Arrayed Primer Extension Technique for Identification of Pathogenic Fungi Responsible for Invasive and Superficial Mycoses
... available ITS1 and/or ITS2 sequences (for accession numbers, see Table ...carrying ITS1, the 5.8S rRNA gene, and ITS2 and varied in size from 375 bp (Candida pulcherrima) to 880 bp (Candida ...
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The genetic variation of Angiostrongylus cantonensis in the People’s Republic of China
... Results: We characterised the complete mitochondrial genes cox1 and nad1 of 130 specimens and obtained 357 nuclear sequences containing two complete ITS (ITS1 and ITS2) and 5.8S rRNA of the same samples. ...
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Detection and Identification of Fungal Pathogens by PCR and by ITS2 and 5 8S Ribosomal DNA Typing in Ocular Infections
... extraction procedures for yeast and bacteria), and the presence of PCR inhibitors in human specimens (45) are some of the difficulties with fungal detection in ocular samples. The ideal marker to detect a fungal ...
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Multiplex PCR Using Internal Transcribed Spacer 1 and 2 Regions for Rapid Detection and Identification of Yeast Strains
... The ITS1-ITS4 primer pair was used to amplify the intervening ...adjacent ITS1 and ITS2 regions, and the ITS3-ITS4 primer pair was used to amplify a large portion of the ...adjacent ITS2 ...
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Biogenesis and Function of Small Non-Coding RNAs Derived from Eukaryotic Ribosomal RNA
... phasi RNA (rRF) pathway 4 Piwi RNA (rRF) pathway 3 5’ 3’ 3’ 5’ rDNA rRNA rRNA cleavage rRFs 5’ 3’ 3’ 5’ 5’ 3’ 3’ 3’ 5’. 18S 28S 45S rRNA mmu-miR-712 5.8S ITS1 ITS2 TIMP3 mRNA induce endo[r] ...
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Heligmosomoides neopolygyrus Asakawa & Ohbayashi, 1986, a cryptic Asian nematode infecting the striped field mouse Apodemus agrarius in Central Europe
... Heligmosomoides collected from A. agrarius in Poland appeared at first sight to belong to H. polygyrus, the common species recorded from Apodemus throughout Europe. The worms were red, slender and strongly coiled, with ...
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First evidence of Besnoitia bennetti infection (Protozoa: Apicomplexa) in donkeys (Equus asinus) in Belgium
... DNA ITS1 sequences are only available for ...No ITS1 sequence variation was found between Belgian and USA samples with the same T insertion at position 148 (Add- itional file ...and ITS1 sequences ...
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Identification of Hippophae species (Shaji) through DNA barcodes
... Seventy-five samples (Table 1) representing seven spe- cies and seven subspecies were collected from the major distribution areas, including Sichuan, Qianghai, Tibet, Yunnan, Beijing, and Xinjiang (China), between May ...
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Multiplex Tandem PCR: a Novel Platform for Rapid Detection and Identification of Fungal Pathogens from Blood Culture Specimens
... Primer design. To ensure accuracy and reproducibility and to identify possible intraspecies variation, consensus sequences were generated from available GenBank sequences with BioEdit (18) and CLUSTAL_W ...
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