lineage tracing
Lineage tracing reveals the dynamic contribution of Hes1+ cells to the developing and adult pancreas
... Two lineage tracing approaches have been taken to address this issue: ‘pulse-chase’ labeling of mature islet cells, to detect changes in labeling frequency caused by differentiation of new - cells ...
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In silico lineage tracing through single cell transcriptomics identifies a neural stem cell population in planarians
... genetic lineage tracing experiments used in other model ...cellular lineage progression from a par- ental ASC to differentiated ...resulting lineage changes pieced together in a temporally ...
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Genetic lineage tracing defines distinct neurogenic and gliogenic stages of ventral telencephalic radial glial development
... in lineage tracing experiments as a lack of recombination could reflect weak promoter activ- ity rather than restricted progenitor ...comparing lineage tracing results in which the same ...
12
Evidence of a developmental origin for β cell heterogeneity using a dual lineage tracing technology
... dual lineage-tracing system for both Pdx1 lineage and Ptf1a lineage cells in the same ...PTF1a-cre lineage-tagging has been reported (Burlison et ...Pdx1 lineage, which suggests ...
9
Direct lineage tracing reveals Activin-a potential for improved pancreatic homing of bone marrow mesenchymal stem cells and efficient ß-cell regeneration in vivo
... To evaluate the BMSC potential for repair and restoring lost ß-cell mass, we adopted the STZ-induced diabetic model for partial ß-cell ablation and mild hyperglycemia. As per National Institutes of Health (NIH) and the ...
12
In vivo genetic cell lineage tracing reveals that oviductal secretory cells self renew and give rise to ciliated cells
... The epithelial lining of the fallopian tube is vital for fertility, providing nutrition to gametes and facilitating their transport. It is composed of two major cell types: secretory cells and ciliated cells. ...
11
Lineage tracing of neuromesodermal progenitors reveals novel Wnt dependent roles in trunk progenitor cell maintenance and differentiation
... To test whether NMPs were being lost by cell death, we examined the PS of control (n=3) and Wnt3a −/− (n=3) embryos for apoptotic cells using an antibody to cleaved caspase 3. It has been previously shown that apoptosis ...
11
The complexities and caveats of lineage tracing in the mammary gland
... with lineage tracing strategies pertinent to the mammary ...to lineage tracing in the mam- mary gland are discussed ...between lineage tracing data in the mammary gland biology ...
5
Using schema transformation pathways for data lineage tracing
... data lineage tracing in AutoMed — the former derives all of the source data that had some influence on the tracing data, while the latter derives the specific data in the sources from which the ...
14
Persistence of RNAi-Mediated Knockdown in Drosophila Complicates Mosaic Analysis Yet Enables Highly Sensitive Lineage Tracing
... As an outcome of our work describing RNAi persistence in vivo, we developed the i-TRACE system as a novel method to monitor dynamic gene expression from Gal4 reporter lines. The i-TRACE system fills an important gap in ...
21
Cell lineage tracing during Xenopus tail regeneration
... 2 For the late medial PSM grafts, by 3 days after amputation the blastema contained some undifferentiated mononuclear cells expressing GFP, and by 5 days the labelling of the regeneratin[r] ...
11
tcf21+ epicardial cells adopt non myocardial fates during zebrafish heart development and regeneration
... looping. Lineage tracing studies in chick have shown that epicardial and epicardial-derived cells (EPDCs) within the subepicardial environment can differentiate into several cell types during heart ...
8
Non beta cell progenitors in pregnant mice and the origin and functionality of beta cells after diabetic recovery in a c Myc ablation model
... lineage tracing system that the Ngn3 positive cells are islet progenitors in adult mice. In addition, Guz et al. (Guz et al., 2001) reported the occurrence of two presumptive intra-islet precursors in the ...
203
Mammary gland development: cell fate specification, stem cells and the microenvironment
... confetti lineage tracing (A ′ ) shows that a few such K5 + cells in the terminal end bud (inset) give rise to all the cells in mature ducts (Rios et ...K14-based lineage tracing (B ′ ) shows ...
15
Dynamic somite cell rearrangements lead to distinct waves of myotome growth
... combine lineage tracing and gene expression analysis to test whether these Pax3- and Pax7-expressing cells are myogenic precursors and to identify their origin in ...
5
Sox9 and programming of liver and pancreatic progenitors
... in ducts. A detailed comparison of CreER expression in the different Sox9-CreER lines would be required to assess such variation. A more probable explanation is that the cell behavior is unexpectedly altered by the ...
7
Final_Honors_Thesis___2_.pdf
... The lineage tracing analysis of the SHF confirms that the right ventricle and IVS are positive for the SHF fate marker, Mef2c, which is predominantly absent in the left ...
20
Zebrabow: multispectral cell labeling for cell tracing and lineage analysis in zebrafish
... Stably maintained and inherited colors allow lineage tracing and fate mapping. Characterization of color profile of cells at an early time point allows re-identification of the cells with the same color ...
12
Lost in transdifferentiation
... for lineage tracing, the neointimal cells were SM22-LacZ negative, suggesting that the BMC-derived SM α -actin–positive intimal cells were some cell type other than smooth muscle ...
10
Endothelial WT1 and the epicardium in cardiac development and disease
... through lineage tracing aims to identify all progeny of a single cell population, based on a common ...study lineage tracing in tissue development, homeostasis, and disease [1, ...For ...
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