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Nucleic acid quantification and quality determination

Quantification of nucleic acid quality in postmortem tissues from a cancer research autopsy program

Quantification of nucleic acid quality in postmortem tissues from a cancer research autopsy program

... the nucleic acid quality in 371 different frozen tissue samples collected from 80 patients who underwent a research autopsy, including eight normal tissue types, primary and metastatic ...RNA ...

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Comparative Evaluation of Three Nucleic Acid Based Assays for BK Virus Quantification

Comparative Evaluation of Three Nucleic Acid Based Assays for BK Virus Quantification

... BKV quantification were not influ- enced by the various BKV genotypes or subtypes, with mean dif- ferences remaining lower than ...BKV quantification assays present de- creased sensitivity for detection of ...

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Rapid fluorometric quantification of bacterial cells using Redsafe nucleic acid stain

Rapid fluorometric quantification of bacterial cells using Redsafe nucleic acid stain

... ing on plates with high numbers of CFUs is error prone since it requires a high level of attention and so, often only parts of a plate are analyzed to esti- mate the whole plate count. Therefore, rapid methods for ...

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Determination and Quantification of Ferulic acid in Ferula asafoetida by a Validated HPTLC Method

Determination and Quantification of Ferulic acid in Ferula asafoetida by a Validated HPTLC Method

... ferulic acid which is one of a phenolic acid as an ingredient with a potent pharmacological ...ferulic acid from Ferula ...acetate-formic acid in the ratio 60:40:10 as mobile phases were ...

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Nucleic Acid Electrophoresis Cell

Nucleic Acid Electrophoresis Cell

... GT nucleic acid electrophoresis ...or nucleic acid separations, a 19:1 acrylamide:bis solution is ...boric acid and EDTA) solution is the preferred electrophoresis ...the quality ...

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Peptide Nucleic Acid Microarrays

Peptide Nucleic Acid Microarrays

... Reproducibility and reliability of PNA microarrays depend strongly on the quality of the oligomers on the chip surface. Also, relatively large numbers of oligomers are required for microarrays. However, only a ...

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Use of DNA and Peptide Nucleic Acid Molecular Beacons for Detection and Quantification of rrna in Solution and in Whole Cells

Use of DNA and Peptide Nucleic Acid Molecular Beacons for Detection and Quantification of rrna in Solution and in Whole Cells

... peptide nucleic acid (PNA) molecular beacons were successfully used to detect rRNA in ...the quantification of rRNA: hybridization signals increased in a linear fashion with the 16S rRNA ...

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SAFA: Semi-automated footprinting analysis software for high-throughput quantification of nucleic acid footprinting experiments

SAFA: Semi-automated footprinting analysis software for high-throughput quantification of nucleic acid footprinting experiments

... of nucleic acid folding and ligand binding ...gel quantification while reducing the time to analyze a gel from several hours to 15 min or ...in nucleic acid laboratories that otherwise ...

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Algorithms for nucleic acid sequence design

Algorithms for nucleic acid sequence design

... thermodynamic nucleic acid design a high-quality, versatile, and low-cost technology that is easy to use and easily ...of nucleic acids that also achieves high-quality sequence for low ...

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Peptide-nucleic acid nanostructures for transfection

Peptide-nucleic acid nanostructures for transfection

... with nucleic acids and how they can be used to overcome the existing ...and quality control as well as the possibility of well-established production processes at low and large scale, which are all ...

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Polysaccharides as initiators of nucleic acid polymerization

Polysaccharides as initiators of nucleic acid polymerization

... The mentioned quality of PS makes it possible to recon- sider their potential role in molecular evolution of bio- polymers of living matter. In view of the above and tak- ing into account the ability of PS to ...

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Rapid Development of Nucleic Acid Diagnostics

Rapid Development of Nucleic Acid Diagnostics

... A fortunate side effect of the use of a Suffix-Tree algo- rithm to determine candidate uniqueness is that we are not limited to using only completed genomes or finished DNA sequence. Our method works well on draft ...

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Genotypic identification of mycobacteria by nucleic acid sequence determination: report of a 2 year experience in a clinical laboratory

Genotypic identification of mycobacteria by nucleic acid sequence determination: report of a 2 year experience in a clinical laboratory

... We previously characterized hypervariable regions within the 16S rRNA molecule that exhibit species-specific charac- teristics in mycobacteria and proposed that direct sequence determina[r] ...

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A Scoring Method for the Clustering of Nucleic Acid Sequences

A Scoring Method for the Clustering of Nucleic Acid Sequences

... 1. INTRODUCTION In computational biology, clustering goes beyond a mere statistical tool for information retrieval. In sequence clustering, it aims at revealing the genetic information of participating sequences. Cluster ...

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Molecular Beacons of Xeno-Nucleic Acid for Detecting Nucleic Acid

Molecular Beacons of Xeno-Nucleic Acid for Detecting Nucleic Acid

... [62] nucleic acid capture ...electrochemical nucleic acids probe made of PNA which exhibits high sensitivity and specificity when challenged with heterogeneous samples of ...

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On nucleic acid photochemistry

On nucleic acid photochemistry

... General features. Ultraviolet action spectra for the formation of thymine dimer in E. coli DNA are shown in figs. Qual- itatively, the spectra may be interpreted to mea[r] ...

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Microarrays of Binary Nucleic Acid Probes for Detecting Nucleic Acid Analytes (US)

Microarrays of Binary Nucleic Acid Probes for Detecting Nucleic Acid Analytes (US)

... activated only when the two strands bind to the target analyte reconstituting the catalytic core. The activated enzyme then cleaves the bound substrate generating a signal localized [r] ...

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How To Make A Nucleic Acid

How To Make A Nucleic Acid

... amino acid of the sequence (Lys) in the manual mode. The remaining amino acid building blocks and the linker were assembled in the fully automatic ...

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Report in Nucleic Acid[1]

Report in Nucleic Acid[1]

... Orcinol Test Also called Bial's test, is a chemical test for the presence of those sugars or their derivatives which can form furfural upon heating in acidic medium. Furfural formed from pentoses reacts further with ...

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